ITS Regions in Diploids of Aegilops (Poaceae) and their Phylogenetic Implications

Abstract

The nucleotide sequences of the internal transcribed spacer (ITS) of nuclear ribosomal DNA in nine diploid species representing six sections of Aegilops were determined by direct sequencing of PCR-amplified DNA fragments. These sequences were aligned with two ITS sequences of additional species from Genbank. Sequence divergences were estimated using Kimura two-parameter model, and the phylogenetic analyses were performed using the maximum parsimony (MP) and the neighbor-joining (NJ) methods with PAUP and PHYLIP, respectively. The sequence divergences between the diploid species varied from 0.5 %I to 4.68 YO. The resulting MP tree and NJ tree showed relatively congruent phylogenetic relationships among these species, except Ae. cuuduta. Particularly, Ae. speltoides was basal within the two trees. The paraphyletic relationships between Ae. speltoides and two species of Sect. Sitopsis, and between Ae. uniuristutu and two species of Sect. Comopyrum were supported strongly. The ITS data suggest that currently recognized sections within Aegilops should be reconsidered.

Intra‐ and interspecific phylogenetic relationships among diploid Triticum‐Aegilops species (Poaceae) based on base‐pair substitutions, indels, and microsatellites in chloroplast noncoding sequences

“…Kimber et al (1983) also found no preferential pairing between the two genomes. Wang et al (2000) claimed that the classification of section Comopyrum should be reconsidered based on their study of ITS sequences. Badaeva et al (1996a, b) also postulated that Ae.…”

“…Later, many cytoplasmic and cytological studies (e.g., Tsunewaki et al, 1976;Teoh and Hutchinson, 1983) and an isozyme analysis (e.g., Benito et al, 1987) were done to reveal the genome relationships of Triticum-Aegilops species. More recent analyses have been focused on molecular markers in nuclei (Dvořák and Zhang, 1992;Sasanuma et al, 1996;Wang et al, 2000;Huang et al, 2002a, b;Sallares and Brown, 2004) or organelles (Tsunewaki and Ogihara, 1983;Terachi and Tsunewaki, 1986;Ogihara and Tsunewaki, 1988;Terachi and Tsunewaki, 1992). However, questions remain about the taxonomic relationships between Triticum-Aegilops sections and species.…”

Classification of 17 species Aegilops using DNA barcoding and SNPs, reveals gene flow among Aegilops biuncialis, Aegilops juvenalis, and Aegilops columnaris

“…DNA barcoding is applied in many fields ( Pečnikar and Buzan, 2014 ), and allows animal, plant, and fungal species to be rapidly identified using one or several standard DNA sequences. Previous DNA barcoding studies of Aegilops species mainly sought to identify species that are difficult to distinguish morphologically ( Wang et al., 2000 ; Goryunova et al., 2005 ; Giraldo et al., 2016 ; Dizkirici et al., 2016 ), and phylogenetic analysis of Aegilops and Triticum species using DNA barcodes ( Yamane and Kawahara, 2005 ; Petersen et al., 2006 ; Meimberg et al., 2009 ). However, no studies have applied universal DNA barcodes to identify all species within the Aegilops genus.…”

Genetic diversity assessment of aegilops germplasm by RAPD molecular markers

“…many cytoplasmic and cytological studies (e.g., Tsunewaki et al, 1976;Teoh and Hutchinson, 1983) and an isozyme analysis (e.g., Benito et al, 1987) were done to reveal the genome relationships of Triticum-Aegilops species. More recent analyses have been focused on molecular markers in nuclei (Dvo ák and Zhang, 1992;Sasanuma et al, 1996Wang et al, 2000Huang et al, 2002a, b;Sallares and Brown, 2004) or organelles (Tsunewaki and Ogihara, 1983;Terachi and Tsunewaki, 1986;Ogihara and Tsunewaki, 1988;Terachi and Tsunewaki, 1992).…”