iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings

Abstract

Background As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Methods To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. Result In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper cultivars in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher ROS scavenging, photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180.

Responses of the tree peony (Paeonia suffruticosa, Paeoniaceae) cultivar ‘Yu Hong’ to heat stress revealed by iTRAQ-based quantitative proteomics

“…Plants have evolved efficient enzymatic antioxidant systems to deal with the peroxidative damage of ROS during their long-term adaptive evolution [ 61 ]. POD (c100066.graph_c1), APX (c115075.graph_c0), GPX (c114484.graph_c0), and CAT (c116374.graph_c0), all of which are enzymatic antioxidants of the enzymatic antioxidant system, were among the up-regulated antioxidant enzymes in this study [ 23 , 37 , 38 ]. In response to heat stress, up-regulated production of these proteins is necessary for scavenging ROS.…”

“…Respiration, the main metabolic pathway metabolism of carbohydrates, is an enzymatic oxidation reaction and is an important pathway for energy conversion in plants [ 37 ]. In this study, 1 citrate synthase (c104351.graph_c0), 1 fructose- 1,6- bisphosphate aldolase (c41383.graph_c0), 1 phosphoglycerate kinase (c117676.graph_c3) and 1 Malate dehydrogenase (c112840.graph_c0) were up-regulated, and both of them are important regulatory enzymes in glycolysis pathway and tricarboxylic acid cycle pathway in carbohydrate metabolism [ 73 ].…”

“…Stress perception, signal transduction, and the expression of certain stress-related genes and metabolites all play a role in plant adaptation to environmental stress [ 36 ]. Signal transduction is a complex regulatory system in plants that plays an important role in sustaining their normal growth and development in response to abiotic stresses [ 37 , 38 ]. The importance of Ca 2+ as a second messenger in the signal transduction pathway is well understood [ 39 ].…”

“…Abiotic stress causes a transitory rise in cytoplasmic Ca 2+ , either through exosomal inward flow or the release of internal reserves [ 40 ]. Calmodulin is the most well-known Ca 2+ sensor in signal transduction, and it is involved in heat shock signaling [ 37 ]. Calcium-dependent protein kinase is a serine/threonine protein kinase with c-terminal calmodulin-like structural domain at its c-terminus, making it both a Ca 2+ sensor and an effector of Ca 2+ signal transduction [ 39 , 40 ].…”

Transcriptomics Analysis of Heat Stress-Induced Genes in Pepper (Capsicum annuum L.) Seedlings

“…Pepper (Capsicum annuum L.) is an important member of the Solanaceae family and is one of the most important spice and vegetable crops in many countries [1]. It is rich in capsaicin, capsanthin, and vitamins, which can improve appetite and health [2]. Pepper grows well in warm climates but is sensitive to high temperatures, with the suitable temperature range for growth and development being 20-30 • C [3].…”