Jak2a regulates erythroid and myeloid hematopoiesis during zebrafish embryogenesis

Zhu, Xianmin; Liu, Rui; Guan, Jun; Zeng, Wen; Yin, Jin; Zhang, Yicheng

doi:10.7150/ijms.18907

Cited by 9 publications

(6 citation statements)

References 21 publications

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“…Once EPOR binds to EPO, it recruits JAK, a tyrosine kinase that phosphorylates EPOR. In zebra sh, over-expression of JAK/STAT3 signaling pathway promotes erythropoiesis [15][16][17][18] . Here, we found that zebra sh cuedc2 can inhibits human JAK1/STAT3 signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

miRNA-7145-cuedc2 axis controls hematopoiesis through JAK1/STAT3 signaling pathway

Guo,

Xu,

Guo

et al. 2024

Preprint

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Hematopoiesis ensures oxygen diffusion, tissue remodeling and immune protection in vertebrate. During embryogenesis, hemangioblasts are the source of all blood cells. Gata1a and pu.1 are co-existed in hemangioblasts when hemangioblasts are not differentiated into blood cells. However, the genes that determine the differentiation of hemangioblasts into myeloid or erythroid have not been fully uncovered. Here we showed that miRNA-7145, a previously unknown function miRNA, was enriched in erythrocytes at definitive wave, but not expressed in myeloid cells. Gain-of-function and loss-of-function analysis of miRNA-7145 revealed that miRNA-7145 functions as a strong inhibitor for myeloid progenitor cells differentiation while commitment for primitive erythropoiesis. Furthermore, we confirmed that cuedc2 is one of miRNA-7145 targeted-genes. Indeed, over-expression or knock-down of cuedc2 partially rescues the phenotype caused by miRNA-7145 gain-of-function or loss-of-function. Meanwhile, Gain-of-function and loss-of-function analysis of cuedc2 showed that cuedc2 is required for myelopoiesis while against to erythropoiesis. Finally, we found that over-expression of zebrafish cuedc2 in 293T cell inhibits JAK1/STAT3 signaling pathway. Collectively, our results uncover a previously unknown miRNA-7145-cuedc2 axis, which regulate hematopoiesis through inhibiting JAK1/STAT3 signaling pathway.

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Section: Discussionmentioning

confidence: 99%

miRNA-7145-cuedc2 axis controls hematopoiesis through JAK1/STAT3 signaling pathway

Guo,

Xu,

Guo

et al. 2024

Preprint

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“…The levels of STAT5 expression modulate the HSC properties, renewal versus differentiation. Recent studies using zebrafish embryos established that JAK2a might have an important role at the level of early hematopoietic progenitor/stem cells before they differentiate into distinct erythroid and myeloid lineages (Zhu et al, 2017). Similar to JAK2 V617F mutants, zebrafish JAK2AV581F mutants also show polycythemia vera phenotype (Ma et al, 2009).…”

Section: Jak/stat Signaling Pathwaymentioning

confidence: 99%

Comparative hematopoiesis and signal transduction in model organisms

Gautam

Chimata

Gutti

et al. 2021

Journal Cellular Physiology

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Hematopoiesis is a continuous phenomenon involving the formation of hematopoietic stem cells (HSCs) giving rise to diverse functional blood cells. This developmental process of hematopoiesis is evolutionarily conserved, yet comparably different in various model organisms. Vertebrate HSCs give rise to all types of mature cells of both the myeloid and the lymphoid lineages sequentially colonizing in different anatomical tissues. Signal transduction in HSCs facilitates their potency and specifies branching of lineages. Understanding the hematopoietic signaling pathways is crucial to gain insights into their deregulation in several blood‐related disorders. The focus of the review is on hematopoiesis corresponding to different model organisms and pivotal role of indispensable hematopoietic pathways. We summarize and discuss the fundamentals of blood formation in both invertebrate and vertebrates, examining the requirement of key signaling nexus in hematopoiesis. Knowledge obtained from such comparative studies associated with developmental dynamics of hematopoiesis is beneficial to explore the therapeutic options for hematopoietic diseases.

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“…Zebrafish has three homologs of JAK: jak1, jak2a, and jak2b [ 76 ]. Overexpression of jak2a in zebrafish embryos increased the expression of gata1 and hemoglobin, indicating a crucial role of jak2a in zebrafish erythropoiesis [ 77 ]. STAT proteins are transcription factors regulated by protein kinases and phosphatases.…”

Section: Using Zebrafish To Study the Regulation Of Erythropoiesismentioning

confidence: 99%

Using the Zebrafish as a Genetic Model to Study Erythropoiesis

Zhang

Chen

2021

IJMS

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Vertebrates generate mature red blood cells (RBCs) via a highly regulated, multistep process called erythropoiesis. Erythropoiesis involves synthesis of heme and hemoglobin, clearance of the nuclei and other organelles, and remodeling of the plasma membrane, and these processes are exquisitely coordinated by specific regulatory factors including transcriptional factors and signaling molecules. Defects in erythropoiesis can lead to blood disorders such as congenital dyserythropoietic anemias, Diamond–Blackfan anemias, sideroblastic anemias, myelodysplastic syndrome, and porphyria. The molecular mechanisms of erythropoiesis are highly conserved between fish and mammals, and the zebrafish (Danio rerio) has provided a powerful genetic model for studying erythropoiesis. Studies in zebrafish have yielded important insights into RBC development and established a number of models for human blood diseases. Here, we focus on latest discoveries of the molecular processes and mechanisms regulating zebrafish erythropoiesis and summarize newly established zebrafish models of human anemias.

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Jak2a regulates erythroid and myeloid hematopoiesis during zebrafish embryogenesis

Cited by 9 publications

References 21 publications

miRNA-7145-cuedc2 axis controls hematopoiesis through JAK1/STAT3 signaling pathway

miRNA-7145-cuedc2 axis controls hematopoiesis through JAK1/STAT3 signaling pathway

Comparative hematopoiesis and signal transduction in model organisms

Using the Zebrafish as a Genetic Model to Study Erythropoiesis

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