ITS2: An Ideal DNA Barcode for the Arid Medicinal Plant Rhazya Stricta

Khan, Shahzad; Baeshen, Mohamed N; Ramadan, Hassan A. I.; Baeshen, Nabih A.

doi:10.1007/s40290-019-00266-3

Cited by 12 publications

(11 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Internal transcribed spacer 1 (ITS1) and internal transcribed spacer 2 (ITS2) are the internal regions between 18S and 5.8S, 5.8S, and 28S, respectively. Both have been widely used as molecular markers to explore phylogenetic relationships ( Cheng et al, 2016 ; Khan et al, 2019 ). While, as a promising method, phylogenetic analysis based on the Chloroplast genome (CP) was also applied in our research.…”

Section: Methodsmentioning

confidence: 99%

Modeling of the Potential Geographical Distribution of Three Fritillaria Species Under Climate Change

et al. 2022

View full text Add to dashboard Cite

Fritillaria species, a well-known Chinese traditional medicine for more than 2,000 years, have become rare resources due to excessive harvesting. In order to balance the economical requirement and ecological protection of Fritillaria species, it is necessary to determine (1) the important environmental variables that were responsible for the spatial distribution, (2) distribution change in response to climate change in the future, (3) ecological niche overlap between various Fritillaria species, and (4) the correlation between spatial distribution and phylogenies as well. In this study, the areas with potential ecological suitability for Fritillaria cirrhosa, Fritillaria unibracteata, and Fritillaria przewalskii were predicted using MaxEnt based on the current occurrence records and bioclimatic variables. The result indicated that precipitation and elevation were the most important environmental variables for the three species. Moreover, the current suitable habitats of F. cirrhosa, F. unibracteata, and F. przewalskii encompassed 681,951, 481,607, and 349,199 km2, respectively. Under the scenario of the highest concentration of greenhouse gas emission (SSP585), the whole suitable habitats of F. cirrhosa and F. przewalskii reach the maximum from 2021 to 2100, while those of F. unibracteata reach the maximum from 2021 to 2100 under the scenario of moderate emission (SSP370) from 2021 to 2100. The MaxEnt data were also used to predict the ecological niche overlap, and thus high overlap occurring among three Fritillaria species was observed. The niche overlap of three Fritillaria species was related to the phylogenetic analysis despite the non-significance (P > 0.05), indicating that spatial distribution was one of the factors that contributed to the speciation diversification. Additionally, we predicted species-specific habitats to decrease habitat competition. Overall, the information obtained in this study provided new insight into the potential distribution and ecological niche of three species for the conservation and management in the future.

show abstract

Section: Methodsmentioning

confidence: 99%

Modeling of the Potential Geographical Distribution of Three Fritillaria Species Under Climate Change

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Previous studies have also recorded ITS as an efficient marker for identification of medicinal products to species level (Nazar et al, 2022). Such studies where ITS was utilized are in Iran where it was used to distinguish Ziziphora species; in molecular authentication of the Chinese medicinal species of Liguisticum, usually sold as dried sliced material; and in Saudi Arabia where it was reported as an ideal marker for identifying arid medicinal plants (Khan et al, 2019;Liu et al, 2019;Sheidai et al, 2019). Thus, this study results concur with recommendations by Chen et al, (2010) that ITS2 should be the gold standard barcode for plants identification.…”

Section: Discussionmentioning

confidence: 99%

“…The standardized barcode for plants is a fragment of the plastid gene ribulose 1, 5biphosphate carboxylase gene (rbcL) combined with a fragment of the maturase (matK) gene. The choice for the rbcL in this study is grounded on easy amplification recovery while ITS has high discriminatory ability for closely related species (Khan et al, 2019). The rbcL barcode region is easy to amplify in most of the plant and its sequencing success rate is also high although it is not the most variable one.…”

Section: Introductionmentioning

confidence: 99%

Potential application of DNA barcoding technology for identification of medicinal plant species traded in Nairobi County, Kenya

