JAK2/STAT5 signaling pathway mediates Bojungbangdocktang enhanced hematopoiesis

Lim, Jeonghan; Jeong, Soo‐Jin; Koh, Wonil; Han, Ihn; Lee, Hyo‐Jung; Kwon, Tae‐Rin; Jung, Ji Hoon; Kim, Jung Hyo; Lee, Hyo‐Jeong; Lee, Eun-Ok; Kim, Sun‐Hyung; Kim, Sung‐Hoon

doi:10.1002/ptr.3257

Cited by 7 publications

(5 citation statements)

References 41 publications

(40 reference statements)

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“…Jak2 is known to have an effect on myeloid progenitors [40,41]. Moreover, the Jak2 Stat5 pathway has been implicated in the development of progenitor cells by BJBDT [36] and TSPG [37]. Further investigation is required to validate this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

“…A number of herbal samples have been shown to stimulate bone marrow progenitor cells, including polysaccharides from Angelica sinensis [35], a medicinal herbal cocktail Bojungbangdocktong (BJBDT) [36], a polysaccharide fraction from black soybean [36], and total saponins of Panax ginseng cells (TSPGs) [37]. We are the first to describe the stimulation of myelopoiesis activity by aerial parts of Echinacea species in female Sprague-Dawley rats [26].…”

Section: Discussionmentioning

confidence: 99%

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1H-NMR-Based Chemometric Analysis of Echinacea Species to Predict Effectors of Myeloid Progenitor Stimulation

Nagumalli,

Salley,

Carstens

2024

Analytica

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Echinacea, a herbaceous, perennial flowering plant from the Compositae (Asteraceae) family, exhibits stimulating effects on myeloid progenitors (CFU-GMs) in rat bone marrow, as demonstrated in our previous study using a 75% (v v−1) ethanol extract of aerial parts. Expanding on this work, we have investigated eleven different Echinacea samples that belong to three species for their myeloid progenitor-stimulating activity. Simultaneously, we employed 1H-NMR spectroscopy (400 MHz, 0.02–10.02 ppm) and chemometric analysis to predict constituents responsible for activity. Female Sprague–Dawley rats received oral doses of ethanol extracts (0–200 mg extract dry weight kg−1 body weight) of Echinacea for seven days. Bone marrow cells were then cultured with CFU-GM growth factors. Extracts showing a statistically significant (p < 0.05) increase in CFU-GM, compared to the control, were considered active. Significant CFU-GM increases were observed in rats treated with seven Echinacea samples, ranging from 39% to 91% higher than the control, while four samples were inactive. All five Echinacea purpurea samples showed myeloid progenitor-stimulating activity, while one sample each of Echinacea angustifolia and Echinacea pallida also exhibited the same activity. By applying orthogonal partial least squares discriminant analysis (OPLS-DA) to the 1H-NMR spectra, we identified specific spectral bins (0.70–1.98 ppm aliphatic and 6.38–7.76 ppm aromatic) correlating with myelopoiesis stimulation. These findings highlight the potential of chemometric analysis using 1H-NMR spectroscopy to infer the chemical classes that could be responsible for the bioactive properties of complex herbal mixtures, like Echinacea.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

1H-NMR-Based Chemometric Analysis of Echinacea Species to Predict Effectors of Myeloid Progenitor Stimulation

Nagumalli,

Salley,

Carstens

2024

Analytica

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“…In recent years, the spectrum-effect relationship has been successfully applied to evaluate the bioactive material basis, with common data processing methods such as principal component analysis (PCA), canonical correlation analysis (CCA), gray correlation analysis (GRA), bivariate correlation analysis (BCA), and orthogonal partial least-squares regression analysis (OPLSR) [ 18 , 40 ]. Among them, the OPLSR method has high applicability when the datasets were small [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…The BD mouse model generated by 1-acetyl-2-phenylhydrazine (APH) coupled with cyclophosphamide (CTX) is commonly adopted to mimic the symptom of BD, such as weight loss, and decrease of blood routine indicators [ 12 – 15 ]. Therefore, blood routine indicators like HCT, HGB, and RBC were widely utilized to assess the blood tonic efficacy of drugs [ 16 – 18 ]. Besides, plant metabolomics enables the comprehensive comparison of the chemical constituents by combining the state-of-the-art high-resolution MS-based techniques with multivariate data analysis [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of blood tonic efficacy and chemical constituents of Kadsura interior A.C. Smith and its closely related species

