Jania adhaerens Primes Tomato Seed against Soil-Borne Pathogens

Abstract

Managing soil-borne pathogens is complex due to the restriction of the most effective synthetic fungicides for soil treatment. In this study, we showed that seed priming with Jania adhaerens water-soluble polysaccharides (JA WSPs) was successful in protecting tomato plants from the soil-borne pathogens Rhizoctonia solani, Pythium ultimum, and Fusarium oxysporum under greenhouse conditions. WSPs were extracted from dry thallus by autoclave-assisted method, and the main functional groups were characterized by using FT-IR spectroscopy. WSPs were applied by seed treatment at 0.3, 0.6 and 1.2 mg/mL doses, and each pathogen was inoculated singly in a growing substrate before seeding/transplant. Overall, WSPs increased seedling emergence, reduced disease severity and increased plant development depending on the dose. Transcriptional expression of genes related to phenylpropanoid, chlorogenic acid, SAR and ISR pathways, and chitinase and β-1,3 glucanase activities were investigated. Among the studied genes, HQT, HCT, and PR1 were significantly upregulated depending on the dose, while all doses increased PAL and PR2 expression as well as β-1,3 glucanase activity. These results demonstrated that, besides their plant growth promotion activity, JA WSPs may play a protective role in triggering plant defense responses potentially correlated to disease control against soil-borne pathogens.

Enhanced Growth and Nutritional Properties of Radish Sprouts Using Extracts from Anabaena minutissima and Sargassum vulgare

“…Some authors showed that alga extracts can enhance the growth, yield, and nutritional quality of radish plants and improve the yield, leaf photosynthesis, and ripening time of tomato. , Seaweed extracts are a mixture of biologically active substances, such as alginates, vitamins, proteins, free amino acids, polyphenols, and micro- and macro-elements that may be involved in plant growth stimulation. − Several studies have described the use of brown alga extracts to enhance the growth, yield, and nutritional quality of horticultural plants. − Some of them regard extracts from algae belonging to the Sargassum genus. , Mahmoud et al reported that radish seed and leaf treatment with Sargassum vulgare extract increased vegetative growth as well as chlorophyll and carotenoid content. On tomato, soil treatment with an extract from Sargassum horneri improved yield, leaf photosynthesis, and ripening time of tomato .…”

Seed Priming With Anabaena Minutissima Extract Ameliorates the Stress Response of Tomato Plantlets to Soilborne Pathogens

“…The disease severity caused by RS was evaluated by scoring the root apparatus based on a 0-5 scale(Righini et al 2022b), where: 0 = absence of necrosis (0% of symptoms); 1 = very slight necrosis (up to 5% of root with symptoms); 2 = slight necrosis (6-20% of root with symptoms); 3 = moderate necrosis (20-50% of root with symptoms); 4 = severe necrosis (51-70% of root with symptoms); 5 = severe crown and root necrosis (> 70% of root with symptoms The experiment was repeated three times (n = 3).…”