Mwaura

Ombori

Kamau³

2023

EAJSTI

View full text Add to dashboard Cite

There exists a long history of herbal products use but recently there are concerns on authenticity and safety of some these products. Majority of the herbal products traded in Kenyan markets are processed plant parts, which have been dried or in powder form and are extremely difficult to identify taxonomically. This study was carried out in selected markets in Nairobi County, the capital city of Kenya to assess authenticity of herbal products traded in the region using deoxyribonucleic acid (DNA) barcoding technology by applying internal transcribed spacer (ITS) and ribulose -1,5-bisphosphate carboxylase Large subunit (rbcL) markers. One hundred and twelve herbal products were purchased from practicing herbalists and analyzed using ITS and rbcL DNA barcoding markers. DNA was extracted from acquired samples, amplified and sequences generated. BLAST search with edited generated sequences against the NCBI database was done to recover the expected species. Our findings showed that 35% of the herbal products were wrongly labeled and contained different species compared to those listed on the labels. Out of 92 samples with positive sequences, 58 matched their expected species after the BLAST search. Among the authentic species identified are O. lanceolata, M. oleifera, W. ugandensis, O. europaea, Acacia species, and C. spinarum. However, 32 species did not match the expected species as listed on labels and were concluded to have been substituted. Among them were R. prinoides, T. abyssinica, U. massaica and A. indica, which were substituted with their close relatives, while R. tridentate, Z. usambarense and Prunus africana were substituted with unrelated species with no reported medical benefits. ITS marker had remarkable identification efficiency at 98% to species level compared to rbcL at 89%. We recommend further studies to establish at what stage substitution takes place, as well as utilization of DNA barcoding technology for medicinal plants authentication and verification

show abstract

“…A 2008 review 85 identified 82 publications that used DNA to identify medicinal plants, the majority of studied species came from the Chinese pharmacopeia 101 , 102 , 103 , 104 . More recently, a significant increase in the use of DNA barcodes was seen, as demonstrated by Mishra et al 105 , and several adulterations could be detected in the industry 105 , 106 .…”

Section: Fingerprint Approachesmentioning

confidence: 99%

Quality Control of Herbal Medicines: From Traditional Techniques to State-of-the-art Approaches

et al. 2021

View full text Add to dashboard Cite

Herbal medicines are important options for the treatment of several illnesses. Although their therapeutic applicability has been demonstrated throughout history, several concerns about their safety and efficacy are raised regularly. Quality control of articles of botanical origin, including plant materials, plant extracts, and herbal medicines, remains a challenge. Traditionally, qualitative (e.g., identification and chromatographic profile) and quantitative (e.g., content analyses) markers are applied for this purpose. The compound-oriented approach may stand alone in some cases (e.g., atropine in Atropa belladonna). However, for most plant materials, plant extracts, and herbal medicines, it is not possible to assure quality based only on the content or presence/absence of one (sometimes randomly selected) compound. In this sense, pattern-oriented approaches have been extensively studied, introducing the use of multivariate data analysis on chromatographic/spectroscopic fingerprints. The use of genetic methods for plant material/plant extract authentication has also been proposed. In this study, traditional approaches are reviewed, although the focus is on the applicability of fingerprints for quality control, highlighting the most used approaches, as well as demonstrating their usefulness. The literature review shows that a pattern-oriented approach may be successfully applied to the quality assessment of articles of botanical origin, while also providing directions for a compound-oriented approach and a rational marker selection. These observations indicate that it may be worth considering to include fingerprints and their data analysis in the regulatory framework for herbal medicines concerning quality control since this is the foundation of the holistic view that these complex products demand.

show abstract

ITS2: An Ideal DNA Barcode for the Arid Medicinal Plant Rhazya Stricta

Cited by 12 publications

References 61 publications

Modeling of the Potential Geographical Distribution of Three Fritillaria Species Under Climate Change

Modeling of the Potential Geographical Distribution of Three Fritillaria Species Under Climate Change

Potential application of DNA barcoding technology for identification of medicinal plant species traded in Nairobi County, Kenya

Quality Control of Herbal Medicines: From Traditional Techniques to State-of-the-art Approaches

Contact Info

Product

Resources

About