iTRAQ‐based quantitative proteome revealed metabolic changes of <scp><i>Sitophilus zeamais</i></scp> in response to terpinen‐4‐ol fumigation

Huang, Yong; Liao, Min; Yang, Qiwen; Xiao, Jinjing; Hu, Zhaoyin; Cao, Haiqun

doi:10.1002/ps.5135

Cited by 18 publications

(12 citation statements)

References 53 publications

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“…Insect P450s were found in all tissues and performed important functions such as the degradation of drugs, pesticides, and plant secondary metabolites. 39,40 Genomes of different insects have revealed dynamic changes in the number of CYP genes, ranging from 37 in the body louse Pediculus humanus to 204 in the mosquito Culex quinquefasciatus. [41][42][43] In this study, 66 P450s with complete ORFs were identified and named from the transcriptome of M. separata integument.…”

Section: Discussionmentioning

confidence: 99%

Expression and functional analysis of cytochrome P450 genes in the integument of the oriental armyworm, Mythimna separata (Walker)

Huang

Yin

Zhu

et al. 2020

Pest Management Science

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BACKGROUND: Mythimna separata is a devastating agricultural pest that has recently developed insecticide resistance. Integument-specific cytochrome P450s were reported to participate in cuticle formation and could be potential targets for pesticide synthesis. RESULTS: The transcriptome of integuments of M. separata larvae was constructed, generating a total of 38 058 unigenes with an average length of 1243 bp. These unigenes are enriched in functional categories such as lipid transport and metabolism, and secondary metabolites biosynthesis, transport and catabolism. Amongst unigenes, cytochrome P450s were identified and 66 unique P450s with complete open reading frames were named. These P450s were divided into 17 families and 32 subfamilies, containing conserved motifs such as helix C, helix I, helix K, and the heme-binding region. RNA-Seq and RT-qPCR analyses showed different expression levels of P450s in integuments of M. separata larvae. Further RT-qPCR analysis of P450s among different tissues showed that five P450s, especially CYP4G199, were specifically highly expressed in integuments. Moreover, knockdown of CYP4G199 disturbed cuticle formation, leading to imperfection in larval cuticle, and prevented pupation of M. separata. CONCLUSION: Transcriptome of larval integuments provided sequence and expression of genes in M. separata. CYP4G199 is specifically highly expressed in larval integuments and is important for cuticle formation in M. separata.

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Section: Discussionmentioning

confidence: 99%

Expression and functional analysis of cytochrome P450 genes in the integument of the oriental armyworm, Mythimna separata (Walker)

Huang

Yin

Zhu

et al. 2020

Pest Management Science

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“…After removal of the puparium, the whole body of the pupa was disrupted in a lysis buffer (8 M urea, 40 mM Tris-HCl with 1 mM phenylmethane sufonyl fluoride, 2 mM EDTA and 10 mM dithiothreitol, pH 8.5) with a protease inhibitor by TissueLyser (Huang et al, 2018). After centrifuging at 25,000 g for 20 min, the supernatant was carefully removed and mixed with 5 vol of cold acetone and stored at −20°C overnight.…”

Section: Methodsmentioning

confidence: 99%

Changes in Energy Metabolism Trigger Pupal Diapause Transition of Bactrocera minax After 20-Hydroxyecdysone Application

