J‐Aggregate‐Based FRET Monitoring of Drug Release from Polymer Nanoparticles with High Drug Loading

Abstract

Understanding drug-release kinetics is critical for the development of drug-loaded nanoparticles.W edeveloped aJaggregate-based Fçrster-resonance energy-transfer (FRET) method to investigate the release of novel high-drug-loading (50 wt %) nanoparticles in comparison with low-drug-loading (0.5 wt %) nanoparticles.S ingle-dye-loaded nanoparticles form J-aggregates because of the high dye-loading (50 wt %), resulting in al arge red-shift (% 110 nm) in the fluorescence spectrum. Dual-dye-loaded nanoparticles with high dye-loading using FRET pairs exhibited not only FRET but also aJaggregate red-shift (116 nm). Using this J-aggregate-based FRET method, dye-core-polymer-shell nanoparticles showed two release processes intracellularly:the dissolution of the dye aggregates into dye molecules and the release of the dye molecules from the polymer shell. Also,t he high-dye-loading nanoparticles (50 wt %) exhibited as low release kinetics in serum and relatively quickrelease in cells,demonstrating their great potential in drug delivery.

J‐Aggregation‐Driven Supramolecular Assembly of Dye‐Conjugated Block Polymers: From Morphological Tailoring to Anticancer Applications

“…[1][2][3] Compared with the monomer, J-aggregate gains multiple photophysical advantages, which are beneficial for in vivo antitumor biomedical applications, such as red-shifted spectral lines, increased absorptivity, and narrowed absorption and emission bands. [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] However, without a suitable nanocarrier or scaffold, the below, we discovered that the conjugated BODIPY can form J-aggregate and drive the supramolecular assembly of the polymer matrix to yield 1D filaments only when the hydrophobic segment is short, which is not applicable in polymers with long hydrophobic blocks (Figure 1a). We proposed here a new strategy of using BODIPY small molecules as a molecular glue to recover the J-stacking of the conjugated BODIPYs, thereby directing the assembly of the polymer matrix with a long hydrophobic block (Figure 1b).…”

Implications of Quenching‐to‐Dequenching Switch in Quantitative Cell Uptake and Biodistribution of Dye‐Labeled Nanoparticles

“…[10] Some studies have utilized these quenching and de-quenching effects at high dye loading for stimuli-responsive sensing applications [11] as well as monitoring dye release from polymer NPs. [12] However,t he quenching effect at low dye loadings has rarely been taken into consideration during experimental design. More surprisingly many studies did not even mention the dye loading of NPs, [13] which could lead to wrong interpretation of data thus misleading conclusions.…”

Na6MQ4 (M=Zn, Cd; Q=S, Se): Promising New Ternary Infrared Nonlinear Optical Materials

“…Nonlinear optical (NLO) materials have attracted considerable attention due to their potential application in developing new light sources by frequency‐conversion technology [1–27] . In the UV and visible regions, NLO materials have made significant progress.…”