Jacalin-copper sulfide nanoparticles complex enhance the antibacterial activity against drug resistant bacteria via cell surface glycan recognition

Ahmed, Khawaja Ashfaque; Subramaniyan, Siva Bala; Banu, Sanaulla Farisa; Nithyanand, Paramasivam; Veerappan, Anbazhagan

doi:10.1016/j.colsurfb.2017.12.053

Cited by 38 publications

(48 citation statements)

References 38 publications

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“…However, blocking the sugar binding site of jacalin with galactose reverses the MIC from 0.78 to 25 μM, indicating the importance of a galactose-binding site of jacalin for bacterial recognition. 23…”

Section: Resultsmentioning

confidence: 99%

“…22 The glycan recognition properties of jacalin were reported for enhancing the antibacterial activity of N -lauryl tyramine-capped CuS NPs (NLTA-CuS NPs) against drug resistance bacteria. 23 Herein, we demonstrated through a battery of biophysical and biochemical methods that the interference from the protein corona on the antibacterial activity of pCuS could be diminished by functionalizing the NPs with jacalin. To the best of our knowledge, this is the first study reporting a novel method based on lectins to curtail the protein corona interference in the antibacterial activity of NPs.…”

Section: Introductionmentioning

confidence: 99%

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Remarkable Effect of Jacalin in Diminishing the Protein Corona Interference in the Antibacterial Activity of Pectin-Capped Copper Sulfide Nanoparticles

et al. 2019

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Herein, we report a new strategy based on jacalin functionalization to diminish the impact of biological fluids in the antibacterial applications of nanoparticles (NPs). Precoating pectin-capped copper sulfide NPs (pCuS) with bovine serum albumin produced a protein corona, which affects the antibacterial activity of pCuS. It was found that the minimum inhibitory concentration (MIC) increases fourfold because of the formation of the protein corona. Interestingly, the pCuS functionalized with jacalin enhance the targeting capabilities through bacterial cell surface glycan recognition with no interference from the protein corona. The MIC of pCuS decreases 16-fold on functionalization with jacalin. Mechanistic studies indicated that the pCuS functionalized with jacalin impede the protein corona interference and induce bacterial cell death by impairing the GSH/reactive oxygen species balance and disrupting the bacteria cell membrane. As a proof of concept, we used a bacteria-infected zebrafish animal model to demonstrate the interference of biological fluids in the antibacterial activity of NPs. Infected zebrafish treated with 1× MIC of pCuS failed to recover from the infection, but 4× MIC rescues the fish. The requirement of a high dose of NPs to treat the infection confirms the interference of biological fluids in nanotherapeutic applications. At the same time, the jacalin–pCuS complex rescues the infected fish at 16-fold lesser MIC. The results obtained from this study suggest that jacalin-mediated NP targeting may have broad implications in the development of future nanomedicine.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Remarkable Effect of Jacalin in Diminishing the Protein Corona Interference in the Antibacterial Activity of Pectin-Capped Copper Sulfide Nanoparticles

et al. 2019

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“…These interactions are presented as ligplot in figure 4 and suggest that jacalin has the binding pocket for hydrophobic drugs, which can be used for the development of jacalin-based drug carrier to target the RBD. To support the global research effort of finding solutions for COVID-19 drives us to propose jacalin, because the recent study showed that jacalin-CuS NPs (JCuS NPs) eliminate the bacterial infection in zebra fish model [12]. The bacterial cell surface glycan recognition activity of jacalin plays an important role in targeting the antibacterial NPs.…”

Section: Resultsmentioning

confidence: 99%

“…To support the global research effort of finding solutions for COVID-19 drives us to propose jacalin, because the recent study showed that jacalin-CuS NPs (JCuS NPs) eliminate the bacterial infection in zebra fish model [ 12 ]. The bacterial cell surface glycan recognition activity of jacalin plays an important role in targeting the antibacterial NPs.…”

Section: Resultsmentioning

confidence: 99%

“…Jacalin, a 66 kDa homotetrameric lectin isolated from the seeds of jackfruit, was reported to block the HIV-1 infection of CD4 + lymphoblastoid cells [11]. Recently, we found that jacalin recognizes the bacterial cell surface glycan and enhances the antimicrobial activity of nanoparticles [12]. Jacalin capped platinum nanoparticles exhibit excellent antimicrobial activity and promote adaptive immune response in a host to suppress repeated bacterial infection [13].…”

Section: Introductionmentioning

confidence: 99%

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Targeting the glycan of receptor binding domain with jacalin as a novel approach to develop a treatment against COVID-19

2020

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Photosynthesis of hybrid silver‐based nanoparticles mediated lectins and evaluation of their hemagglutinating properties

Fernandes

Capo

Tofanello

et al. 2022

Biotech and App Biochem

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Lectins are carbohydrate‐binding proteins belonging to the Leguminosae family. In this family stand out proteins extracted from species belonging to Diocleinae subtribe, which includes, for example, the seed lectin from Dioclea violacea (DVL) and the jack bean lectin Concanavalin A (ConA). Here, we report the photosynthesis of silver/silver chloride nanoparticles (NPs) assisted by ConA and DVL. The syntheses were simple processes using a green‐chemistry approach. Under electron microscopy, NPs heterogeneous in size, nearly spherical and covered by a thin lectin corona, were observed. Both NPs assisted by lectins were capable to cause strong rabbit erythrocytes agglutination with the same titers of hemagglutinating activities. These results indicate that both lectins maintained their biological activities even after association with the NPs and therefore are able to interact with biological membrane carbohydrates. However, for rabbit erythrocytes treated with proteolytic enzymes were observed different titers of hemagglutinating activities, suggesting differences in the spatial arrangement of the lectins on the surface of the NPs. This study provides evidences that these hybrid lectin‐coated silver/silver chloride NPs can be used for selective recognition and interaction with membrane carbohydrates and others biotechnological applications.

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Jacalin-copper sulfide nanoparticles complex enhance the antibacterial activity against drug resistant bacteria via cell surface glycan recognition

Cited by 38 publications

References 38 publications

Remarkable Effect of Jacalin in Diminishing the Protein Corona Interference in the Antibacterial Activity of Pectin-Capped Copper Sulfide Nanoparticles

Remarkable Effect of Jacalin in Diminishing the Protein Corona Interference in the Antibacterial Activity of Pectin-Capped Copper Sulfide Nanoparticles

Targeting the glycan of receptor binding domain with jacalin as a novel approach to develop a treatment against COVID-19

Photosynthesis of hybrid silver‐based nanoparticles mediated lectins and evaluation of their hemagglutinating properties

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