iTRAQ-based quantitative proteomic analysis of Pseudomonas aeruginosa SJTD-1: A global response to n-octadecane induced stress

Liu, Huan; Sun, Wen‐Hua; Liang, Rubing; Huang, Li; Hou, Jingli; Liu, Jianhua

doi:10.1016/j.jprot.2015.03.034

Cited by 39 publications

(24 citation statements)

References 66 publications

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“…In our previous research, we also proposed that the FadL and FadD would play an important role in uptake alkanes in P . aeruginosa [22]. In this comparative proteomics analysis, FadL has an abundance expression by both label free and iTRAQ-based methods comparing cells cultivated on C18 with C16, and this result has been further confirmed by qRT-PCR.…”

Section: Discussionmentioning

confidence: 64%

“…“Bacterial chemotaxis” was found to be the important pathway, of which the proteins included showed the strong interaction among them by protein-protein interaction network analysis. Chemotaxis pathways have been found to mediate flagellum- and type IV-pili mediated motility, playing important role in access compounds that serve as carbon/nitrogen sources or electron acceptors [22,32]. In sum, the results of comparative proteomics suggested chemotaxis still play an important role in responds to n-octadecane comparing with n-hexadecane in order to acquire alkanes of longer carbon chain.…”

Section: Discussionmentioning

confidence: 99%

“…The data analysis method of iTRAQ-based proteomic analysis was the same as the mentioned before [22]. The database being used to search is the NCBI_SJTD1_20140411 database (5618 entries) which was established as the mentioned before [22].…”

Section: Methodsmentioning

confidence: 99%

“…The database being used to search is the NCBI_SJTD1_20140411 database (5618 entries) which was established as the mentioned before [22]. Scaffold Q+ (version_4.4.0, Proteome Software Inc., Portland, OR) was used to quantify the isobaric tag peptide and protein identities.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, we use iTRAQ strategy firstly to compare the proteomes of P . aeruginosa SJTD-1 degrading alkane [22]. Our previous results indicate FadL and type VI secretion system (T6SS) subunits might play significant roles in alkane uptake in P .…”

Section: Introductionmentioning

confidence: 99%

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Quantitative proteomics analysis of proteins involved in alkane uptake comparing the profiling of Pseudomonas aeruginosa SJTD-1 in response to n-octadecane and n-hexadecane

et al. 2017

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While many data are available on genes encoding proteins for degradation of hydrocarbons in bacteria, the impact of alkane on transporter protein expression is unclear. Pseudomonas aeruginosa SJTD-1 is a strain that can consume medium- and long-chain n-alkanes. In order to study the proteins involved in n-octadecane uptake, we use iTRAQ and label free comparative proteomics analysis to identify the proteins of alkane uptake in response to n-octadecane (C18) comparing with n-hexadecane (C16) in P. aeruginosa SJTD-1. A total of 1102 and 1249 proteins were identified by iTRAQ-based and label free quantitative methodologies, respectively. By application of 1.5 (iTRAQ) or 2-fold (label free) for upregulated and 0.65 (iTRAQ) or 0.5-fold (label free) for downregulated cutoff values, 91 and 99 proteins were found to be differentially expressed comparing SJTD-1 cultivated on C18 with C16 respectively. There are six proteins with the common differential expression by iTRAQ and label free-based methods. Results of bioinformational analysis suggested the involvement of bacterial chemotaxis in responds to C18. Additionally, quantitative reverse transcriptase PCR (qRT-PCR) results confirmed C18-induced change in levels of FleQ, FliC, NirS, FadL and FadD proteins and the role of the proteins in n-octadecane uptake was further discussed in P. aeruginosa. In conclusion, results of the present study provided information about possible target-related proteins of bacterial chemotaxis, swimming performance, alkane transport to stimulus of n-ctadecane rather than n-hexadecane in P. aeruginosa SJTD-1.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%