iTRAQ-based quantitative proteomics of stratum corneum of dandruff scalp reveals new insights into its aetiology and similarities with atopic dermatitis

Cavusoglu, Nükhet; Delattre, Caroline; Donovan, Mark; Bourassa, Sylvie; Droit, Arnaud; Rawadi, Charles El; Jourdain, Roland; Bernard, Dominique

doi:10.1007/s00403-016-1681-4

Cited by 12 publications

(13 citation statements)

References 61 publications

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“…Similar findings showing increased expression of KLK6 proteases have been reported. 11 The increased expression of KLK5 and KLK6 in the SC of dandruff-affected subjects agreed with reports from previous studies on atopic dermatitis. 16 In a murine atopic dermatitis model, alteration of pH induced upregulated expression of KLK5, leading to skin barrier dysfunction.…”

Section: F I G U R Esupporting

confidence: 91%

Biophysical characteristics of dandruff‐affected scalp categorized on the basis of sebum levels

Yoon¹,

Shim²,

Lim³

et al. 2020

J of Cosmetic Dermatology

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Background: Various sebum levels can be detected in dandruff-affected scalps. However, few studies have compared the biophysical characteristics of dandruff scalps categorized based on sebum levels. Aims: To investigate and compare the biophysical characteristics of dandruff-affected scalps categorized based on sebum levels. Methods: Fifty-four Korean women with dandruff and 30 healthy Korean women underwent physiological measurements, including evaluation of sebum and hydration levels, pH, and transepidermal water loss (TEWL) in the scalp. The levels of the biomarkers of interleukin-8 (IL-8) and kallikrein 5 (KLK5) and corneodesmosomes were investigated in the stratum corneum (SC) of the scalp. Results: Dandruff was categorized as dry (low-sebum, n = 25) or oily (high-sebum, n = 29) based on a sebum cutoff level of 97.82 μg/cm 2. Both dry and oily dandruffaffected scalps showed significantly decreased hydration levels and increased pH and TEWL compared with healthy subjects, with hydration levels being lower in dry dandruff-affected scalps. IL-8 expression was significantly increased in the oily dandruff-affected scalp. In addition, both dry and oily dandruff-affected scalps showed significantly increased KLK5 levels in the SC, with the levels being higher in oily dandruff-affected scalps. Altered distribution of corneodesmosomes, present on the entire surface area of the corneocytes, was notable in oily dandruff-affected scalps. Conclusion: The biophysical characteristics of the two types of dandruff represent the influence of different characteristics, including hydration levels, expression of IL-8 and KLK5, and corneodesmosome distribution. Thus, strategies to reduce dandruff levels should differ according to sebum levels.

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Section: F I G U R Esupporting

confidence: 91%

Biophysical characteristics of dandruff‐affected scalp categorized on the basis of sebum levels

Yoon¹,

Shim²,

Lim³

et al. 2020

J of Cosmetic Dermatology

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“…However, even though innate immune receptors such as TLR2 have been reported to mediate Malassezia sp. cytotoxicity to keratinocytes in vitro , its detailed mechanism remains to be identified in in vivo (Donnarumma et al, 2014 ; Cavusoglu et al, 2016 ). A high abundance of pathways related to general functions and genetic information and processing (sulfur relay system, proteasome pathway, cell cycle in yeast, and meiosis) was also observed in the dandruff scalp compared to the healthy scalp, which showed a significant positive correlation with M. restricta .…”

Section: Discussionmentioning

confidence: 99%

Comparison of Healthy and Dandruff Scalp Microbiome Reveals the Role of Commensals in Scalp Health

Saxena

Mittal

Clavaud

et al. 2018

Front. Cell. Infect. Microbiol.

