iTRAQ proteome analysis reflects a progressed differentiation state of epiblast derived versus inner cell mass derived murine embryonic stem cells

Competence to epithelialise coincides with competence to differentiate in pluripotent cells

“…We show Itga3 is upregulated very early, prior to irreversible exit from naive pluripotency, in vitro and are also upregulated during peri-implantation development, in keeping with previous studies (Sutherland et al, 1993;Hayashi et al, 2011;Mohammed et al, 2017). However it is unlikely that these are the only changes driving epithelialisation: for example proteomic (Fröhlich et al, 2013) and transcriptomic (Hayashi et al, 2011) studies reveal that a broad set of actin-regulators and intermediate filaments are upregulated transition from naive to primed states in vitro.…”

Cdx2Efficiently Induces Trophoblast Stem-Like Cells in Naïve, but Not Primed, Pluripotent Stem Cells

“…Interestingly, ESC-derived TSClike cells fail to acquire genomic methylation features of TSCs [21], arguing that epigenetic differences between ESCs and EpiSCs could limit the developmental potential of EpiSCs to become TSCs. Differences in the transcriptomes, proteomes, and chromatin states of ESCs and EpiSCs have been compared [62][63][64][65]. Notably, NuRD-dependent methylation prevents ESCs from spontaneously acquiring trophoblast fate [66][67][68][69].…”

Transcriptomic and proteomic analyses of splenic immune mechanisms of rainbow trout (Oncorhynchus mykiss) infected by Aeromonas salmonicida subsp. salmonicida

“…Aeromonas hydrophila, 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) and Spiroplasma eriocheiris, in order to identify the pathogen responsive proteins [19][20][21]. Furthermore, a series of studies in diverse fields was conducted based on the iTRAQ method [22][23][24][25][26].…”