James McIntosh. 1882–1948

Fildes, Paul

doi:10.1002/path.1700610220

Cited by 10 publications

(9 citation statements)

References 3 publications

(3 reference statements)

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“…It was also shown that certain analogues, such as 3‐α‐indoleacrylic acid, indeed inhibits the endogenous supply (i.e. biosynthesis) of tryptophan 55. In the context of the fermentation experiments such antimetabolic actions were observed as an emergence of “linear” instead of exponential growth in the cultures supplemented with these analogues 56.…”

Section: Traditional Auxotroph‐based Methodsmentioning

confidence: 99%

Prolegomena to Future Experimental Efforts on Genetic Code Engineering by Expanding Its Amino Acid Repertoire

2004

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Protein synthesis and its relation to the genetic code was for a long time a central issue in biology. Rapid experimental progress throughout the past decade, crowned with the recently elucidated ribosomal structures, provided an almost complete description of this process. In addition important experiments provided solid evidence that the natural protein translation machinery can be reprogrammed to encode genetically a vast number of non-coded (i.e. noncanonical) amino acids. Indeed, in the set of 20 canonical amino acids as prescribed by the universal genetic code, many desirable functionalities, such as halogeno, keto, cyano, azido, nitroso, nitro, and silyl groups, as well as C=C or C[triple bond]C bonds, are absent. The ability to encode genetically such chemical diversity will enable us to reprogram living cells, such as bacteria, to express tailor-made proteins exhibiting functional diversity. Accordingly, genetic code engineering has developed into an exciting emerging research field at the interface of biology, chemistry, and physics.

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Section: Traditional Auxotroph‐based Methodsmentioning

confidence: 99%

Prolegomena to Future Experimental Efforts on Genetic Code Engineering by Expanding Its Amino Acid Repertoire

2004

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“…For example, in anoxie muscle, continued breakdown of carbohydrate results in a fall of E h and pH [6]. Necrotic muscle is highly reducing, [6,16,17] and the E h in infected muscle can fall below -41K) mV [16]. However, even minor procedures such as the injection of i(I ,o.g of adrenaline can induce a fall in Eh of 1(11 mV, [16] while 20 pg produced a fall in tissue E h to approximately -100 mV, pH 7.…”

Section: Discussionmentioning

confidence: 99%

The role of Eh, pH and iron in the bactericidal power of human plasma

Bullen

Spalding

Ward

et al. 1992

FEMS Microbiology Letters

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The bactericidal power of fresh human plasma against Klebsiella pneumoniae and Escherichia coli was extremely sensitive to changes in Eh and pH. At a high Eh (approx. +200 mV) the bacteria were destroyed, but rapid regrowth occurred when the Eh was lowered to approx. -400 mV. Abolition of the bactericidal effect was also produced by adding ferric iron at a high Eh (approx. +200 mV). Lowering the pH to 6.50 reduced or prevented the bactericidal effect. These results are probably related to the availability of iron for bacterial growth, and could be important for understanding the development of infection in injured or diseased tissue.

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“…Es zeigte sich auch, dass bestimmte Analoga, etwa 3-a-Indolylacrylsäure, die Tryptophan-Biosynthese inhibieren. [55] Bei Fermentationsexperimenten offenbarten sich antimetabolische Effekte dadurch, dass Kulturen, denen diese Analoga zugesetzt wurden, ein lineares anstelle eines exponentiellen Wachstums zeigten. [56] Dieses lineare Wachstum wurde dem Einbau der Aminosäure-Analoga in lebensnotwendige zelluläre Enzyme zugeschrieben, deren Aktivität auf diese Weise herabgesetzt oder ganz aufgehoben wurde.…”

Section: Frühe Experimenteunclassified

Prolegomena zum experimentellen Engineering des genetischen Codes durch Erweiterung seines Aminosäurerepertoires

Budiša

2004

Angewandte Chemie

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Die Proteinsynthese und ihre Verbindung mit dem genetischen Code spielten lange Zeit eine zentrale Rolle in der Biologie. Der schnelle experimentelle Fortschritt führte im vergangenen Jahrzehnt zu einer nahezu kompletten Beschreibung dieser Prozesse. Weitere wichtige Experimente ergaben Hinweise dafür, dass die natürliche Protein‐Translationsmaschinerie neu programmiert werden kann, um viele nichtkanonische Aminosäuren genetisch zu codieren. Tatsächlich lassen die 20 kanonischen Aminosäuren, die im universellen genetischen Code verwendet werden, viele wünschenswerte Funktionalitäten vermissen, etwa Halogen‐, Keto‐, Cyan‐, Azido‐, Nitroso‐, Nitro‐ oder Silylgruppen sowie CC‐ oder CC‐Bindungen. Die Fähigkeit, genetisch eine derartige chemische Vielfalt zu codieren, wird uns ermöglichen, lebende Zellen wie Bakterien neu zu programmieren, sodass sie maßgeschneiderte Proteine mit vielseitigen Funktionen exprimieren. An der Schnittstelle von Biologie, Chemie und Physik entwickelt sich mit dem Engineering des genetischen Codes ein aufstrebendes Forschungsgebiet.

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James McIntosh. 1882–1948

Cited by 10 publications

References 3 publications

Prolegomena to Future Experimental Efforts on Genetic Code Engineering by Expanding Its Amino Acid Repertoire

Prolegomena to Future Experimental Efforts on Genetic Code Engineering by Expanding Its Amino Acid Repertoire

The role of Eh, pH and iron in the bactericidal power of human plasma

Prolegomena zum experimentellen Engineering des genetischen Codes durch Erweiterung seines Aminosäurerepertoires

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