iTRAQ quantitatively proteomic analysis of the hippocampus in a rat model of accumulative microwave-induced cognitive impairment

Wang, Hui; Tan, Shengzhi; Dong, Ji; Zhang, Jing; Yao, Baojin; Xu, Xinping; Hao, Yanhui; Yu, Chao; Zhou, Hongmei; Zhao, Li; Peng, Ruiyun

doi:10.1007/s11356-019-04873-0

Cited by 10 publications

(10 citation statements)

References 35 publications

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“…Microwaves bring convenience but also trigger discussions about the negative effects, especially hazards of complex electromagnetic environments. The World Health Organization (WHO) regard electromagnetic waves as the fourth pollutant, and the International Agency for Research on Cancer (IARC) also classified electromagnetic waves as "possibly carcinogenic to humans" (Group 2B) 4 .The central nervous system is considered one of the most sensitive targets for microwave exposure [5][6][7] . Previous studies have found that microwaves can induce certain damage, such as impairment of memory functions, inhibition of brain electrical activity, and degeneration of hippocampal tissue structure [8][9][10] .…”

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confidence: 99%

“…As we live in a complex electromagnetic environment, humans are continuously exposed to multiple electromagnetic waves of various frequencies. However, most studies have focused on the effects caused by single-band microwaves, and only a few researchers have been concerned with the hazards from multi-frequency microwave radiation 6,11,12 . L-and C-band microwaves are commonly used for communication in daily life, such as weather satellites 13 , and some synthetic aperture radars 14,15 , etc.…”

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confidence: 99%

“…The central nervous system is considered one of the most sensitive targets for microwave exposure [5][6][7] . Previous studies have found that microwaves can induce certain damage, such as impairment of memory functions, inhibition of brain electrical activity, and degeneration of hippocampal tissue structure [8][9][10] .…”

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confidence: 99%

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Effects of 1.5 and 4.3 GHz microwave radiation on cognitive function and hippocampal tissue structure in Wistar rats

Zhu

Wang

et al. 2021

Sci Rep

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Previous studies have shown that single-frequency microwave radiation can lead to cognitive decline in rats. However, few studies have focused on the combined effects of irradiation with different frequencies of microwaves. Our research aimed to investigate the effects of 1.5 GHz and 4.3 GHz microwave radiation, singly and in combination, on cognitive function and hippocampal tissue structure in rats. A total of 140 male Wistar rats were randomly divided into 4 groups: the S group (sham radiation group), L10 group (10 mW/cm2 1.5 GHz group), C10 group (10 mW/cm2 4.3 GHz band group) and LC10 group (10 mW/cm2 1.5 and 4.3 GHz multi-frequency radiation group). For 1–28 days after microwave radiation, we analyzed the average escape latency for the Morris water maze task, electroencephalograms, change in hippocampal tissue structure and ultrastructure, content of the Nissl body in the hippocampus, and activities of lactate dehydrogenase and succinate dehydrogenase. Compared to the S group, all exposure groups showed varying degrees of learning and memory decline and hippocampal structural damage. The results showed that 1.5 GHz and 4.3 GHz microwave radiation was able to induce cognitive impairment and hippocampal tissue damage in rats and combined radiation with both frequencies caused more serious injuries, but none of these damaging effects varied with microwave frequency.

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confidence: 99%

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confidence: 99%

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Effects of 1.5 and 4.3 GHz microwave radiation on cognitive function and hippocampal tissue structure in Wistar rats

Zhu

Wang

et al. 2021

Sci Rep

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“…In the process of heating, the protein is prone to denaturation and produce carcinogens [ 75 ]. Several studies have demonstrated that exposure to microwave radiation can cause nerve damage in humans [ 76 ], as well as oxidative stress and inflammation [ 77 ]. However, microwave radiation appears to have little if any adverse effects on plant proteins; in fact, one study was found that microwave radiation destroyed harmful proteins and improved some properties of proteins [ 78 ].…”

Section: Transformation Of Natural Productsmentioning

confidence: 99%

Microwave technology: a novel approach to the transformation of natural metabolites

