iTRAQ‐Based Quantitative Proteomic Analysis of Nasopharyngeal Carcinoma

Cai, Xinzhang; Zeng, Weiqun; Xiang, Yong‐Bing; Liu, Yi; Zhang, Hongmin; Li, Hong; She, Sha; Yang, Min; Xia, Kun; Peng, Shifang

doi:10.1002/jcb.25105

Cited by 10 publications

(9 citation statements)

References 60 publications

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“…Nucleolin has been identified to be markedly upregulated in NPC tissues by iTRAQ and tissue microarray methods and has been confirmed to be expressed on the surfaces of malignant cell-derived EVs 62 . However, the value of EV-derived nucleolin has not been explored in NPC.…”

Section: Resultsmentioning

confidence: 99%

An ultrasensitive hybridization chain reaction-amplified CRISPR-Cas12a aptasensor for extracellular vesicle surface protein quantification

Xing¹,

Lù²,

Huang³

et al. 2020

Theranostics

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Tumor-derived extracellular vesicle (TEV) protein biomarkers facilitate cancer diagnosis and prognostic evaluations. However, the lack of reliable and convenient quantitative methods for evaluating TEV proteins prevents their clinical application. Methods: Here, based on dual amplification of hybridization chain reaction (HCR) and CRISPR-Cas12a, we developed the apta-HCR-CRISPR assay for direct high-sensitivity detection of TEV proteins. The TEV protein-targeted aptamer was amplified by HCR to produce a long-repeated sequence comprising multiple CRISPR RNA (crRNA) targetable barcodes, and the signals were further amplified by CRISPR-Cas12a collateral cleavage activities, resulting in a fluorescence signal. Results: The established strategy was verified by detecting the TEV protein markers nucleolin and programmed death ligand 1 (PD-L1). Both achieved limit of detection (LOD) values as low as 10 2 particles/µL, which is at least 10 4 -fold more sensitive than aptamer-ELISA and 10 2 -fold more sensitive than apta-HCR-ELISA. We directly applied our assay to a clinical analysis of circulating TEVs from 50 µL of serum, revealing potential applications of nucleolin + TEVs for nasopharyngeal carcinoma cancer (NPC) diagnosis and PD-L1 + TEVs for therapeutic monitoring. Conclusion: The platform was simple and easy to operate, and this approach should be useful for the highly sensitive and versatile quantification of TEV proteins in clinical samples.

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Section: Resultsmentioning

confidence: 99%

An ultrasensitive hybridization chain reaction-amplified CRISPR-Cas12a aptasensor for extracellular vesicle surface protein quantification

Xing¹,

Lù²,

Huang³

et al. 2020

Theranostics

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“…108 113 Additionally, nasopharyngeal carcinomas have low expression of SCCA1. 114 In contrast to the tissue proteomic levels, the pretreated OSCC patients have elevated serum level of SCCA1, correlating with disease progression. 108,115,116 The enhancement in the serum SCCA1 levels has been commonly reported in patients suffering from squamous cell carcinomas of head and neck, oesophagus, lung, vulva, and cervix.…”

Section: Discussionmentioning

confidence: 97%

Assessment of cellular and serum proteome from tongue squamous cell carcinoma patient lacking addictive proclivities for tobacco, betel nut, and alcohol: Case study

Khowal

Naqvi

Monga

et al. 2018

J of Cellular Biochemistry

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The intriguing molecular pathways involved in oral carcinogenesis are still ambiguous. The oral squamous cell carcinoma (OSCC) ranks as the most common type constituting more than 90% of the globally diagnosed oral cancers cases. The elevation in the OSCC incidence rate during past 10 years has an alarming impression on human healthcare. The major challenges associated with OSCC include delayed diagnosis, high metastatic rates, and low 5-year survival rates. The present work foundations on reverse genetic strategy and involves the identification of genes showing expressional variability in an OSCC case lacking addictive proclivities for tobacco, betel nut, and/or alcohol, major etiologies. The expression modulations in the identified genes were analyzed in 16 patients comprising oral pre-cancer and cancer histo-pathologies. The genes SCCA1 and KRT1 were found to down regulate while DNAJC13, GIPC2, MRPL17, IG-Vreg, SSFA2, and UPF0415 upregulated in the oral pre-cancer and cancer pathologies, implicating the genes as crucial players in oral carcinogenesis.

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“…However, the molecular mechanisms for the tumor suppressive functions of FH are uncharacterized. HSPB1 has been reported as a multifunctional molecule; for example, the expression level of HSPB1 is higher in nasopharyngeal carcinoma compared with the adjacent non-tumor tissues (51). HSPB1 has also been demonstrated to suppress pulmonary fibrosis and lung tumorigenesis through inhibiting the EndMT.…”

Section: Discussionmentioning

confidence: 99%

Quantitative proteomic analysis of the miR‑148a‑associated mechanisms of metastasis in non‑small cell lung cancer

Chu

Lin

et al. 2018

Oncol Lett

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MicroRNAs (miRs) are small non-coding RNAs that regulate gene expression and protein synthesis. Our previous study demonstrated that miR-148a suppressed the metastasis of non-small cell lung cancer (NSCLC) and. However, the modulatory mechanism of this effect remains unclear. In the present study, quantitative proteomic technology was used to study the protein expression profile of SPC-A-1 cells subsequent to the downregulation of miR-148a expression, in order to elucidate the molecular mechanism of the suppression of NSCLC metastasis by miR-148a. The differentially expressed proteins identified were analyzed using bioinformatics tools, including the Database for Annotation, Visualization and Integrated Discovery and the Search Tool for the Retrieval of Interacting Genes/proteins. In two experiments, 4,048 and 4,083 proteins were identified, and 4,014 and 4,039 proteins were quantified, respectively. In total, 44 proteins were upregulated and 40 proteins were downregulated. This was verified at the protein and mRNA levels by western blotting and reverse transcription-quantitative polymerase chain reaction, respectively. Bioinformatics analysis was used to identify potential interactions and signaling networks for the differentially expressed proteins. This may have provided an appropriate perspective for the comprehensive analysis of the modulatory mechanism underlying the metastasis-suppressive effects of miR-148a in NSCLC. In conclusion, quantitative proteomic technology revealed that miR-148a may regulate a panel of tumor-associated proteins to suppress metastasis in NSCLC.

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iTRAQ‐Based Quantitative Proteomic Analysis of Nasopharyngeal Carcinoma

Cited by 10 publications

References 60 publications

An ultrasensitive hybridization chain reaction-amplified CRISPR-Cas12a aptasensor for extracellular vesicle surface protein quantification

An ultrasensitive hybridization chain reaction-amplified CRISPR-Cas12a aptasensor for extracellular vesicle surface protein quantification

Assessment of cellular and serum proteome from tongue squamous cell carcinoma patient lacking addictive proclivities for tobacco, betel nut, and alcohol: Case study

Quantitative proteomic analysis of the miR‑148a‑associated mechanisms of metastasis in non‑small cell lung cancer

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