iTRAQ-based proteomic technique provides insights into salt stress responsive proteins in Apocyni Veneti Folium (Apocynum venetum L.)

Chen, Cuihua; Wang, Yuming; Liu, Zixiu; Cai, Zhichen; Hua, Yujiao; Mei, Yuqi; Wei, Lifang; Liu, Xunhong

doi:10.1016/j.envexpbot.2020.104247

Cited by 9 publications

(6 citation statements)

References 52 publications

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“…The WGCNA algorithm is based on the assumed gene network without scale distribution. It then constructs a gene co-expression correlation matrix and gene network, defines them as adjacency functions, and analyzes and calculates the dissimilarity coefficients of different nodes to construct a hierarchical clustering tree (Chen et al, 2020a). Cytoscape_3.7.1 was used to draw the weighted gene co-expression network graph.…”

Section: Rna Extraction Sequencing and Data Processingmentioning

confidence: 99%

Physiological and Comparative Transcriptomic Analysis Provide Insight Into Cotton (Gossypium hirsutum L.) Root Senescence in Response

et al. 2021

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Nitrogen (N) deficiency is one of the pivotal environmental factors that induce leaf senescence. However, little is known regarding the impact of low N on root senescence in cotton. Thus, the objective of this study was to investigate the effect of low nitrogen on root senescence. In this study, the molecular mechanism of cotton root senescence in response to nitrogen deficiency was investigated by combing physiological and transcriptomic analysis when no nitrogen and normal nitrogen (138mg N·kg−1 soil). The results showed that: (1) nitrogen starvation induced the premature senescence of leaf, while delaying root senescence. (2) The increase in catalase (CAT) activity at 60, 80, and 100days after emergence (DAE), combined with decrease of malonaldehyde content at 60, 80, and 100 DAE, and the content of abscisic acid (ABA), all of these contributed to the delay of root senescence by low nitrogen treatment. (3) To study the molecular mechanisms underlying root senescence, the gene expression profiling between low nitrogen and normal nitrogen treatments were compared pairwise at 20, 40, 60, 80, and 100 DAE. A total of 14,607 genes were identified to be differentially expressed at these five points. (5) Most genes involved in glutathione (GSH) and ascorbate peroxidase (APX) synthesis were upregulated, while ABA, apoptosis, caspase, and cell cycle-related differentially expressed genes (DEGs) were downregulated. Coupled with the physiology data, these results provide new insights into the effect of nitrogen starvation on root senescence.

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Section: Rna Extraction Sequencing and Data Processingmentioning

confidence: 99%

Physiological and Comparative Transcriptomic Analysis Provide Insight Into Cotton (Gossypium hirsutum L.) Root Senescence in Response

et al. 2021

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“…To gain a more comprehensive understanding, profiling experiments with translated mRNA might reveal the importance of proteomics. Proteomics aids to identifying the detailed function of differentially expressed proteins in response to stress 168,169 . It explains the mechanisms of plant stress response and growth dynamics, thereby promoting crop yield and quality.…”

Section: Employing Omics Technologies In Carbon and Nitrogen Metaboli...mentioning

confidence: 99%

The interplay of carbon and nitrogen distribution: Prospects for improved crop yields

2023

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Growth and productivity of plants primarily depend on the balanced distribution of carbon (C) and nitrogen (N) among different organs. Previous studies on crop improvement have focussed on the C or N assimilation and distribution. However, recent findings reveal that C and N form a complex integrated network and are often dependent on each other to affect crop productivity. The underlying physiological and molecular mechanisms involved in the coordinated distribution of C and N among different plant organs are yet to be fully uncovered. Crucial roles in regulating C and N balance are played by transporters that mediate their movement across different organs. In Cotton, which has an indeterminate growth pattern, source-sink assimilate distribution could be a major bottleneck impeding fibre productivity. This review summarises our current understanding of C and N transport mechanisms, explores and compares different physiological and molecular approaches involved in the C-N distribution cascade, including cotton and other plant species. A comprehensive understanding of these integrated regulatory mechanisms is crucial for improving crop yields and fibre productivity.

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“…In addition, some proteins, such as heat shock proteins (HSPs) 15 , mitogen-activated protein kinases (MAPKs) 16 , and the photosynthetic protein PSBO2 17 , are thought to be involved in a variety of signal transduction pathways related to the salt-stress response, thereby increasing resistance to abiotic stress. For instance, MAPKs play crucial roles in the regulation of transcription factor gene expression under salt stress in rice 18 .…”

Section: Introductionmentioning

confidence: 99%

Integrated physiological, proteomic, and metabolomic analyses of pecan cultivar ‘Pawnee’ adaptation to salt stress

Jiao

Zhang

Cun-de

2022

Sci Rep

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The pecan is a salt-alkali-tolerant plant, and its fruit and wood have high economic value. This study aimed to explore the molecular mechanisms responsible for salt stress tolerance in the pecan grown under hydroponic conditions to simulate salt stress. The results showed that the photosynthetic rate (Pn) was reduced in response to salt stress, while the intercellular carbon dioxide concentrations (Ci) increased. The response of the pecan to salt stress was measured using iTRAQ (isobaric tags for relative or absolute quantitation) and LC/MS (liquid chromatography and mass spectrometry) non-targeted metabolomics technology. A total of 198 differentially expressed proteins (65 down-regulated and 133 up-regulated) and 538 differentially expressed metabolites (283 down-regulated and 255 up-regulated) were identified after exposure to salt stress for 48 h. These genes were associated with 21 core pathways, shown by Kyoto Encyclopedia of Genes and Genomes annotation and enrichment, including the metabolic pathways involved in nucleotide sugar and amino sugar metabolism, amino acid biosynthesis, starch and sucrose metabolism, and phenylpropane biosynthesis. In addition, analysis of interactions between the differentially expressed proteins and metabolites showed that two key nodes of the salt stress regulatory network, L-fucose and succinate, were up-regulated and down-regulated, respectively, suggesting that these metabolites may be significant for adaptations to salt stress. Finally, several key proteins were further verified by parallel reaction monitoring. In conclusion, this study used physiological, proteomic, and metabolomic methods to provide an important preliminary foundation for improving the salt tolerance of pecans.

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iTRAQ-based proteomic technique provides insights into salt stress responsive proteins in Apocyni Veneti Folium (Apocynum venetum L.)

Cited by 9 publications

References 52 publications

Physiological and Comparative Transcriptomic Analysis Provide Insight Into Cotton (Gossypium hirsutum L.) Root Senescence in Response

Physiological and Comparative Transcriptomic Analysis Provide Insight Into Cotton (Gossypium hirsutum L.) Root Senescence in Response

The interplay of carbon and nitrogen distribution: Prospects for improved crop yields

Integrated physiological, proteomic, and metabolomic analyses of pecan cultivar ‘Pawnee’ adaptation to salt stress

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