117

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Several scalp microbiome studies from different populations have revealed the association of dandruff with bacterial and fungal dysbiosis. However, the functional role of scalp microbiota in scalp disorders and health remains scarcely explored. Here, we examined the bacterial and fungal diversity of the scalp microbiome and their potential functional role in the healthy and dandruff scalp of 140 Indian women. Propionibacterium acnes and Staphylococcus epidermidis emerged as the core bacterial species, where the former was associated with a healthy scalp and the latter with dandruff scalp. Along with the commonly occurring Malassezia species (M. restricta and M. globosa) on the scalp, a strikingly high association of dandruff with yet uncharacterized Malassezia species was observed in the core mycobiome. Functional analysis showed that the fungal microbiome was enriched in pathways majorly implicated in cell-host adhesion in the dandruff scalp, while the bacterial microbiome showed a conspicuous enrichment of pathways related to the synthesis and metabolism of amino acids, biotin, and other B-vitamins, which are reported as essential nutrients for hair growth. A systematic measurement of scalp clinical and physiological parameters was also carried out, which showed significant correlations with the microbiome and their associated functional pathways. The results point toward a new potential role of bacterial commensals in maintaining the scalp nutrient homoeostasis and highlights an important and yet unknown role of the scalp microbiome, similar to the gut microbiome. This study, therefore, provides new perspectives on the better understanding of the pathophysiology of dandruff.

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“…Several studies have shown that SASPase has a key role in SC barrier function. Recent proteomics studies of subjects with dandruff scalp showed that there was a significant increase of SASPase in the SC in this condition [9]. Furthermore, de novo missense variants in SASPase in dogs result in aberrant filaggrin expression and an associated ichthyosis skin phenotype [10].…”

Section: Introductionmentioning

confidence: 99%

“…Filaggrin (FLG) mutations are recognized as a major risk factor for AD [20] and mutations in the gene also underlie Ichthyosis vulgaris syndrome [21]. The protein levels of FLG2 are also decreased in the SC and epidermis of AD patients [22,23], in the SC from dandruff condition [9], in a lipid raft disruption model [24] and in ichthyosis peeling skin [19,25] suggesting that FLG2 plays an important role in epidermal homeostasis and barrier formation. In a reconstructed epidermal model where the expression of FLG2 was downregulated by lentivirus mediated shRNA interference, the levels of filaggrin processing enzymes were reduced, resulting in decreased levels of filaggrin derived free amino acids.…”

Section: Introductionmentioning

confidence: 99%

Filaggrin and filaggrin 2 processing are linked together through skin aspartic acid protease activation

et al. 2020

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Skin aspartic acid protease (SASPase) is believed to be a key enzyme involved in filaggrin processing during epidermal terminal differentiation. Since little is known about the regulation of SASPase function, the aim of this study was to identify involved protein partners in the process. Yeast two hybrid analyses using SASPase as bait against a human reconstructed skin library identified that the N-terminal domain of filaggrin 2 binds to the N-terminal fragment of SASPase. This interaction was confirmed in reciprocal yeast two hybrid screens and by Surface Plasmon Resonance analyses. Immunohistochemical studies in human skin, using specific antibodies to SASPase and the N-terminal domain of filaggrin 2, showed that the two proteins partially co-localized to the stratum granulosum. In vitro enzymatic assays showed that the N-terminal domain of filaggrin 2 enhanced the autoactivation of SASPase to its 14 kDa active form. Taken together, the data suggest that the N-terminal domain of filaggrin 2 regulates the activation of SASPase that may be a key event upstream of filaggrin processing to natural moisturizing factors in the human epidermis.

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iTRAQ-based quantitative proteomics of stratum corneum of dandruff scalp reveals new insights into its aetiology and similarities with atopic dermatitis

Cited by 12 publications

References 61 publications

Biophysical characteristics of dandruff‐affected scalp categorized on the basis of sebum levels

Biophysical characteristics of dandruff‐affected scalp categorized on the basis of sebum levels

Comparison of Healthy and Dandruff Scalp Microbiome Reveals the Role of Commensals in Scalp Health

Filaggrin and filaggrin 2 processing are linked together through skin aspartic acid protease activation

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