Jaboticaba peel extract as an antimicrobial agent: screening and stability analysis

Abstract

PurposeThis study aimed to evaluate the antimicrobial activity of the aqueous extract from jaboticaba skin against important foodborne bacteria and fungi and its stability.Design/methodology/approachJaboticaba skin aqueous extract (at ratio of 10 g L-1) was tested against Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus and Escherichia coli. Stability of the anti-staphylococcal activity, total phenolics, monomeric anthocyanins, tannins, phenolic acid content were measured and statistically correlated.FindingsThe residue extract inhibited L.monocytogenes, S.aureus, B.cereus and E.coli growth but was not effective against fungi and was stable to the thermal treatments, remaining with its inhibitory activity against S.aureus. When stored for 14 days at 25 °C with an incidence of light, there was a reduction in the antibacterial activity and in the phenolic compounds. The change in pH slightly changed polyphenolic content profile, and the exposure to papain and bromelain did not affect the antimicrobial activity. Results showed strong correlation between anti-staphylococcal activity, the presence of polyphenols and anthocyanins, meanwhile moderate correlation with phenolic acids content in the extract.Originality/valueBiopreservatives are a great trend in food microbiology. The present work shows deeper information about the utilization of jaboticaba skin as antimicrobial agent and its stability, which is not found in the current literature.

Antimicrobial effect of phenolic-rich jaboticaba peel aqueous extract on Staphylococcus aureus and Escherichia coli

“…Jaboticaba peel extracts were obtained as described previously (Caxambú et al, 2016;Trojaike et al, 2019;Fleck et al, 2021). Samples of 3 g of dried jaboticaba peels were kept in contact with 30 mL boiling distilled water for 10 min in sealed flask with constant agitation, when the samples were filtered on Whatman filter paper nº1 for falcon tubes with a lid under aseptic conditions.…”

“…Other health benefits include increasing HDL cholesterol, i.e., having a positive effect on the cardiovascular system and antidiabetic properties (Wu et al, 2012;Lenquiste et al, 2012Lenquiste et al, , 2015. Besides the well-documented human health-promoting effects of bioactive compounds obtained from jaboticabas, the antimicrobial activity of some polyphenols has been reported, representing an interesting tool to be used as natural preservatives in the food processing chain (Lacombe et al, 2010;Caxambú et al, 2016;Trojaike et al, 2019;Fleck et al, 2021).…”

“…Compounds from jaboticaba peels have shown interesting antimicrobial activity against important foodborne bacteria such as Escherichia coli, Salmonella enterica, Bacillus cereus, Staphylococcus aureus and Listeria monocytogenes (Hacke et al, 2016;Oliveira et al, 2018;Fleck et al, 2021). Fleck et al (2021) recently observed that anti-staphylococcal activity of compounds extracted from jaboticaba peels are stable to pH changes, thermal treatments, and light exposure and this capacity is closely related to the concentration of monomeric anthocyanins and phenolic compounds. The major compounds in jaboticaba peels are consisted of tannins and anthocyanins, mainly cyanidin 3-glucoside, delphinidin-3-glucoside and trigalloylglucose (Quatrin et al, 2019).…”

“…Aiming at practical applications, utilization of aqueous extracts to obtain target compounds from food matrix is highly important, since water is a safe, abundant, cheap solvent and does not involve environmental impact as well as discussing common knowledge regarding the food chain actors (Sant'Anna et al, 2017;Fleck et al, 2021). So far, no data is found in current literature evaluating antimicrobial mode of action of an aqueous extract of this fruit residue.…”

Brazilian Agroindustrial Wastes as a Potential Resource of Bioative Compounds and Their Antimicrobial and Antioxidant Activities

“…Thus, once the bioactive compounds are extracted, they can be used as natural additives. Although the present compounds are of great interest, their effective use requires some pre-treatments of the raw materials [ 1 , 15 , 18 , 23 , 24 , 25 ]. An example of the pre-treatment is drying, which can improve postharvest life relatively low in fresh plant matrices.…”