iTRAQ-based Quantitative Proteomics Study in Patients with Refractory &lt;i&gt;Mycoplasma pneumoniae&lt;/i&gt; Pneumonia

Yu, Jialu; Song, Qibin; Xie, Zhiwei; Jiang, Wenhui; Chen, Jiahui; Fan, Huifeng; Xie, Ying; Liu, Gen

doi:10.7883/yoken.jjid.2016.355

Cited by 19 publications

(23 citation statements)

References 35 publications

(33 reference statements)

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“…Our results suggested that patients who developed atelectasis had longer fever duration, higher serum CRP and LDH levels, larger area of lung consolidation (> 2/3 lobe), and more frequent intraluminal obstruction with mucus plugs and mucosal necrotic exudate. Serum CRP and LDH are important in ammatory markers especially in pediatric RMPP [2,6,[15][16][17][18] . Longer fever duration, higher CRP and LDH levels in group A suggested more excessive in ammatory response, which further suggested these patients need timely higher dose corticosteroid treatment.…”

Section: Discussionmentioning

confidence: 99%

Unresolved atelectasis in refractory Mycoplasma pneumoniae pneumonia: Predictive factors and the influence of corticosteroids and bronchoscopy lavage therapy

Liu

Wang³

et al. 2020

Preprint

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Background Unresolved atelectasis occurs in some children with refractory mycoplasma pneumoniae pneumonia (RMPP). The aim was to analyze factors predicting unresolved atelectasis in RMPP and the impact of corticosteroids and bronchoscopy lavage therapy (BLT) on developing atelectasis. MethodsWe retrospectively analyzed data for 230 pediatric RMPP from January 2013 to June 2017 in Beijing Children's Hospital. In this study, we diagnosed RMPP when patients' clinical and radiological findings deteriorated after 7 days of macrolide therapy, peripheral blood C-reactive protein (CRP) was higher than 40mg/L, and chest imaging showed consolidation with high density > 1/2 pulmonary lobe.We divided patients into two groups according to the presence/absence of atelectasis on chest imaging after a 6-month follow-up. We calculated the predictive value of fever duration, levels of CRP and lactate dehydrogenase (LDH), and the size of lobe consolidation, regarding atelectasis.Additionally, we compared the starting time and dosage of corticosteroids and the starting time of BLT between the two groups.Results Ninety-five patients developed atelectasis (atelectasis group/group A), and 135 patients did not (non-atelectasis group/group NA). Chest imaging showed > 2/3 pulmonary lobe consolidation in 93.7% of patients in group A and 54.1% of patients in group NA. Multiple logistic regression analysis showed that fever duration, CRP and LDH levels, and lobe consolidation were related to developing atelectasis. Areas under the curve revealed that CRP ≥ 137 mg/L had 82.11% sensitivity and 80.07% specificity, and LDH ≥ 471 IU/L had 62.65% sensitivity and 60.31% specificity to predict atelectasis.Fewer patients receiving corticosteroids and BLT within 10 days after illness onset developed atelectasis.Conclusions Fever duration>10 days, CRP and LDH levels, and lobe consolidation are risk factors for developing atelectasis in RMPP. CRP ≥ 137 mg/L, LDH ≥ 471 IU/L, and >2/3 pulmonary lobe consolidation were significant predictors of atelectasis, which can aid in early recognition.Corticosteroid administration and subsequent BLT within 10 days of the disease onset, and increased corticosteroid dosage may help reduce the incidence of atelectasis in these RMPP patients.

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Section: Discussionmentioning

confidence: 99%

Unresolved atelectasis in refractory Mycoplasma pneumoniae pneumonia: Predictive factors and the influence of corticosteroids and bronchoscopy lavage therapy

Liu

Wang³

et al. 2020

Preprint

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“…Although cord blood serum can be richer in intracellular proteins and more varied than matured serum, the study was conducted with older MS instrumentation (Applied Biosystems, QSTAR‐Pulsar) and they reported quantification of in the order of 200 proteins. Other studies of respiratory afflictions have included tuberculosis and Mycoplasma pneumoniae (MP) infections (see below). Examples of these applications are indicated in Table A.…”

Section: Applications Of Isobaric Labeling For Serum and Plasma Protementioning

confidence: 99%

“…While the latter studies were performed using ESI (AB Sciex 5600), Li et al used MALDI‐TOF/TOF (AB Sciex 5800) and iTRAQ to profile the serum of PTB patients, comparing sera from drug resistant, smear‐positive and smear‐negative pulmonary tuberculosis cases with normal controls and pneumonia patients (87 samples in iTRAQ discovery and 207 in the ELISA validations) and found that sex hormone binding globulin (SHBG) was significantly elevated in PTB . Also considering bacterial infection and respiratory afflictions, Yu et al compared serum from healthy controls and patients with mycoplasma pneumoniae pneumonia …”

