ITS2 Isolation in Endemic Loka Pere Banana: A New Potential DNA Barcode Marker
Mufti Hatur Rahmah,
Fenny Martha Dwivany,
Rizkita R Esyanthi
Abstract:The present research aimed to investigate Loka Pere banana (Musa sp.), an endemic species with distinct characteristics, but currently underrepresented in scientific literature. Our primary goal was to isolate the Internal Transcribed Spacer (ITS2) region from the leaf and corm samples of this unique banana variant. As a methodological approach, we utilized a modified CTAB protocol to extract DNA and amplified the ITS2 region via polymerase chain reaction (PCR) using ITS-S2F and ITS-S3R primers, achieving an a… Show more
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