iTRAQ-based proteomics screen for potential regulators of wheat (Triticum aestivum L.) root cell wall component response to Al stress

Yang, Ye; Ma, Li; Zeng, Hong; Chen, Luyao; Zheng, Yun; Li, Caixia; Yang, Zeping; Wu, Nan; Mu, Xi; Dai, Chuan‐Chao; Guan, Huilin; Cui, Xiuming; Liu, Ying

doi:10.1016/j.gene.2018.07.008

Cited by 12 publications

(5 citation statements)

References 66 publications

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“…Lignin deposition and ligni cation in plant cell wall can decrease cell wall plasticity and restrict cell complication(Rui et al 2016). Some researchers reported that the inhibition of root growth in response to Cd, Cu or Al stress was obviously consistent with the increase of root cell ligni cation in several plant species(Christophe et al 2017;Liu et al 2015;Yang et al 2018). It was worth noting that the reduction of root growth was mainly caused by an inhibition of cell elongation rather than cell division when plants were exposed to heavy metal stress(Kollmeier et al 2000) Yang et al (2018).…”

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confidence: 99%

WITHDRAWN: Lignin metabolism involved in zinc-alleviating effects on iron-induced phytotoxicity in wheat roots

Zhang

et al. 2022

Preprint

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In order to reveal the mechanisms of Fe-inhibited plant root growth and Zn-alleviating effects on iron (Fe) toxicity, wheat cultivars Xihan 4 seedlings were used to investigate the relationship between root growth and synthetic metabolism of lignin in response to 300 µM Fe stress alone or in combination with 50 or 250 µM zinc (Zn). Fe-alone-exposed roots exhibited significant increase of cell wall lignin content and the stimulation of phenylalanine ammonia-lyase (PAL), 4-coumarate:CoA ligase (4CL), cinnamyl alcohol dehydrogenase (CAD), laccase (LAC), cell wall bound guaiacol peroxidase activity (CW-bound GPX) and conifers alcohol peroxidase (CAPX) activities as well as the up-regulation of TaPAL, Ta4CL, TaCAD and TaLAC expression. In comparison with Fe stress alone, the presence of Zn effectively alleviated the changes of these parameters in Fe-treated roots. And also, the results of lignin staining in the root tissue were consistent with those of lignin detection in wheat seedlings under different treatments. Further study showed the increase of apoplastic hydrogen peroxide (H2O2) content in conjunction with the stimulation of CW-bound nicotinamide adenine dinucleotide (NADH) oxidase, plasma membrane (PM) nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and apoplastic superoxide dismutase (SOD) in Fe-alone-stressed roots compared with untreated ones, but Fe + Zn treatment partially reversed the changes of these parameters in comparison with Fe exposure alone. Furthermore, the application of exogenous H2O2 not only enhanced the amount of cell wall lignin in Zn + Fe-treated roots but also significantly blocked Zn-alleviating effect on Fe-induced inhibition of root growth. In conclusion, the inhibition of root growth was associated with the increased accumulation of cell wall lignin in response to Fe exposure alone, which was due to the stimulation of lignin metabolizing enzymes and the up-regulation of related enzyme gene expression and the increase of apoplastic H2O2 generation in wheat roots under Fe exposure alone. It was also suggested that Zn-alleviating effect on Fe-induced inhibition of root growth might be related to the reduction of lignin content of root cell wall resulting from the decrease of apoplastic H2O2 content when wheat seedlings were exposed to Fe + Zn treatment.

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confidence: 99%

WITHDRAWN: Lignin metabolism involved in zinc-alleviating effects on iron-induced phytotoxicity in wheat roots

Zhang

et al. 2022

Preprint

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“…Function correlation showed DEGs and DAPs, such as kinesin-like protein, (1->3)-beta-glucan endohydrolase, expansin-like protein, extensin, and dynamin GTPase related to cell wall biosynthesis and modifications, to be higher in the tolerant genotypes under Al stress conditions when compared with the sensitive one. A similar approach to detect potential regulator related to wheat root cell wall under Al stress conditions was reported by Yang et al (2018).…”

Section: Discussionmentioning

confidence: 95%

Comparative Inter- and IntraSpecies Transcriptomics Revealed Key Differential Pathways Associated With Aluminium Stress Tolerance in Lentil

Singh

Chaudhary

et al. 2021

Front. Plant Sci.