et al. 2019

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Correct timing of diapause entry and exit is critical for a species' survival. While many aspects of insect diapause are well-studied, the mechanisms underlying diapause termination remain largely unknown. The Chinese citrus fly, Bactrocera minax, is a univoltine insect with an obligatory pupal diapause. The application of 20-hydroxyecdysone (20E) is known to terminate diapause in B. minax, and we used this approach, along with isobaric tags for relative and absolute quantitation technology, to determine the proteins associated with diapause termination in this fly. Among 2,258 identified proteins, 1,169 proteins significantly differed at 1, 2, and 5 days post-injection of 20E, compared with the solvent-injected control group. Functional annotation revealed that the majority of differentially expressed proteins were enriched in the core energy metabolism of amino acids, proteins, lipids, and carbohydrates as well as in signal transduction pathways including PPAR signaling, Calcium signaling, Glucagon signaling, VEGF signaling, Ras signaling, cGMP-PKG signaling, and cAMP signaling. A combined transcriptomic and proteomic analysis suggested the involvement of energy metabolism in the response of diapause transition. RNA interference experiments disclosed that a 20E injection triggers diapause termination probably through non-genomic actions, rather than nuclear receptor mediated genomic actions. Our results provide extensive proteomic resources for insect diapause transition and offer a potential for pest control by incapacitating the regulation of diapause termination either by breaking diapause prematurely or by delaying diapause termination to render diapausing individuals at a high risk of mortality.

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“…The IPEC-J2 cells were frozen with liquid nitrogen immediately and then lysed in the “lysis buffer (8 M Urea, 40 mM Tris–HCl with 1 mM PMSF, 2 mM EDTA, and 10 mM DTT, pH 8.5)” (Li et al, 2018). “After centrifugation at 25,000 g at 4°C for 20 min, the supernatant obtained was reduced with 10 mM DTT at 56°C for 1 h, and then alkylated with 55 mM iodoacetamide (IAM) in the dark at room temperature for 45 min” (Huang et al, 2018). Following further centrifugation with 25,000 g at 4°C, the supernatant was quantified by Bradford method.…”

Section: Methodsmentioning

confidence: 99%

Lactobacillus gasseri LA39 Activates the Oxidative Phosphorylation Pathway in Porcine Intestinal Epithelial Cells

Zheng

et al. 2018

Front. Microbiol.

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Intestinal microbial interactions with the host epithelium have important roles in host health. Our previous data have suggested that Lactobacillus gasseri LA39 is the predominant intestinal Lactobacillus in weaned piglets. However, the regulatory role of L. gasseri LA39 in the intestinal epithelial protein expression in piglets remains unclear. In the present study, we conducted comparative proteomics approach to investigate the intestinal epithelial protein profile alteration caused by L. gasseri LA39 in piglets. The expressions of 15 proteins significantly increased, whereas the expressions of 13 proteins significantly decreased in the IPEC-J2 cells upon L. gasseri LA39 treatment. Bioinformatics analyses, including COG function annotation, GO annotation, and KEGG pathway analysis for the differentially expressed proteins revealed that the oxidative phosphorylation (OXPHOS) pathway in IPEC-J2 cells was significantly activated by L. gasseri LA39 treatment. Further data indicated that two differentially expressed proteins UQCRC2 and TCIRG1, associated with the OXPHOS pathway, and cellular ATP levels in IPEC-J2 cells were significantly up-regulated by L. gasseri LA39 treatment. Importantly, the in vivo data indicated that oral gavage of L. gasseri LA39 significantly increased the expression of UQCRC2 and TCIRG1 and the cellular ATP levels in the intestinal epithelial cells of weaned piglets. Our results, both in vitro and in vivo, reveal that L. gasseri LA39 activates the OXPHOS pathway and increases the energy production in porcine intestinal epithelial cells. These findings suggest that L. gasseri LA39 may be a potential probiotics candidate for intestinal energy production promotion and confers health-promoting functions in mammals.

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iTRAQ‐based quantitative proteome revealed metabolic changes of Sitophilus zeamais in response to terpinen‐4‐ol fumigation

Cited by 18 publications

References 53 publications

Expression and functional analysis of cytochrome P450 genes in the integument of the oriental armyworm, Mythimna separata (Walker)

Expression and functional analysis of cytochrome P450 genes in the integument of the oriental armyworm, Mythimna separata (Walker)

Changes in Energy Metabolism Trigger Pupal Diapause Transition of Bactrocera minax After 20-Hydroxyecdysone Application

Lactobacillus gasseri LA39 Activates the Oxidative Phosphorylation Pathway in Porcine Intestinal Epithelial Cells

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