ITS 2 sequences heterogeneity in Phlebotomus sergenti and Phlebotomus similis (Diptera, Psychodidae): possible consequences in their ability to transmit Leishmania tropica

Depaquit, Jérôme; Ferté, Hubert; Léger, N.; Lefranc, Fabienne; Alves-Pires, C.; Hanafi, Hanafi; Maroli, M.; Morillas‐Márquez, F.; Rioux, J. A.; Svobodová, Milena; Volf, Petr

doi:10.1016/s0020-7519(02)00088-7

Cited by 102 publications

(86 citation statements)

References 28 publications

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“…This species, a proven vector of CL caused by L. tropica, is recorded in many countries of the Mediterranean basin including western Europe and northern Africa. Its migration from Central Asia and subsequent colonization of the Mediterranean region has been hypothesized [16]. In Italy, P. sergenti species had been reported in the past only from a few places of Catania area [2,17].…”

Section: Discussionmentioning

confidence: 99%

Persistence of phlebotomine Leishmania vectors in urban sites of Catania (Sicily, Italy)

Lisi

D¿Urso

Vaccalluzzo

et al. 2014

Parasites & Vectors

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Background: Pioneering research on "Mediterranean Kala-Azar" carried out by Adler and Theodor early in the past century (~1930s) had identified Catania city (Sicily) as a major focus of the disease nowadays known as zoonotic visceral leishmaniasis (VL). Despite the fact that disease in both humans and dogs has continued to be highly prevalent in the Catania province up to the present times, research on Leishmania vectors in this urban focus dates back to that distant period. This study aimed to evaluate the persistence and current composition of the sand fly fauna in urban environments of Catania in recent years, 2006 and 2013. Methods: In 2006 fifty-one suitable collecting sites were identified within 44 sub-units of a grid drawn to include the urban Catania area. In 2013 the survey was restricted to four of the most productive and representative sites resulting from the 2006 survey. In both periods 3 collections per month were performed using standard sticky traps set for 3 days in wall holes/cavities along public roads, from the end of April through December. Results: 43/51 sites (84.3%) were found positive for sand flies. The 2006 collections accounted for a total of 4341 specimens including six species. Among competent Leishmania vector species, P. perniciosus was the most prevalent (36.5%) being identified in all sand fly-positive sites, with significant abundance in those of the old city centre. Other species of interest were P. sergenti (2.5%) and P. neglectus (1.5%). The 2013 survey produced 1130 sand flies, of which 39.5% were P. perniciosus, 1.6% P. sergenti and 0.7% P. neglectus. A search for Leishmania DNA in a small sample of 72 P. perniciosus females revealed 11% infection prevalence. Conclusions: Our findings from an old urban focus of leishmaniasis demonstrate that phlebotomine sand flies have adapted fairly well to the drastic environmental changes that have occurred in cities of the Western world in the past century and still represent a potential risk for Leishmania transmission.

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Section: Discussionmentioning

confidence: 99%

Persistence of phlebotomine Leishmania vectors in urban sites of Catania (Sicily, Italy)

Lisi

D¿Urso

Vaccalluzzo

et al. 2014

Parasites & Vectors

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“…As a coding gene, it was easier to sequence than rDNA ITS2, also informative at this taxonomic level (Depaquit et al 2002), which includes, in the case of Transphlebotomus, several poly-A and poly-T sequences.…”

Section: Methodsmentioning

confidence: 99%

A molecular analysis of the subgenus Transphlebotomus Artemiev, 1984 (Phlebotomus, Diptera, Psychodidae) inferred from ND4 mtDNA with new northern records of Phlebotomus mascittii Grassi, 1908

et al. 2004

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A comparative molecular study was performed on the three species of Phlebotomine sandflies belonging to the subgenus Transphlebotomus Artemiev whose morphological differentiation is difficult. All three species have been suspected, but never proven, to be vectors of Leishmania infantum. The ND4 gene from mtDNA was sequenced from specimens of five populations of Phlebotomus mascittii Grassi from Belgium (the first records of Phlebotomine sandflies from this country), France, and Germany. Additionally, specimens from one population of Lebanese P. canaaniticus Adler and Theodor and of one population of Cypriot P. economidesi Leger, Depaquit and Ferte topotypes were examined. The results reveal the validity of the three taxa inferred from molecular variation and a molecular homogeneity shared by all of the populations of P. mascittii included into the present study. The epidemiological consequences are discussed.

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“…This species has often been misidentified as P. sergenti, sister species of P. similis, implying a similar vector capability in relation to Leishmania species. 55 This species is believed to be the potential vector of L. tropica in Crete, 17 a fact that needs further investigation. P. similis was found in the Ionian Islands and Crete where CL is widespread but also in areas on the coast of the Aegean Sea (Figures 1 and 3) where CL cases have never been reported.…”

Section: Rodos Islandmentioning

confidence: 98%

Leishmaniases in Greece

Ntais¹,

Sifaki-Pistola²,

Christodoulou³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. During the last 35 years, visceral leishmaniasis has spread in Greece with autochthonous human cases appearing in 41 of the 54 prefectures. The occurrence of the disease was mapped and related to dog seropositivity, environmental and geospatial risk factors. Average dog seropositivity was 22.1% and positive animals were found in 43 of 54 prefectures. Factors like: altitude, presence of water bodies, land use, wind speed, mean land surface temperature, mean relative humidity, and mean annual rainfall were found to affect dog seropositivity. Cases of cutaneous leishmaniasis, caused by Leishmania tropica are also increasing. Phlebotomus similis believed to be the potential vector of L.

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ITS 2 sequences heterogeneity in Phlebotomus sergenti and Phlebotomus similis (Diptera, Psychodidae): possible consequences in their ability to transmit Leishmania tropica

Cited by 102 publications

References 28 publications

Persistence of phlebotomine Leishmania vectors in urban sites of Catania (Sicily, Italy)

Persistence of phlebotomine Leishmania vectors in urban sites of Catania (Sicily, Italy)

A molecular analysis of the subgenus Transphlebotomus Artemiev, 1984 (Phlebotomus, Diptera, Psychodidae) inferred from ND4 mtDNA with new northern records of Phlebotomus mascittii Grassi, 1908

Leishmaniases in Greece

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