iTRAQ-based quantitative proteomics and target-fishing strategies reveal molecular signatures on vasodilation of Compound Danshen Dripping Pills

Wu, Xin; Han, Xiujiang; Li, Lili; Fan, Simiao; Zhuang, Pengwei; Zhang, Yanjun

doi:10.1016/j.cbi.2019.108923

Cited by 13 publications

(14 citation statements)

References 24 publications

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“…Mediators of Inflammation pathway, and the AMPK signaling pathway all contribute significantly to the risk of coronary heart disease in postmenopausal women. The cAMP [36] and cGMP-PKG [37] signaling pathways have been implicated in the pathophysiology of coronary atherosclerosis. In the cardiovascular system, an activated AMPK signaling pathway had a protective effect [38].…”

Section: Discussionmentioning

confidence: 99%

Identification of an Epigenetic Signature for Coronary Heart Disease in Postmenopausal Women’s PBMC DNA

Zhong

Song

Gao

et al. 2022

Mediators of Inflammation

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Menopause is accompanied with an increased risk of cardiovascular disease. DNA methylation may have a significant impact on postmenopausal women’s development of coronary heart disease. DNA methylation alterations in peripheral blood mononuclear cells (PBMCs) from women with coronary heart disease and healthy controls were detected using the Illumina Infinium MethylationEPIC BeadChip platform in this work. We employed Sangerbox technology and the GO and KEGG databases to further study the pathogenesis of coronary heart disease in postmenopausal women. After that, we used functional epigenetic module analysis and Cytoscape to remove the hub genes from the protein–protein interaction networks. Five genes (FOXA2, PTRD, CREB1, CTNAP2, and FBN2) were the hub genes. Lipid accumulation, endothelial cell failure, inflammatory responses, monocyte recruitment and aggregation, and other critical biological processes were all influenced by these genes. Finally, we employed methylation-specific PCR to demonstrate that FOXA2 was methylated at a high level in postmenopausal women with coronary heart disease. To better understand coronary heart disease in postmenopausal women’s molecular mechanisms, our study examine the major factors contributing to the state of DNA methylation modification, which will help discover novel diagnostic tools and treatment options.

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Section: Discussionmentioning

confidence: 99%

Identification of an Epigenetic Signature for Coronary Heart Disease in Postmenopausal Women’s PBMC DNA

Zhong

Song

Gao

et al. 2022

Mediators of Inflammation

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“…Therefore, we evaluated the anti-osteoporotic effect of BGSSD and explored the specific cellular phenotype (17). Then, a pharmacodynamic network was conducted to analyze the result of quantitative proteomics based on stable isotope dimethyl labeling (18)(19)(20). Finally, molecular biology was employed to verify the proposed mechanism of alleviating osteoporosis, relying on the PI3K-AKT pathway.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Proteomics Revealed the Pharmacodynamic Network of Bugu Shengsui Decoction Promoting Osteoblast Proliferation

et al. 2022

Front. Endocrinol.

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Background and ObjectiveWith high morbidity and disability, osteoporosis is a worldwide bone metabolism disease, regulated by complex pathological processes. Insufficient osteogenesis is greatly essential to osteoporosis. Traditional Chinese Medicine, a complex natural herbal medicine system, has increasingly attracted attention all over the world. Bugu Shengsui Decoction, a compound formula for osteoporosis, has significant clinical effects in the treatment of osteoporosis. Yet the detailed mechanisms are unclear. Thus, we investigated the effects and mechanism of Bugu Shengsui Decoction on osteoporotic rats and osteoblasts in vitro.MethodsIn this study, we evaluated the effect of Bugu Shengsui Decoction in an animal model of orchiectomy. Multi-pharmacology indexes revealed that Bugu Shengsui Decoction obviously improved bone metabolism, bone mineral density, bone morphology, and biomechanics in the castrated rats. Then, serum pharmacology was employed to unveil that Bugu Shengsui Decoction promoted the proliferation and differentiation of osteoblasts. Moreover, quantitative proteomics combined with RNA interference assay was used to analyze and verify the pathway and key targets in pro-proliferation of MC3T3-E1 cells.ResultsBugu Shengsui Decoction obviously improved the worse parameters of bone metabolism, bone mineral density, bone morphology, and biomechanics in a castrated rat model. In vitro, Bugu Shengsui Decoction exerted proliferation- and differentiation-promoting effects of osteoblasts induced by serum starvation. Moreover, quantitative proteomics analysis combined with RNA interfere assay illustrated that Bugu Shengsui Decoction promoted osteogenesis via the PI3K-AKT pathway.ConclusionSummarily, our discoveries certify that Bugu Shengsui Decoction is an effective treatment for osteoporosis via PI3K-AKT. This study is not only a beneficial attempt to explore the detailed mechanism of Traditional Chinese formula but also will provide inspiration for the treatment strategy of osteoporosis.

