iTRAQ-based proteomics analysis of hippocampus in spatial memory deficiency rats induced by simulated microgravity

Wang, Tingmei; Chen, Hailong; Lv, Ke; Ji, Guohua; Zhang, Yongliang; Wang, Yanli; Li, Yinghui; Qu, Lei

doi:10.1016/j.jprot.2017.03.013

Cited by 33 publications

(33 citation statements)

References 47 publications

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“…The methods of quantitative proteomics have been widely used in the mechanistic study of traditional Chinese medicines to find disease-specific targets and biomarkers ( Li et al, 2014 ). Among them, isobaric tags for relative and absolution quantitation (iTRAQ) combining with multidimensional liquid chromatography and tandem MS analysis is one of the most powerful methodologies for investigating drug targets and molecular mechanisms ( Wang T. et al, 2017 ). Thus, iTRAQ may be a plausible way for the discovery of drug-target of dioscin on HSCs cell migration.…”

Section: Introductionmentioning

confidence: 99%

Dioscin Inhibits HSC-T6 Cell Migration via Adjusting SDC-4 Expression: Insights from iTRAQ-Based Quantitative Proteomics

Yin

et al. 2017

Front. Pharmacol.

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Hepatic stellate cells (HSCs) migration, an important bioprocess, contributes to the development of liver fibrosis. Our previous studies have found the potent activity of dioscin against liver fibrosis by inhibiting HSCs proliferation, triggering the senescence and inducing apoptosis of activated HSCs, but the molecular mechanisms associated with cell migration were not clarified. In this work, iTRAQ (isobaric tags for relative and absolution quantitation)-based quantitative proteomics study was carried out, and a total of 1566 differentially expressed proteins with fold change ≥2.0 and p < 0.05 were identified in HSC-T6 cells treated by dioscin (5.0 μg/mL). Based on Gene Ontology classification, String and KEGG pathway assays, the effects of dioscin to inhibit cell migration via regulating SDC-4 were carried out. The results of wound-healing, cell migration and western blotting assays indicated that dioscin significantly inhibit HSC-T6 cell migration through SDC-4-dependent signal pathway by affecting the expression levels of Fn, PKCα, Src, FAK, and ERK1/2. Specific SDC-4 knockdown by shRNA also blocked HSC-T6 cell migration, and dioscin slightly enhanced the inhibiting effect. Taken together, the present work showed that SDC-4 played a crucial role on HSC-T6 cell adhesion and migration of dioscin against liver fibrosis, which may be one potent therapeutic target for fibrotic diseases.

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Section: Introductionmentioning

confidence: 99%

Dioscin Inhibits HSC-T6 Cell Migration via Adjusting SDC-4 Expression: Insights from iTRAQ-Based Quantitative Proteomics

Yin

et al. 2017

Front. Pharmacol.

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“…Finally, although the impairment in memory processes in people exposed to WBV are not clearly demonstrated, 32,33 several studies in mice have suggested that alterations of spatial navigation and memory could be induced by gravity changes. 34 This is supported by transcriptomic and proteomic analysis of brain in mice models, 22,35,36 and more recently in humans by magnetic resonance imaging. 4,[37][38][39][40] As also proposed in the field of neurodegenerative disorders, an impairment of the BBB is suggested to induce or accelerate the processes of the memory impairments.…”

Section: Discussionmentioning

confidence: 93%

Effects of centrifugation and whole-body vibrations on blood–brain barrier permeability in mice

et al. 2020

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Modifications of gravity levels induce generalized adaptation of mammalian physiology, including vascular, brain, muscle, bone and immunity functions. As a crucial interface between the vascular system and the brain, the blood-brain barrier (BBB) acts as a filter to protect neurons from pathogens and inflammation. Here we compare the effects of several protocols of hypergravity induced by centrifugation and whole-body vibrations (WBV) on BBB integrity. The immunohistochemistry revealed immunoglobulin G (IgG) extravasation from blood to hippocampal parenchyma of mice centrifuged at 2 × g during 1 or 50 days, whereas short exposures to higher hypergravity mimicking the profiles of spaceflight landing and takeoff (short exposures to 5 × g) had no effects. These results suggest prolonged centrifugation (>1 days) at 2 × g induced a BBB leakage. Moreover, WBV were similarly tested. The short exposure to +2 × g vibrations (900 s/day at 90 Hz) repeated for 63 days induced IgG extravasation in hippocampal parenchyma, whereas the progressive increase of vibrations from +0.5 to +2 × g for 63 days was not able to affect the IgG crossing through the BBB. Overall, these results suggest that the BBB permeability is sensitive to prolonged external accelerations. In conclusion, we advise that the protocols of WBV and centrifugation, proposed as countermeasure to spaceflight, should be designed with progressively increasing exposure to reduce potential side effects on the BBB.

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“…Similarly, a prolonged unilateral lower limb suspension, in humans, induces a variation of the nervous system plastic properties (Clark et al, 2006 ). On the site of neurotransmitters, it was demonstrated that 28 days of HU exposure in rats induced changes in the memory function and spatial learning that were related to protein expression changes (75 proteins were over-expressed and 72 down-regulated; Wang et al, 2017 ). In particular, the expression of Glutamate receptor (GluR) 1, GluR4, and the level of glutamate were up-regulated (Wang et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…On the site of neurotransmitters, it was demonstrated that 28 days of HU exposure in rats induced changes in the memory function and spatial learning that were related to protein expression changes (75 proteins were over-expressed and 72 down-regulated; Wang et al, 2017 ). In particular, the expression of Glutamate receptor (GluR) 1, GluR4, and the level of glutamate were up-regulated (Wang et al, 2017 ). On the other hand, the concentration of serotonin, dopamine, GABA, and epinephrine was reduced respect to control rats (Wang et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Reduction of Movement in Neurological Diseases: Effects on Neural Stem Cells Characteristics

et al. 2018

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Both astronauts and patients affected by chronic movement-limiting pathologies face impairment in muscle and/or brain performance. Increased patient survival expectations and the expected longer stays in space by astronauts may result in prolonged motor deprivation and consequent pathological effects. Severe movement limitation can influence not only the motor and metabolic systems but also the nervous system, altering neurogenesis and the interaction between motoneurons and muscle cells. Little information is yet available about the effect of prolonged muscle disuse on neural stem cells characteristics. Our in vitro study aims to fill this gap by focusing on the biological and molecular properties of neural stem cells (NSCs). Our analysis shows that NSCs derived from the SVZ of HU mice had shown a reduced proliferation capability and an altered cell cycle. Furthermore, NSCs obtained from HU animals present an incomplete differentiation/maturation. The overall results support the existence of a link between reduction of exercise and muscle disuse and metabolism in the brain and thus represent valuable new information that could clarify how circumstances such as the absence of load and the lack of movement that occurs in people with some neurological diseases, may affect the properties of NSCs and contribute to the negative manifestations of these conditions.

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iTRAQ-based proteomics analysis of hippocampus in spatial memory deficiency rats induced by simulated microgravity

Cited by 33 publications

References 47 publications

Dioscin Inhibits HSC-T6 Cell Migration via Adjusting SDC-4 Expression: Insights from iTRAQ-Based Quantitative Proteomics

Dioscin Inhibits HSC-T6 Cell Migration via Adjusting SDC-4 Expression: Insights from iTRAQ-Based Quantitative Proteomics

Effects of centrifugation and whole-body vibrations on blood–brain barrier permeability in mice

Reduction of Movement in Neurological Diseases: Effects on Neural Stem Cells Characteristics

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