Japanese B Encephalitis Virus in Tissue Culture.

Abstract

556JBE VIRUS IN TISSUE CULTURE ministered in stimulating or depressing dosage. I t was not possible to test the therapeutic efficacy of reserpine after serotonin administration, as the neurological symptoms induced by intra-arterially injected serotonin were of too short duration.SuInzIncIr?1. 1) Serotonin. administered into brain-tissue via an estravascular route. induces edema. possibly due to its musculotropic property. 2 ) Serotonin was confirmed to have a neurotropic action which appeared to be responsible for the reversible hemiplegic symptoms.3 ) The neurotropic effect of serotonin is biphasic. i.c. when administered in low dosage via internal-carotid artery, it causes transient spastic paralyses. In high dosage. it causes flaccid paralyses without inducing cerebral edema. 4) Chlorpromazine and reserpine both block the edema-inducing action of serotonin. Reserpine is, however, effective in a much lower dosage. 5 ) Chlorpromazine potentiates the neurotropic depressant effect of serotonin. but apparently does not alter its stirnulatins action. 6 ) Reserpine inhibits the neurotropic effect of serotonin. This holds true for the spastic as well as for the flaccid symptoms. 7) Therapeutic considerations suggest that reserpine may be specifically indicated in prophylactic treatment of apoplexy.1. Ronnameaux, Y., Lecomte, J.. A d z . Internal.

Variants of Japanese Encephalitis Virus Cytopathic for L Mouse Fibroblasts and Lass Human Epithelial Cells

“…In cultures of contiguous L mouse cells, JE viruses isolated in mice from various natural sources did not cause cytopathic effects although cytonecrosis was seen in cultures of sparse, noncontiguous cells (Dubbs and Scherer, 1966). In most human epithelial cells from continuous lines only minimal or inconsistent CPE has been described for strains Nakayama and Taira in HeLa cells (Scherer and Syverton, 1954;Mason, 1956) and for strains M1/311 and M3/2204B in HeLa, human intestine, human skin and Chang's conjunctival cells (McCollum and Foley, 1957;Banta, 1958). This note records the development of variants of JE virus with enhanced cytopathic effects (CPE) for contiguous L mouse and Lass human epithelial cells.…”