Liu

et al. 2022

Chin Med

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Background The stems of Kadsura interior A. C. Smith are used as traditional Chinese medicine (TCM) Kadsurae Caulis, with the traditional efficacy of tonifying and invigorating the blood, therefore being favored to treat blood deficiency (BD) widely. However, the stems of K. interior and its closely related species are morphologically similar and they may readily be misused as Kadsurae Caulis, thus likely to exert negative effects on clinical efficacy and clinical medication safety. Methods Firstly, blood tonic efficacies of the stems of K. interior (KIS) and its closely related species were compared using BD mouse model induced by 1-acetyl-2-phenylhydrazine (APH) and cyclophosphamide (CTX). Secondly, the chemical constituents from the stems of K. interior and its closely related species were evaluated and compared using a plant metabolomics approach. Plant metabolomics in this study aims at discovering differential metabolites and comprehensively assessing the chemical constituents by combining state-of-the-art high-resolution UPLC-Q/TOF–MS/MS technique and multivariate data analysis. Finally, based on the pharmacological data and the chemical constituents in UPLC-Q/TOF–MS fingerprints, the potential blood tonic active markers were screened by the spectrum-effect relationship analysis and quantified by UPLC-UV-DAD. Results The ethanol extract of the stems of K. interior significantly increased the levels of hematocrit (HCT), hemoglobin (HGB), and red blood cells (RBC) in BD mice. In addition, it significantly increased the serum levels of interleukin 3 (IL-3), granulocyte–macrophage colony-stimulating factor (GM-CSF), and macrophage-stimulating factor (M-CSF) in BD mice (P < 0.01). The blood tonic efficacy of the stems of K. interior was superior to those of its closely related species, especially at the dose of 200 mg/kg. Six differential compounds in the stems of K. interior were screened out to distinguish it from its closely related species. In combination with the results of the spectrum-effect relationship analysis, heteroclitin D, interiorin C, and heteroclitin G were identified as potential bioactive markers. The contents of heteroclitin D and heteroclitin G in the freeze-dried powder of KIS were 15.90 and 3.74 μg/mg. Conclusions This study illustrated the differences in the blood tonic efficacies and the chemical constituents of the stems of K. interior and its closely related species, and pinpointed the potential bioactive markers of K. interior.

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“…Bo-jung-bang-dock-tang (BJBDT) is a medicinal herbal cocktail that has been used for cancer prevention and treatment in traditional Korean medicine. Lim et al [44] suggested that BJBDT can enhance hematopoiesis via the hematopoietic cytokine-mediated JAK2/STAT5 pathway. BJBDT significantly increased expression of the hematopoietic cytokines interleukin (IL-3), stem cell factor (SCF), granulocyte-macrophage-colony stimulating factor (GM-CSF), thrombopoietin (TPO), and erythropoietin (EPO) at the level of mRNA and secretion in hematopoietic stem cells (HSCs).…”

Section: Hematopoietic Stem Cells In High-dose Chemotherapymentioning

confidence: 99%

Stem Cells, Cancer Stem-Like Cells, and Natural Products

Efferth

2012

Planta Med

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Somatic stem cells can be found in many rapidly regenerating tissues, e.g., the skin, gastrointestinal mucosa, and hematopoietic system, but are also present at low numbers in non-regenerative organs such as the heart and brain. They function in maintaining normal tissue homeostasis and in tissue repair after injury as well as self-renewal and the generation of specific progenitor cells during differentiation. If needed, somatic stem cells can switch from a quiescent state to a highly proliferative state, differentiate to functionally mature cells and replace other differentiated cells during physiological tissue turnover [1-4]. One of the most promising applications of stem cell biology is tissue regeneration. The concept is to differentiate stem cells into specific tissue and cell types in order to treat neurodegenerative diseases, muscular diseases, diabetes, and others. The challenge in regenerative medicine is the targeted differentiation of stem cells into the desired cell type. Embryonic stem cell-like induced pluripotent stem cells (iPSC) can be generated by inducing expression of defined transcription fac

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JAK2/STAT5 signaling pathway mediates Bojungbangdocktang enhanced hematopoiesis

Cited by 7 publications

References 41 publications

1H-NMR-Based Chemometric Analysis of Echinacea Species to Predict Effectors of Myeloid Progenitor Stimulation

1H-NMR-Based Chemometric Analysis of Echinacea Species to Predict Effectors of Myeloid Progenitor Stimulation

Comparison of blood tonic efficacy and chemical constituents of Kadsura interior A.C. Smith and its closely related species

Stem Cells, Cancer Stem-Like Cells, and Natural Products

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