Section: Applications Of Isobaric Labeling For Serum and Plasma Protementioning

confidence: 99%

“…137,138,171 Included in these was the use of a pooled iTRAQ strategy to compare treated, untreated and cured PTB patients with healthy controls (250 samples in total), in which markers of cured PTB were identified. 171 While the latter studies were performed using ESI 139 Cao and co-workers used iTRAQ labeling to analyze plasma from sepsis patients, including ten 4-plex iTRAQ comparisons of infected and uninfected subjects (n = 39 in total). These ESI data (Orbitrap-Velos) indicated age related differences in the incidence of infection.…”

Section: Infectionmentioning

confidence: 99%

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Analysis of the plasma proteome using iTRAQ and TMT‐based Isobaric labeling

Moulder

Bhosale

Goodlett

et al. 2017

Mass Spectrometry Reviews

122

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Over the past decade, chemical labeling with isobaric tandem mass tags, such as isobaric tags for relative and absolute quantification reagents (iTRAQ) and tandem mass tag (TMT) reagents, has been employed in a wide range of different clinically orientated serum and plasma proteomics studies. In this review the scope of these works is presented with attention to the areas of research, methods employed and performance limitations. These applications have covered a wide range of diseases, disorders and infections, and have implemented a variety of different preparative and mass spectrometric approaches. In contrast to earlier works, which struggled to quantify more than a few hundred proteins, increasingly these studies have provided deeper insight into the plasma proteome extending the numbers of quantified proteins to over a thousand.

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“…Increasing numbers of refractory or severe Mycoplasma pneumoniae pneumonia (MPP) cases have been reported worldwide, especially in Asia [2,3] . Previous studies have shown that refractory Mycoplasma pneumoniae pneumonia (RMPP) is associated with prolonged fever, high levels of C-reactive protein (CRP), airway hypersecretion and consolidation on chest imaging [4,5] . It has been con rmed that the excessive immune response of the host plays an important role in the development of RMPP [5,6] .…”

Section: Introductionmentioning

confidence: 99%

Impact of viral coinfection and macrolide-resistant mycoplasma infection in children with refractory Mycoplasma pneumoniae pneumonia

Zhou

Wang

Chen

et al. 2020

Preprint

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Background: Cases of refractory Mycoplasma pneumoniae pneumonia have been increasing recently; however, whether viral coinfection or macrolide-resistant M. infection contribute to the development of refractory M. pneumoniae pneumonia remains unclear. This study aimed to investigate the impacts of viral coinfection and macrolide-resistant M. pneumoniae infection on M. pneumoniae pneumonia in hospitalized children and build a model to predict a severe disease course. Methods: Nasopharyngeal swabs or sputum specimens were collected from patients with community-acquired pneumonia meeting our protocol who were admitted to Shanghai Children’s Medical Center from December 1, 2016, to May 31, 2019. The specimens were tested with the FilmArray Respiratory Panel, a multiplex polymerase chain reaction assay that detects 16 viruses, Bordetella pertussis, M. pneumoniae, and Chlamydophila pneumoniae. Univariate and multivariate logistic regression models were used to identify the risk factors for adenovirus coinfection and macrolide-resistant mycoplasma infection.Results: Among the 107 M. pneumoniae pneumonia patients, the coinfection rate was 56.07%, and 60 (60/107, 56.07%) patients were infected by drug-resistant M. pneumoniae. Adenovirus was the most prevalent coinfecting organism, accounting for 22.43% (24/107). The classification tree confirmed that viral coinfection was more common in patients younger than 3 years old. Adenovirus coinfection and drug-resistant M. pneumoniae infection occurred more commonly in patients with refractory M. pneumoniae pneumonia (P=0.019; P=0.001). A prediction model including wheezing, lung consolidation and extrapulmonary complications was used to predict adenovirus coinfection. The area under the receiver operating characteristic curve of the prediction model was 0.795 (95% CI 0.679-0.893, P<0.001). A prolonged fever duration after the application of macrolides for 48 hours was found more commonly in patients infected by drug-resistant M. pneumoniae (P=0.002). A fever duration longer than 7 days was an independent risk factor for drug-resistant Mycoplasma infection (OR=3.500, 95% CI=1.310-9.353, P=0.012).Conclusions: The occurrence of refractory M. pneumoniae pneumonia is associated with adenovirus coinfection and infection by drug-resistant M. pneumoniae. A prediction model combining wheezing, extrapulmonary complications and lung consolidation can be used to predict adenovirus coinfection in children with M. pneumoniae pneumonia. A prolonged fever duration indicates drug-resistant M. pneumoniae infection, and a reasonable change in antibiotics is necessary.

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iTRAQ-based Quantitative Proteomics Study in Patients with Refractory <i>Mycoplasma pneumoniae</i> Pneumonia

Cited by 19 publications

References 35 publications

Unresolved atelectasis in refractory Mycoplasma pneumoniae pneumonia: Predictive factors and the influence of corticosteroids and bronchoscopy lavage therapy

Unresolved atelectasis in refractory Mycoplasma pneumoniae pneumonia: Predictive factors and the influence of corticosteroids and bronchoscopy lavage therapy

Analysis of the plasma proteome using iTRAQ and TMT‐based Isobaric labeling

Impact of viral coinfection and macrolide-resistant mycoplasma infection in children with refractory Mycoplasma pneumoniae pneumonia

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