iTRAQ-Based Quantitative Proteomics Suggests Synaptic Mitochondrial Dysfunction in the Hippocampus of Rats Susceptible to Chronic Mild Stress

Xie, Hui; Huang, Haojun; Tang, Min; Wu, Yan; Huang, Rongzhong; Liu, Zhao; Zhou, Mei; Liao, Wei; Zhou, Jian

doi:10.1007/s11064-018-2664-y

Cited by 18 publications

(14 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We first performed the SPT and FST analysis to evaluate the depression-like behavior of CMS-exposed rats, thereby obtaining the depression-susceptible subgroup 33 . Through an eight-week CMS regime, rats displaying a significant reduction in sucrose preference were considered to be susceptible to CMS-induced depression 33 . As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…To gain insights into the involvement of these proteins in different biological functions and pathways, these differentially expressed proteins in each CMS response group were analyzed with the OmicsBean tool 33,34 . GO analysis of the 136 differentially expressed proteins from the depression-susceptible group identified 768, 203, 222, and 33 terms to be significantly enriched ( p < 0.05) in the biological process (BP), molecular function (MF), cellular component (CC), and KEGG pathway categories respectively (Supplementary Table S3).…”

Section: Resultsmentioning

confidence: 99%

“…The CMS procedure has been suggested to mimic socio-environmental stresses in inducing depression- and anxiety-related behaviors 7,10,33,47,48 . In our study, we identified three different stress-response phenotypes in the CMS rat model, mainly assessed by means of SPT, FST, and EMT 7,10,33 . Some rats exposed to CMS exhibited anhedonia-like behavior where their sucrose preferences were slower compared with the control.…”

Section: Discussionmentioning

confidence: 99%

“…A multi-omics tool (OmicsBean) (http://www.omicsbean.cn) was employed for in silico analysis of the identified differentially expressed proteins 33 . The enrichment analyses of these proteins in biological process (BP), cellular component (CC), and molecular function (MF) were conducted based on the Gene Ontology (GO) terms of each protein.…”

Section: Methodsmentioning

confidence: 99%

“…Further, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was employed and the pathways or GO terms with p -value <0.05 were considered significant 34 . Additionally, evaluation of protein–protein interactions was conducted with STRING database and visualized using Cytoscape software, as described previously 33 .…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress

Tang

Huang

et al. 2019

Transl Psychiatry

Self Cite

View full text Add to dashboard Cite

Chronic stressful occurrences are documented as a vital cause of both depression and anxiety disorders. However, the stress-induced molecular mechanisms underlying the common and distinct pathophysiology of these disorders remains largely unclear. We utilized a chronic mild stress (CMS) rat model to differentiate and subgroup depression-susceptible, anxiety-susceptible, and insusceptible rats. The hippocampus was analyzed for differential proteomes by combining mass spectrometry and the isobaric tags for relative and absolute quantitation (iTRAQ) labeling technique. Out of 2593 quantified proteins, 367 were aberrantly expressed. These hippocampal protein candidates might be associated with susceptibility to stress-induced depression or anxiety and stress resilience. They provide the potential protein systems involved in various metabolic pathways as novel investigative protein targets. Further, independent immunoblot analysis identified changes in Por, Idh2 and Esd; Glo1, G6pdx, Aldh2, and Dld; Dlat, Ogdhl, Anxal, Tpp2, and Sdha that were specifically associated to depression-susceptible, anxiety-susceptible, or insusceptible groups respectively, suggesting that identical CMS differently impacted the mitochondrial and metabolic processes in the hippocampus. Collectively, the observed alterations to protein abundance profiles of the hippocampus provided significant and novel insights into the stress regulation mechanism in a CMS rat model. This might serve as the molecular basis for further studies that would contributed to a better understanding of the similarities and differences in pathophysiologic mechanisms underlying stress-induced depression or anxiety, and stress resiliency.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress

Tang

Huang

et al. 2019

Transl Psychiatry

Self Cite

View full text Add to dashboard Cite

show abstract

Dopaminergic signaling in prefrontal cortex contributes to the antidepressant effect of electroacupuncture: An iTRAQ‐based proteomics analysis in a rat model of CUMS

Zhang

et al. 2021

The Anatomical Record

View full text Add to dashboard Cite

Electroacupuncture (EA) is used as an adjunctive treatment for depression.This study was conducted to evaluate the efficacy and mechanisms of EA in the depressive rat model induced by chronic unpredictable mild stress (CUMS) in male adult Wistar rats. The underlying mechanisms were explored by using isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis of the proteins in the prefrontal cortex (PFC), and observing the number of the PFC neurons stained with hematoxylin and eosin (H&E) and synaptic morphological changes under transmission electron microscopy (TEM). The results showed that EA plus paroxetine (EA + Par) for 1 week significantly relieved depression-like anhedonia symptoms and improved anxiety-like behavior, accompanied by the improvements in synaptic morphology and a significant increase of PFC neurons. Moreover, EA or paroxetine alone significantly alleviated anhedonia symptoms after 2 weeks of intervention. Additionally, iTRAQ analysis showed that dopaminergic signaling was significantly altered in CUMS rats after 1 week of EA treatment. As the critical enzyme of this pathway, aromatic-L-amino-acid decarboxylase (DDC) was significantly upregulated after the treatment with EA + Par for 1 week. These findings

show abstract

Beneficial effects of vitamin D on anxiety and depression-like behaviors induced by unpredictable chronic mild stress by suppression of brain oxidative stress and neuroinflammation in rats

Bakhtiari-Dovvombaygi

Izadi

Moghaddam

et al. 2020

Naunyn-Schmiedeberg's Arch Pharmacol

View full text Add to dashboard Cite

iTRAQ-Based Quantitative Proteomics Suggests Synaptic Mitochondrial Dysfunction in the Hippocampus of Rats Susceptible to Chronic Mild Stress

Cited by 18 publications

References 43 publications

Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress

Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress

Dopaminergic signaling in prefrontal cortex contributes to the antidepressant effect of electroacupuncture: An iTRAQ‐based proteomics analysis in a rat model of CUMS

Beneficial effects of vitamin D on anxiety and depression-like behaviors induced by unpredictable chronic mild stress by suppression of brain oxidative stress and neuroinflammation in rats

Contact Info

Product

Resources

About