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“…Proteomics comprises the large-scale, systematic study of protein structure and function, usually with respect to a defined entity: a pathway, organelle, cell, tissue, or organism. Clinical proteomics, in which mass spectrometry (MS)-based proteomic approaches are used, involve the detailing and quantification of proteomes of normal or diseased tissues to identify peptides, proteins, and post-translational modifications that support early disease detection, facilitate diagnosis, inform prognosis, guide therapy, monitor disease activity, or identify new therapeutic targets [11]. Proteomics represents the current frontier technology and, of late, has been used to study vascular diseases [11,12].…”

Section: Introductionmentioning

confidence: 99%

Proteomic Profiling and Pathway Analysis of Acid Stress-Induced Vasorelaxation of Mesenteric Arteries In Vitro

Mohanty

Banerjee

Mahanty

et al. 2022

Genes

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Although metabolic acidosis is associated with numerous pathophysiological conditions and its vasorelaxation effects have been well described in different animal and culture models, the molecular mechanisms of acidosis-induced vasorelaxation are not fully understood. Mesenteric artery models have been used extensively to examine the vascular response to various pathophysiological conditions. Our previous studies and several other reports have suggested the vascular responses of goat mesenteric arteries and human arteries to various stimuli, including acidic stress, are highly similar. In this study, to further identify the signaling molecules responsible for altered vasoreactivity in response to acidic pH, we examined the proteomic profile of acid stress-induced vasorelaxation using a goat mesenteric artery model. The vascular proteomes under acidic pH were compared using 2D-GE with 7 cm IPG strips and mini gels, LC-MS/MS, and MALDI TOF MS. The unique proteins identified by mass spectroscopy were actin, transgelin, WD repeat-containing protein 1, desmin, tropomyosin, ATP synthase β, Hsp27, aldehyde dehydrogenase, pyruvate kinase, and vitamin K epoxide reductase complex subunit 1-like protein. Out of five protein spots identified as actin, three were upregulated > 2-fold. ATP synthase β was also upregulated (2.14-fold) under acid stress. Other actin-associated proteins upregulated were transgelin, desmin, and WD repeat-containing protein 1. Isometric contraction studies revealed that both receptor-mediated (histamine) and non-receptor-mediated (KCl) vasocontraction were attenuated, whereas acetylcholine-induced vasorelaxation was augmented under acidosis. Overall, the altered vasoreactivity under acidosis observed in the functional studies could possibly be attributed to the increase in expression of actin and ATP synthase β.

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iTRAQ-based quantitative proteomics and target-fishing strategies reveal molecular signatures on vasodilation of Compound Danshen Dripping Pills

Cited by 13 publications

References 24 publications

Identification of an Epigenetic Signature for Coronary Heart Disease in Postmenopausal Women’s PBMC DNA

Identification of an Epigenetic Signature for Coronary Heart Disease in Postmenopausal Women’s PBMC DNA

Quantitative Proteomics Revealed the Pharmacodynamic Network of Bugu Shengsui Decoction Promoting Osteoblast Proliferation

Proteomic Profiling and Pathway Analysis of Acid Stress-Induced Vasorelaxation of Mesenteric Arteries In Vitro

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