iTRAQ technology-based identification of human peripheral serum proteins associated with depression

Wang, Q; Su, Xiaojie; Jiang, Xiaoming; Dong, Xin Qi; Yang, Fan; Zhang, J; Yu, Chia‐Jung; Gao, Weimin; Shi, Shanshan; Jiang, Jian‐Dong; Jiang, Wen-An; Wei, Taiming

doi:10.1016/j.neuroscience.2016.05.055

Cited by 40 publications

(28 citation statements)

References 111 publications

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“…Additional evidence has recently begun to accumulate indicating that there may be an association between SAA and depressive symptoms. While early studies of elderly patients and youth with diabetes type I and II did not find any significant relation between SAA and depression, a new report found significantly elevated SAA levels in the serum of unmedicated depressed patients (selected as those scoring higher than 17 on HAMD‐17) . Furthermore, a recent large population‐based study found that plasma SAA levels were increased in patients with depressive disorders .…”

Section: Discussionmentioning

confidence: 97%

“…For example, patients with a diagnosis of major depressive disorder (MDD) as well as suicide attempters have been identified to have higher levels of inflammatory markers than healthy controls . Frequently, the presence of somatic or psychiatric comorbidities are listed as exclusion criteria in psychiatric biomarker studies, and healthy controls are often required to be completely free of all morbidities and medications . It is not known whether biomarkers identified in such carefully pruned populations would be useable in the everyday clinical setting that see patients with all types of psychiatric and somatic comorbidities.…”

Section: Introductionmentioning

confidence: 99%

“…Serum amyloid A (SAA), like CRP, is an acute‐phase plasma protein, synthesized predominantly by the liver and induced by IL‐1β and IL‐6 . SAA has been implicated in several chronic inflammatory diseases, such as rheumatoid arthritis and cardiovascular disease , and a recent study reported elevated levels of SAA in the plasma of patients with clinical depression relative to healthy controls . That study was carried out in a pure population of subjects without any somatic diseases, psychiatric comorbidities, or medication use.…”

Section: Introductionmentioning

confidence: 99%

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The acute‐phase mediator serum amyloid A is associated with symptoms of depression and fatigue

Bryleva

Keaton

Grit

et al. 2017

Acta Psychiatr Scand

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The acute‐phase mediator serum amyloid A is associated with symptoms of depression and fatigue

Bryleva

Keaton

Grit

et al. 2017

Acta Psychiatr Scand

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“…A coupled iTRAQ/LC–MS/MS-based approach has been applied as a powerful sensitive proteomics tool for concurrently quantifying proteins in 4- or 8-plex samples during the discovery of disease biomarkers [21]. However, to the best of our knowledge, there is no consequential study on the use of iTRAQ-based approaches in the identification of serum biomarkers for IPF.…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis

et al. 2017

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Idiopathic pulmonary fibrosis (IPF) is a gradual lung disease with a survival of less than 5 years post-diagnosis for most patients. Poor molecular description of IPF has led to unsatisfactory interpretation of the pathogenesis of this disease, resulting in the lack of successful treatments. The objective of this study was to discover novel noninvasive biomarkers for the diagnosis of IPF. We employed a coupled isobaric tag for relative and absolute quantitation (iTRAQ)-liquid chromatography–tandem mass spectrometry (LC–MS/MS) approach to examine protein expression in patients with IPF. A total of 97 differentially expressed proteins (38 upregulated proteins and 59 downregulated proteins) were identified in the serum of IPF patients. Using String software, a regulatory network containing 87 nodes and 244 edges was built, and the functional enrichment showed that differentially expressed proteins were predominantly involved in protein activation cascade, regulation of response to wounding and extracellular components. A set of three most significantly upregulated proteins (HBB, CRP and SERPINA1) and four most significantly downregulated proteins (APOA2, AHSG, KNG1 and AMBP) were selected for validation in an independent cohort of IPF and other lung diseases using ELISA test. The results confirmed the iTRAQ profiling results and AHSG, AMBP, CRP and KNG1 were found as specific IPF biomarkers. ROC analysis indicated the diagnosis potential of the validated biomarkers. The findings of this study will contribute in understanding the pathogenesis of IPF and facilitate the development of therapeutic targets.

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“…Differential proteomics relies on iTRAQ technology and can reflect the regulatory mechanisms associated with pathological conditions. This approach may be employed in a wide variety of disorders, including cancer, cardiovascular disease and psychiatric illness (16)(17)(18)(19). Proteomic profiling has revealed that considerable pathophysiological changes, including iTRAQ-based quantitative proteomics analysis of the potential application of secretoneurin gene therapy for cardiac hypertrophy induced by DL-isoproterenol hydrochloride in mice altered energy metabolism, enhanced protein synthesis, proto-oncogene expression, elevated oxidative stress, occur during cardiac hypertrophy (20)(21)(22).…”

Section: Introductionmentioning

confidence: 99%

iTRAQ‑based quantitative proteomics analysis of the potential application of secretoneurin gene therapy for cardiac hypertrophy induced by DL‑isoproterenol hydrochloride in mice

Chen

Jiang

et al. 2020

Int J Mol Med

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A previous study by our group demonstrated a protective role of the neuropeptide secretoneurin (SN) in dL-isoproterenol hydrochloride (ISO)-induced cardiac hypertrophy in mice. To further characterize the molecular mechanism of SN treatment, an isobaric tags for relative and absolute quantification (iTRAQ)-based quantitative proteomic analysis was applied to identify putative target proteins and molecular pathways. An SN expression vector was injected into the myocardial tissues of mice, and the animals were then subcutaneously injected with ISO (5 mg/kg/day) for 7 days to induce cardiac hypertrophy. The results of echocardiography and hemodynamic measurements indicated that the function of the heart impaired by ISO treatment was significantly ameliorated via SN gene injection. The investigation of heart proteomics was performed by iTRAQ-based liquid chromatography-tandem mass spectrometry analysis. A total of 2,044 quantified proteins and 15 differentially expressed proteins were associated with SN overexpression in mice with cardiac hypertrophy. Functional enrichment analysis demonstrated that these effects were possibly associated with metabolic processes. A protein-protein interaction network analysis was constructed and the data indicated that apolipoprotein c-III (Apoc3) was associated with the positive effect of SN on the induction of cardiac hypertrophy in mice. The present study proposed a potential mechanism of SN action on Apoc3 upregulation that may contribute to the amelioration of cardiac hypertrophy. These findings can aid the clinical application of SN in patients with cardiac hypertrophy.

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iTRAQ technology-based identification of human peripheral serum proteins associated with depression

Cited by 40 publications

References 111 publications

The acute‐phase mediator serum amyloid A is associated with symptoms of depression and fatigue

The acute‐phase mediator serum amyloid A is associated with symptoms of depression and fatigue

iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis

iTRAQ‑based quantitative proteomics analysis of the potential application of secretoneurin gene therapy for cardiac hypertrophy induced by DL‑isoproterenol hydrochloride in mice

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