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Abstract

ABSTRACT:Tecomella undulata is one of the most important agroforestry species of arid region.The effects of different concentrations of NH4NO3, KNO3, NH4NO3 X KNO3 and Glutamine were investigated on proliferation stage of T. undulata cultivated on MS media as nitrogen sources. The highest shoot length and multiplication was obtained with combination of NH4NO3 X KNO3 (10.31 mM X 9.40 mM) with low hyperhydricity (10.3%). Overall, the MS N combination was superior to any single N source for proliferation and growth of shoots. The explants cultured with other nitrogen sources resulted in low culture frequency and low number of shoots per explant accompanied by basal callusing and hyperhydricity. This study also demonstrates that organic source of N as glutamine does not show any improvement in shoot growth and hyperhydricity.