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Aluminium stress causes plant growth retardation and engenders productivity loss under acidic soil conditions. This study accentuates morpho-physiological and molecular bases of aluminium (Al) tolerance within and between wild (ILWL-15) and cultivated (L-4602 and BM-4) lentil species. Morpho-physiological studies revealed better cyto-morphology of tolerant genotypes over sensitive under Al3+ stress conditions. Mitotic lesions were observed in root cells under these conditions. Transcriptome analysis under Al3+ stress revealed 30,158 specifically up-regulated genes in different comparison groups showing contigs between 15,305 and 18,861 bp. In tolerant genotypes, top up-regulated differentially expressed genes (DEGs) were found to be involved in organic acid synthesis and exudation, production of antioxidants, callose synthesis, protein degradation, and phytohormone- and calcium-mediated signalling under stress conditions. DEGs associated with epigenetic regulation and Al3+ sequestration inside vacuole were specifically upregulated in wild and cultivars, respectively. Based on assembled unigenes, an average of 6,645.7 simple sequence repeats (SSRs) and 14,953.7 high-quality single nucleotide polymorphisms (SNPs) were spotted. By quantitative real-time polymerase chain reaction (qRT-PCR), 12 selected genes were validated. Gene ontology (GO) annotation revealed a total of 8,757 GO terms in three categories, viz., molecular, biological, and cellular processes. Kyoto Encyclopaedia of Genes and Genomes pathway scanning also revealed another probable pathway pertaining to metacaspase-1,−4, and −9 for programmed cell death under Al-stress conditions. This investigation reveals key inter- and intraspecies metabolic pathways associated with Al-stress tolerance in lentil species that can be utilised in designing future breeding programmes to improve lentil and related species towards Al3+ stress.

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“…Kyoto Encyclopedia of Genes and Genomes (KEGG) 3 was used to analyze the major metabolic pathways of DAPs and DEMs. We believed that the GO terms and KEGG pathways were significantly enriched when P -value ≤ 0.05 ( Yang et al, 2018 ). In addition, we used https://www.ricedata.cn/gene/ search for DAPs related to rice agronomic traits.…”

Section: Methodsmentioning

confidence: 99%

Combining Proteomics and Metabolomics to Analyze the Effects of Spaceflight on Rice Progeny

et al. 2022

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Spaceflight is a special abiotic stress, the biological effect mechanism of which on contemporary rice has been clarified, However, its effect on offspring rice was still unclear. In order to understand the response mechanism of F2 generation plants to space flight, this study used SJ-10 recoverable satellite to carry DN423 rice seeds for 12.5 days in orbit flight. After returning to the ground, the plants were then planted to F2 generation to explore the biological effect mechanism. Our research showed that in the F2 generation of TLS, the rice plant height of the space flight group increased by 33.8%, the ear length and thousand-grain weight decreased by 9.7 and 4.6%, respectively, and the grain number per panicle increased by 6.5%. Moreover, related proteins that control changes in agronomic traits have been identified. The changes of MDA, H2O2, soluble sugar, electron leakage and antioxidant enzyme activity confirmed the stress response in F2 generation plants. ITRAQ and LC-MS technology were used to reveal the change pattern of protein levels and metabolite levels in F2 generation plants, 389 and 405 proteins were identified as differentially abundant proteins in TLS and TS, respectively. In addition, there were 124 and 125 metabolites that changed during these two periods. The proteome and metabolome result further confirmed that the F2 generation plants still retained the memory of space flight stress, and retained the memory of space flight stress through genome instability. Oxidative stress signals activated sugar signals to rebuild metabolic networks to adapt to space flight stress. The reconstruction of energy metabolism, amino acid metabolism, phenylalanine metabolism, and flavonoid metabolism played an important role in the process of adapting to space flight stress. The results of this study broaden the perspective of space biological effects and provide a basis for studying the effects of abiotic stress on plant progeny.

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iTRAQ-based proteomics screen for potential regulators of wheat (Triticum aestivum L.) root cell wall component response to Al stress

Cited by 12 publications

References 66 publications

WITHDRAWN: Lignin metabolism involved in zinc-alleviating effects on iron-induced phytotoxicity in wheat roots

WITHDRAWN: Lignin metabolism involved in zinc-alleviating effects on iron-induced phytotoxicity in wheat roots

Comparative Inter- and IntraSpecies Transcriptomics Revealed Key Differential Pathways Associated With Aluminium Stress Tolerance in Lentil

Combining Proteomics and Metabolomics to Analyze the Effects of Spaceflight on Rice Progeny

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