Fang

et al. 2021

Chin Med

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Microwave technology is used throughout the world to generate heat using energy from the microwave range of the electromagnetic spectrum. It is characterized by uniform energy transfer, low energy consumption, and rapid heating which preserves much of the nutritional value in food products. Microwave technology is widely used to process food such as drying, because food and medicinal plants are the same organisms. Microwave technology is also used to process and extract parts of plants for medicinal purposes; however, the special principle of microwave radiation provide energy to reaction for transforming chemical components, creating a variety of compounds through oxidation, hydrolysis, rearrangement, esterification, condensation and other reactions that transform original components into new ones. In this paper, the principles, influencing factors of microwave technology, and the transformation of natural metabolites using microwave technology are reviewed, with an aim to provide a theoretical basis for the further study of microwave technology in the processing of medicinal materials.

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“…Moreover, the National Toxicology Program of USA have reported the latest evidence that microwave radiation at a frequency of 900MHz can cause cancers such as glioma in rats [1]. The brain is considered to be sensitive to microwave radiation, as many studies have reported the damaging effects of microwave exposure on the brain [2][3][4][5]. Therefore, it is important to study the biological effects and underlying mechanisms of microwave-induced neuronal damage.…”

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MiR-30a-5p Downregulation Induces Neuronal Autophagy by Activating AMPK After 2856MHz Microwave Radiation

Hao

Wang

et al. 2021

Preprint

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Background With the development of science and technology, microwaves are being widely used. More and more attention has been paid to the potential health hazards of microwave exposure. The regulation of miR-30a-5p (miR-30a) on autophagy is involved in the pathophysiological process of many diseases. Our previous study found that 30 mW/cm2 microwave radiation could reduce miR-30a expression and activate neuronal autophagy in rat hippocampus. However, the roles played by miR-30a in microwave-induced neuronal autophagy and related mechanisms remain largely unexplored. Results In the present study, we established neuronal damage models by exposing rat hippocampal neurons and rat adrenal pheochromocytoma (PC12) cell-derived neuron-like cells to 30 mW/cm2 microwave, which resulted in miR-30a downregulation and autophagy activation in vivo and in vitro. Bioinformatics analysis was conducted, and Beclin1, Prkaa2, Irs1, Pik3r2, Rras2, Ddit4, Gabarapl2 and autophagy-related gene 12 (Atg12) were identified as potential downstream target genes of miR-30a involved in regulating autophagy. Based on our previous findings that microwave radiation can cause a neuronal energy metabolism disorder, Prkaa2, encoding adenosine 5’-monophosphate-activated protein kinase α2 (AMPKα2, an important catalytic subunit of energy sensor AMPK), was selected for further analysis. Dual-luciferase reporter assay results showed that Prkaa2 is a downstream target gene of miR-30a. Microwave radiation increased the expression and phosphorylation (Thr172) of AMPKα both in vivo and in vitro. Moreover, the transduction of cells with miR-30a mimics suppressed AMPKα2 expression, inhibited AMPKα (Thr172) phosphorylation and reduced autophagy flux in neuron-like cells. Importantly, miR-30a mimics abolished microwave-activated autophagy and inhibited microwave-induced AMPKα (Thr172) phosphorylation. Conclusions AMPKα2 was a newly founded downstream gene of miR-30a involved in autophagy regulation, and miR-30a downregulation after microwave radiation could promote neuronal autophagy by increasing AMPKα2 expression and activating AMPK signaling.

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iTRAQ quantitatively proteomic analysis of the hippocampus in a rat model of accumulative microwave-induced cognitive impairment

Cited by 10 publications

References 35 publications

Effects of 1.5 and 4.3 GHz microwave radiation on cognitive function and hippocampal tissue structure in Wistar rats

Effects of 1.5 and 4.3 GHz microwave radiation on cognitive function and hippocampal tissue structure in Wistar rats

Microwave technology: a novel approach to the transformation of natural metabolites

MiR-30a-5p Downregulation Induces Neuronal Autophagy by Activating AMPK After 2856MHz Microwave Radiation

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