iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A

Abstract

a b s t r a c tTetrabromobisphenol A (TBBPA) accounts for the largest production of brominated flame-retardants (BFRs) along the Laizhou Bay in China and is the most widely used BFR in industrial products. It can induce diverse toxicities including hepatotoxicity, nephrotoxicity, neurotoxicity and endocrine disrupting effects in mammalian and fish models. In this work, we applied iTRAQ-based proteomics to investigate the gender-specific responses in mussel Mytilus galloprovincialis to TBBPA. Thirty-one proteins were differentially expressed in hepatopancreas between male and female mussels, which clearly indicated the biological differences between male and female mussels at the protein level. After exposure of TBBPA (18.4 nmol/L) for one month, a total of 60 proteins were differentially expressed in response to the TBBPA treatment in mussel hepatopancreas, among which 33 and 29 proteins were significantly altered in TBBPA-treated male and female mussel samples, respectively. Only two of the 60 proteins were commonly altered in both male and female mussel samples exposed to TBBPA. Based on KEGG analysis, these differentially expressed proteins of TBBPA-induced effects were assigned to several groups, including cytoskeleton, reproduction and development, metabolism, signal transduction, gene expression, stress response and apoptosis. Overall, results indicated that TBBPA exposure could induce apoptosis, oxidative and immune stresses and disruption in energy, protein and lipid metabolisms in both male and female mussels with different mechanisms. This work suggested that the gender differences should be considered in ecotoxicoproteomics.

Proteomic and metabolomic responses in D-shape larval mussels Mytilus galloprovincialis exposed to cadmium and arsenic

“…As a flavoprotein, NADH dehydrogenase processes iron-sulfur centers and is involved in production of reactive oxygen species in mitochondria [30]. Peptidylprolyl isomerase A is a known protein recognized as the marker of apoptosis that is also related to oxidative stress [31]. Therefore, the significant up-regulation of manganese superoxide dismutase, NADH dehydrogenase and peptidylprolyl isomerase A indicated the clear oxidative stress and consequent apoptosis induced by Cd in D-shape larval mussels.…”

Proteomic responses reveal the differential effects induced by cadmium in mussels Mytilus galloprovincialis at early life stages

“…As a flavoprotein, NADH dehydrogenase contains iron-sulfur centers and is involved in production of reactive oxygen species in mitochondria [29]. These two proteins, profilin and NADH dehydrogenase, are known to be related to oxidative stress [29,30]. Therefore, the alteration of profilin and NADH dehydrogenase confirmed the oxidative stress induced by Cd in juvenile mussels.…”

“…The downregulation of this protein confirmed the immune stress induced by Cd in juvenile mussels, as marked by the down-regulated apextrin-like protein. Peptidylprolyl isomerase A, sorting nexin 6 and collagen alpha-6(VI) chain isoform X3 are known proteins recognized as the markers of apoptosis [30,34,35]. The alterations of these three proteins suggested that Cd exposure caused apoptosis in juvenile mussels.…”

Comparison of immune responses and antioxidant status of different generations of growth-selectedPortunus trituberculatusfamilies

“…Results indicated that mRNA expression levels of PO and CAT did not correlate with enzyme activities. The disparity between mRNA and corresponding protein expressions was not surprising as mRNA expression may not be consistent with the corresponding protein expression because of post‐transcriptional and post‐translational modifications (Ji, Wu, Wei & Zhao ).…”