iTRAQ-based quantitative analysis of age-specific variations in salivary proteome of caries-susceptible individuals

Wang, Kun; Wang, Xiuqing; Zheng, Sainan; Niu, Yumei; Zheng, Wenyue; Qin, Xi; Li, Zhongcheng; Luo, Jingjie; Jiang, Wentao; Zhou, Xuedong; Li, Wei; Zhang, Linglin

doi:10.1186/s12967-018-1669-2

Cited by 34 publications

(32 citation statements)

References 35 publications

(41 reference statements)

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“…Next-generation sequencing technologies have been successfully applied in oral microbial analysis [ 12 – 14 ]. The isobaric tags for relative and absolute quantitation (iTRAQ) is a new technique which uses isotopes to label polypeptides for comparing proteomes quantitatively [ 15 , 16 ]. To the best of our knowledge, previous studies of caries-related microbiome and proteome were detached.…”

Section: Introductionmentioning

confidence: 99%

The oral microbiome and salivary proteins influence caries in children aged 6 to 8 years

et al. 2020

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Background Oral microbiome and salivary proteins play a critical role in the occurrence and development of caries. In this study, we used metagenomic and metaproteomic analyses to explore the microbiological and proteinic biomarkers and investigate the etiology of caries in 6–8 years old children. Our study aims to offer a better comprehension of these factors and the relationship with caries, and these findings might facilitate caries risk assessment and provide a basis for future prevention strategies. Methods Children 6 to 8 years old living in rural isolated areas including 40 caries-active subjects and 40 caries-free subjects were recruited. Supragingival plaque and unstimulated saliva were collected for 16S rDNA pyrosequencing and isobaric tags for relative and absolute quantitation (iTRAQ) technique coupled with quantitative nano-flow liquid chromatography-tandem mass spectrometry (LC-MS/MS), respectively. Results We found 6 phyla and 13 genera predominant in all the samples, and differences in relative abundances can be observed. The Alpha diversity analysis demonstrated that the richness and diversity of the bacterial communities were similar between children with caries-free and caries-active groups; LEfSe detected differences in the bacterial community including Dialister, Selenomonas, Actinomyces, and Mogibacterium in the caries-active group (P < 0.05) and Capnocytophaga, Fusobacterium, Desulfuromonadales, Haemophilus, and Porphyromonas in the caries-free group(P < 0.05). The core microbiome was defined as 18 predominant genera in children with caries. The results of the salivary proteome identified 9135 unique peptides and 1662 proteins group from 20 salivary samples. Two hundred fifty-eight proteins were differentially expressed between the caries-free and caries-active groups. Conclusions The diversity of the microbial community has little effect on caries but some bacteria with different relative abundance between the caries-active and caries-free group could be considered as potential biomarkers for children with caries. In addition, as a critical host factor of caries, the salivary proteins are different in caries-free and caries-active groups.

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Section: Introductionmentioning

confidence: 99%

The oral microbiome and salivary proteins influence caries in children aged 6 to 8 years

et al. 2020

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“…As concerns the organic components, in addition to the salivary concentration, they also measured the total amount secreted into the oral cavity, concluding that in the elderly the total values of most salivary components decrease parallel to the salivary flow rate 22 . In 2014, Nassar et al found a decrease in salivary calcium level in elderly people compared to young 23 . Conversely, Sevon et al reported elevated levels of salivary calcium in elderly women, with a peak at around the age of 50 years, compared to that of young women.…”

Section: Changes In Salivary Compositionmentioning

confidence: 99%

“…On the other hand, in their 2014 study Nassar et al assessed that the salivary buffering capacity is not related to age. As most of the salivary bicarbonate is secreted by the parotid gland, the parotid gland function seems to be not prejudiced by aging 23 . A lower salivary concentration of basic fibroblast growth factor (bFGF) has been reported in elderly subjects.…”

Section: Changes In Salivary Compositionmentioning

confidence: 99%

The secretory senescence of the senses of smell and taste

Piccininni¹,

Barbara²,

Luca³

et al. 2020

JGG

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“…With the rapid development of proteomics technology and the application of high resolution mass spectrometry, isobaric tags for relative and absolute quantification (iTRAQ) is currently the most widely used technology in quantitative proteomics [6]. The core principle of iTRAQ is polypeptide labeling and quantification, which resolves quantitative changes in protein intensities with high sensitivity, accuracy, and reproducibility [7]; it is widely used in protein biomarker validation to support preclinical and clinical studies [8].In a previous iTRAQ technology study of silicosis, researchers employed SiO 2 to stimulate raw264.7 cells [9], and TGF-β to stimulate lung fibroblasts [10].…”

Section: Introductionmentioning

confidence: 99%

Protein Expression Profile in Rat Silicosis Model Reveals Upregulation of PTPN2 and Its Inhibitory Effect on Epithelial-Mesenchymal Transition by Dephosphorylation of STAT3

Zhu

Yao

Duan

et al. 2020

IJMS

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Silicosis is a chronic occupational lung disease caused by long-term inhalation of crystalline silica particulates. We created a rat model that closely approximates the exposure and development of silicosis in humans. Isobaric tags for relative and absolute quantitation (iTRAQ) technologies we used to identify proteins differentially expressed in activated rat lung tissue. We constructed three lentiviral knockdown vectors and an overexpression vector for the protein tyrosine phosphatase non-receptor type 2 (PTPN2) gene to achieve stable long-term expression. A total of 471 proteins were differentially expressed in the silicosis group compared with controls. Twenty upregulated, and eight downregulated proteins exhibited a ≥1.5-fold change relative to controls. We next found that the PTPN2, Factor B, and VRK1 concentrations in silicotic rats silicosis and SiO2-stimulated MLE-12 cells were significantly higher than control groups. More importantly, we found that overexpression of PTPN2 simultaneously decreased the expression of phospho–signal transducer and activator of transcription 3 (p-STAT3) and Vimentin, while increasing E-cadherin expression. The opposite pattern was observed for PTPN2-gene silencing. We identified three proteins with substantially enhanced expression in silicosis. Our study also showed that PTPN2 can inhibit epithelial-mesenchymal transition by dephosphorylating STAT3 in silicosis fibrosis.

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iTRAQ-based quantitative analysis of age-specific variations in salivary proteome of caries-susceptible individuals

Cited by 34 publications

References 35 publications

The oral microbiome and salivary proteins influence caries in children aged 6 to 8 years

The oral microbiome and salivary proteins influence caries in children aged 6 to 8 years

The secretory senescence of the senses of smell and taste

Protein Expression Profile in Rat Silicosis Model Reveals Upregulation of PTPN2 and Its Inhibitory Effect on Epithelial-Mesenchymal Transition by Dephosphorylation of STAT3

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