Jaboticaba (Myrciaria jaboticaba) powder consumption improves the metabolic profile and regulates gut microbiome composition in high-fat diet-fed mice

Soares, Elaine; Soares, Aruanna Cajaty; Trindade, Patrícia Leticia; Monteiro, Elisa Bernardes; Martins, Fabiane Ferreira; Forgie, Andrew J.; Inada, Kim Ohanna Pimenta; de, Graziele Freitas; Resende, Ângela Castro; Perrone, Daniel; Souza-Mello, Vanessa; Tomás‐Barberán, Francisco A; Willing, Benjamin P.; Monteiro, Mariana; Daleprane, Julio Beltrame

doi:10.1016/j.biopha.2021.112314

Cited by 19 publications

(18 citation statements)

References 54 publications

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“…Thirteen strains of bacteria were significantly enriched in the CDAHFD group, and the relative abundance of Clostridia , Erysipelatoclostridiaceae , Romboutsia_ilealis , Peptostreptococcales_Tissierellales , and Peptostreptococcaceae was relatively high. A previous study reported that Peptostreptococcaceae was significantly elevated in NAFLD mice induced by HFD ( Soares et al, 2021 ), which is consistent with our findings. Ten strains of bacteria that were significantly enriched in the AEPE-M group were as follows: unidentified_Bacteria , Bacteroides , Bacteroidaceae , Bacteroides_acidifaciens, Verrucomicrobiae , Verrucomicrobiota , Verrucomicrobiales , Akkermansiaceae , Akkermansia_muciniphila , and Akkermansia .…”

Section: Resultssupporting

confidence: 93%

“…A previous study reported that Peptostreptococcaceae was significantly elevated in NAFLD mice induced by HFD (Soares et al, 2021), which is consistent with our findings. Ten strains of bacteria that were significantly enriched in the AEPE-M group were as follows: unidentified_Bacteria, Bacteroides, Bacteroidaceae, Bacteroides_acidifaciens, Verrucomicrobiae, Verrucomicrobiota, Verrucomicrobiales, Akkermansiaceae, Akkermansia_muciniphila, and Akkermansia.…”

Section: Aepe-m Regulates the Composition Of The Gut Microbiota That ...supporting

confidence: 93%

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Phyllanthus emblica aqueous extract retards hepatic steatosis and fibrosis in NAFLD mice in association with the reshaping of intestinal microecology

et al. 2022

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Accumulating evidence suggests that dysregulation of the intestinal flora potentially contributes to the occurrence and development of nonalcoholic fatty liver disease (NAFLD). Phyllanthus emblica (PE), an edible and medicinal natural resource, exerts excellent effects on ameliorating NAFLD, but the potential mechanism remains unclear. In the present study, a mouse NAFLD model was established by administering a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD). The protective effects of the aqueous extract of PE (AEPE) on the gut microbiota and fecal metabolites in NAFLD mice were detected by performing 16S rRNA gene sequencing and untargeted metabolomics. The administration of middle- and high-dose AEPE decreased the levels of ALT, AST, LDL-C, TG, and Hyp and increased HDL-C levels in CDAHFD-fed mice. Hematoxylin–eosin (H&E), Oil Red O, and Masson’s trichrome staining indicated that AEPE treatment attenuated hepatic steatosis and fibrotic lesions. Moreover, the disordered intestinal microflora was remodeled by AEPE, including decreases in the abundance of Peptostreptococcaceae, Faecalibaculum, and Romboutsia. The untargeted metabolomics analysis showed that AEPE restored the disturbed glutathione metabolism, tryptophan metabolism, taurine and hypotaurine metabolism, and primary bile acid biosynthesis of the gut bacterial community in NAFLD mice, which strongly correlated with hepatic steatosis and fibrosis. Collectively, AEPE potentially ameliorates NAFLD induced by a CDAHFD through a mechanism associated with its modulatory effects on the gut microbiota and microbial metabolism.

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Section: Resultssupporting

confidence: 93%

Section: Aepe-m Regulates the Composition Of The Gut Microbiota That ...supporting

confidence: 93%

Phyllanthus emblica aqueous extract retards hepatic steatosis and fibrosis in NAFLD mice in association with the reshaping of intestinal microecology

et al. 2022

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“…An increased abundance of Akkermansia induced in response to cranberry extract has been reported to be protective against hepatic steatosis and insulin resistance . In another study, the administration of polyphenol-rich jaboticaba peel and seed powder produced beneficial effects on NAFLD associated with an increased abundance of Akkermansia genera . Moreover, Akkermansia reduced the serum TG and ALT levels in HFD-fed mice and the expression of the SREBP and IL-6 genes, resulting in decreased lipid production and inflammation in the liver, respectively .…”

