James King Hall, A. B., M. D., Ll. D

Isolation and characterization of an iron‐containing superoxide dismutase from tomato leaves, Lycopersicon esculentum

“…Activity was calculated according to Asada et al [20]. Column fractions in the course of enzyme purification were assayed as described by Henry et al [21]. Protein content of crude fractions and purified enzyme was determined according to Lowry et al [22] and Murphy and Kies I231 respectively using bovine serum albumin as a standard for both methods.…”

“…Leaf extract was applied to a column following ammonium sulfate precipitation and DEAE-Sephadex A-SO batch steps. Superoxide dismutase activity is expressed as a percentage of inhibition, by a given aliquot of the eluate, of the reaction of nitroblue tetrazolium (NBT) reduction by K f O - [21] from subsequent centrifugation (12000 x g, 1 h) was dissolved and dialyzed for 40 h against 10 mM potassium phosphate, pH 7.8, containing 5 mM 2-mercaptoethanol and 0.1 mM EDTA. The dialysate was brought to 200 mM phosphate with 1 M potassium phosphate, pH 7.8, and DEAE-Sephadex A-50 (20 m1/100 ml solution), pre-equilibrated with 200 mM potassium phosphate containing 0.1 mM EDTA, was added.…”