JAK and STAT members of yellow catfish Pelteobagrus fulvidraco and their roles in leptin affecting lipid metabolism

Wu, Kun-Lung; Tan, Xiao-Ying; Xu, Yi-Huan; Chen, Qiliang; Pan, Ya–Xiong

doi:10.1016/j.ygcen.2015.12.014

Cited by 27 publications

(37 citation statements)

References 52 publications

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“…However, limited information is available on STATs in fish. To our knowledge, full-length cDNA sequence of STAT repertoires have been determined in mandarin fish, Siniperca chuatsi [ 2 ], and yellow catfish, Pelteobagrus fulvidraco [ 3 ], although certain members have been obtained in other fishes [ 4 , 5 , 6 , 7 , 8 ]. In addition, due to the whole genome duplication (WGD) event of the modern bony fish [ 9 ], additional STAT members may have arisen in teleost.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Expression Analysis of STAT Members from Synechogobius hasta and Their Roles in Leptin Affecting Lipid Metabolism

Tan

Wei

et al. 2016

IJMS

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Signal transducers and activators of transcription proteins (STATs) act as important mediators in multiple biological processes induced by a large number of cytokines. In the present study, full-length cDNA sequences of seven STAT members, including some splicing variants different from those in mammals, were obtained from Synechogobius hasta. The phylogenetic analysis revealed that the seven STAT members were derived from paralogous genes that might have arisen by whole genome duplication (WGD) events during vertebrate evolution. All of these members share similar domain structure compared with those of mammals, and were widely expressed across the tested tissues (brain, gill, heart, intestine, liver, muscle and spleen), but at variable levels. Incubation in vitro of recombinant human leptin changed the intracellular triglyceride (TG) content and mRNA levels of several STATs members, as well as expressions and activities of genes involved in lipid metabolism. Furthermore, Tyrphostin B42 (AG490), a specific inhibitor of the Janus Kinase 2(JAK2)-STAT pathway, partially reversed leptin-induced change on STAT3 and its two spliced isoforms expression, as well as expressions and activities of genes involved in lipid metabolism. As a consequence, the decrease of TG content was also reversed. Thus, our study suggests that STAT3 is the requisite for the leptin signal and the activation of the STAT3 member may account for the leptin-induced changes in lipid metabolism in S. hasta.

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Section: Introductionmentioning

confidence: 99%

Isolation and Expression Analysis of STAT Members from Synechogobius hasta and Their Roles in Leptin Affecting Lipid Metabolism

Tan

Wei

et al. 2016

IJMS

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“…Additionally, leptin reduces Aβ levels in vitro and in vivo [5][6][7]. Sets of evidence show that leptin reduces Aβ production and tau phosphorylation in neuronal cells and transgenic mice models of AD [8,9]. Fewlass et al has found the leptin's ability to modify Aβ levels in vitro and in vivo [10].…”

Section: Introductionmentioning

confidence: 99%

Leptin Regulates Tau Phosphorylation through Wnt Signaling Pathway in PC12 Cells

Zhang

Guo²,

Zhang³

et al. 2016

Neurosignals

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Background/Aims: Leptin, an adipocytokine produced endogenously in the brain, is decreased in Alzheimer's disease(AD) and has also been shown to reduce Aβ levels in vitro and in vivo. Sets of evidence show that leptin reduces Aβ production and tau phosphorylation in neuronal cells and transgenic mice models of AD. Herein, we investigated the signaling pathway activated by leptin, to better understand its mechanism of action. Methods: Western blotting was performed to assess the levels of phosphor-tau and Bax, RT-PCR to check the mRNA level of Bax. Results: Leptin treatment significantly blunted Aβ-evoked tau phosphorylation and Bax levels, effects of which could be reversed by antagonist of Wnt signaling. Conclusion: The data indicate that Leptin may provide a novel therapeutic approach to AD treatment via wnt signaling.

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“…Phosphorylation of tyrosine residue Tyr1138 recruits transcription factor STAS3 to become a substrate for receptor-related JAKs. Phosphorylated STATs separate from the receptors and form activated dimers, which are then translocated to the promoter of the target gene in the nucleus to regulate gene expression and participate in energy balance and metabolic regulation [18][19][20][21]. A previous study has shown that either mutation of Tyr985 or Tyr1077 residues of leptin receptors leads to the weakening of STAT3 phosphorylation activation and affects signal transduction, while the simultaneous mutation of these two residues blocks signal transduction and induces leptin resistance [22].…”

Section: Discussionmentioning

confidence: 99%

OB-RGRP regulates the phosphorylation of JAK2 and STAT3 in primary rat adipocytes

Shi

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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This study aimed to explore the role of obestatin R gene-related protein (OB-RGRP) in autocrine signal transduction of adipocytes. Primary rat adipocytes were isolated and verified by microscopic observation and oil red O staining. OB-RGRP expression vector and OB-RGRP siRNA (si-OB-RGRP) were constructed and transfected into adipocytes. Adipocytes were then divided into five groups: (1) Control; (2) Vector (empty expression vector); (3) OB-RGRP (OB-RGRP expression vector); (4) si-OB-RGRP NC (si-OB-RGRP negative control); (5) si-OB-RGRP. mRNA and protein levels of OB-RGRP, JAK2, phosphorylated JAK2 (p-JAK2), STAT3 and phosphorylated STAT3 (p-STAT3) were examined using RT-PCR and western blot, respectively. Results showed that mRNA and protein levels of OB-RGRP in the Vector and si-OB-RGRP NC groups were similar to those in the Control group. Their levels in the si-OB-RGRP and OB-RGRP groups were significantly down-regulated and up-regulated (p < .05), respectively, in comparison with the control cells. There was no significant difference in the mRNA and protein levels of JAK2 and STAT3 among various groups. Moreover, the si-OB-RGRP NC and Vector groups induced similar ratios of p-JAK2 to JAK2 (p-JAK2/JAK2) and p-STAT3 to STAT3 (p-STAT3/STAT3) to the Control group. However, these two ratios in the si-OB-RGRP and OB-RGRP groups were significantly reduced and elevated (p < .05), respectively, in comparison with the Control group. These results suggested that OB-RGRP overexpression enhanced the levels of p-JAK2 and p-STAT3 while OB-RGRP silencing lowered their levels. In conclusion, OB-RGRP regulated the phosphorylation of JAK2 and STAT3 in primary rat adipocytes. ARTICLE HISTORY

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JAK and STAT members of yellow catfish Pelteobagrus fulvidraco and their roles in leptin affecting lipid metabolism

Cited by 27 publications

References 52 publications

Isolation and Expression Analysis of STAT Members from Synechogobius hasta and Their Roles in Leptin Affecting Lipid Metabolism

Isolation and Expression Analysis of STAT Members from Synechogobius hasta and Their Roles in Leptin Affecting Lipid Metabolism

Leptin Regulates Tau Phosphorylation through Wnt Signaling Pathway in PC12 Cells

OB-RGRP regulates the phosphorylation of JAK2 and STAT3 in primary rat adipocytes

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