Section: Discussionmentioning

confidence: 94%

The Ameliorating Effects of Apple Polyphenol Extract on High-Fat-Diet-Induced Hepatic Steatosis Are SIRT1-Dependent: Evidence from Hepatic-Specific SIRT1 Heterozygous Mutant C57BL/6 Mice

Yin

Liu

et al. 2022

J. Agric. Food Chem.

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Apple polyphenol extract (APE) has been reported to possess protective effects against hepatic steatosis. To explore whether APE-induced alleviation of hepatic steatosis is SIRT1-dependent, the present study was carried out using wild-type and hepatic SIRT1 heterozygous mutant (Sirt1+/–) C57BL/6 mice. On consideration of the sex disparity related to hepatic steatosis morbidity, both male and female mice were included in the study. Six to eight week old mice were fed a high-fat diet (HFD) and randomly assigned to one of the following groups: (1) wild-type mice (wt+HFD), (2) Sirt1+/– mice (Sirt1+/–+HFD), and (3) Sirt1+/– mice with 500 mg/(kg·bw·d) APE intragastric administration (Sirt1+/–+HAP). HFD-induced weight gain and triglyceride accumulation was more prominent in Sirt1+/– mice in comparison to wild-type mice. Following APE treatment, these effects were significantly reduced along with the alleviation of hepatic steatosis via upregulated expression of SIRT1 at the protein and mRNA levels in both male and female mice. However, APE differentially regulated the genes related to lipid metabolism (Lkb1, Ampk, Hsl, Srebp-1c, Abcg1, and Cd36) in a sex-specific manner. Moreover, APE treatment altered gut microbiota composition, with an increased relative abundance of Akkermansia and a decreased Firmicutes/Bacterodetes ratio. Thus, our study provided new evidence supporting our hypothesis that APE-induced alleviation of hepatic steatosis is SIRT1-dependent.

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“…The phytochemical-induced mucin phenotype may partly explain their increased abundance in the gut; however, the absence of A. muciniphila in the PA diet suggests other factors contributed to their increase in the DW group. A study in mice using jaboticaba fruit, which is high in flavan-3-ols, at 5%, 10%, and 15% of diet found an increase in A. muciniphila at only 10% and 15% (36) suggesting a dose-dependent threshold that supports their growth likely exists in the gut. The lack of A. muciniphila in the PA group of this present study is inconsistent with our previous study (12) and suggest mice did not receive the necessary dose of polyphenols to encourage A. muciniphila fitness in the gut.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical-induced mucin accumulation in the gastrointestinal lumen is independent of the microbiota

Willing

Forgie

et al. 2022

Preprint

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The mucus layer is critical to gastrointestinal health and ecology. Dietary phytochemicals are well documented to stimulate mucus production and secretion, but the underlying mechanism and effects on gut health are poorly understood. We fed germ-free and conventional mice diets containing approximately 0.4% of polyphenols per gram to determine if the phytochemical-induced accumulation of mucin in the gastrointestinal lumen is dependent on the microbiota. In addition, we assess how increased mucin shapes microbial communities in conventional mice. Germ-free mice receiving a pea ( Pisum sativuum ) seed coat proanthocyanidin-containing diet (PA) had greater levels of fecal mucin compared to the non-proanthocyanidin-containing (NPA) pea seed coat diet control ( P < 0.05), confirming that fecal mucin accumulation is independent of the gut microbiota. Conventional mice fed the PA diet and a red osier dogwood (ROD; Cornus sericea ) extract diet (DW) had higher mucin levels compared to a control diet without phytochemicals ( P < 0.01 and P < 0.05, respectively). The increase in luminal mucin was associated with consistent increases in bacterial taxa belonging to Lachnospiraceae and [ Clostridium ] leptum species and a decrease in Romboutsia species. We conclude that phytochemicals have the ability to alter gut microbial ecology by increasing the amount of mucin in the gastrointestinal lumen.

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Jaboticaba (Myrciaria jaboticaba) powder consumption improves the metabolic profile and regulates gut microbiome composition in high-fat diet-fed mice

Cited by 19 publications

References 54 publications

Phyllanthus emblica aqueous extract retards hepatic steatosis and fibrosis in NAFLD mice in association with the reshaping of intestinal microecology

Phyllanthus emblica aqueous extract retards hepatic steatosis and fibrosis in NAFLD mice in association with the reshaping of intestinal microecology

The Ameliorating Effects of Apple Polyphenol Extract on High-Fat-Diet-Induced Hepatic Steatosis Are SIRT1-Dependent: Evidence from Hepatic-Specific SIRT1 Heterozygous Mutant C57BL/6 Mice

Phytochemical-induced mucin accumulation in the gastrointestinal lumen is independent of the microbiota

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