iTRAQ-based proteomic analysis of the rat striatum in response to methamphetamine preconditioning

Lu, Shuang; Yang, Yandi; Liao, Lvshuang; Yan, Weitao; Xiong, Kun; Yan, Jie

doi:10.1093/abbs/gmab024

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…DA cell bodies in the midbrain are the primary source of dopaminergic input to the caudate putamen (CP) and nucleus accumbens (NAc), and DA neurons in the ventral tegmental area (VTA) and the substantia nigra (SN) send their axons to the NAc and CP, respectively ( Lammel et al, 2014 ; Thanos et al, 2016 ). DA is the major neurotransmitter in the neostriatum ( Björklund and Dunnett, 2007 ), and neural activity of DA neurons in the somatodendritic field may influence the synthesis and release of DA in the terminal regions ( Poulin et al, 2020 ; Lu et al, 2021 ). In vitro studies have shown enhanced DA release from NAc and striatum tissue slices from rats sensitized to psychostimulants such as MA ( Desai et al, 2010 ; Granado et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Evidence for Dopamine Abnormalities Following Acute Methamphetamine Exposure Assessed by Neuromelanin-Sensitive Magnetic Resonance Imaging

Tang

Liu

Zhang

et al. 2022

Front. Aging Neurosci.

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BackgroundNeuromelanin-sensitive magnetic resonance imaging (NM-MRI) is a newly developed MRI technique that provides a non-invasive way to indirectly measure of dopamine (DA) function. This study aimed to determine NM concentrations in brain regions following acute methamphetamine (MA) administration using NM-MRI and to explore whether NM-MRI can be used as a biomarker of DA function in non-neurodegenerative diseases.MethodsBaseline NM-MRI, T1-weighted and T2-weighted images were acquired from 27 rats before drug/placebo injection. The control group (n = 11) received acute placebo (Normal saline), while the experimental group (n = 16) received acute MA. NM-MRI scans were performed 5, 30, 60 and 90 min after injection. Regions of interest (ROIs), including the caudate putamen (CP), nucleus accumbens (NAc), hippocampus (HIP), substantia nigra (SN) and crus cerebri (CC), were manually drawn by an experienced radiologist. NM-MRI signal intensity in five brain regions at different time points (baseline and 5, 30, 60, and 90 min) were analyzed.ResultsIn both the control and experimental groups, at each time point (baseline and 5, 30, 60, and 90 min), the SN exhibited significantly higher NM-MRI signal intensity than the other brain regions (P < 0.05). In addition, acute MA administration resulted in a continuous upward trend in NM-MRI signal intensity in each brain region over time. However, there was no such trend over time in the control group. The NM-MRI signal intensity of SN in the experimental group was significantly higher at the 60 and 90 min compared with that in the control group (P values were 0.042 and 0.042 respectively). Within experimental group, the NM-MRI signal intensity of SN was significantly higher at the 60 and 90 min compared with that before MA administration (P values were 0.023 and 0.011 respectively). Increased amplitudes and rates of NM-MRI signal intensity were higher in the SN than in other brain regions after MA administration.ConclusionOur results indicated that NM was mainly deposited in the SN, and the conversion of DA to NM was most significant in the SN after acute MA exposure. Increased DA release induced by acute MA exposure may lead to increased accumulation of NM in multiple brain regions that can be revealed by NM-MRI. NM-MRI may serve as a powerful imaging tool that could have diverse research and clinical applications for detecting pathological changes in drug addiction and related non-neurodegenerative diseases.

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Section: Introductionmentioning

confidence: 99%

Evidence for Dopamine Abnormalities Following Acute Methamphetamine Exposure Assessed by Neuromelanin-Sensitive Magnetic Resonance Imaging

Tang

Liu

Zhang

et al. 2022

Front. Aging Neurosci.

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“…They also play essential roles in stimulating and maintaining movement, emotional control, reward effect, and drug dependence ( Alexander et al, 1986 ; Calabresi et al, 1997 ). Unfortunately, striatal neurons are very sensitive to METH-induced neurotoxicity ( Granado et al, 2010 ; Yamamoto et al, 2010 ; Valian et al, 2017 ; Granado et al, 2018 ; Lu et al, 2021 ). Studies have shown the degeneration of dopaminergic terminals and the death of cell bodies in the striatum following METH treatment ( Zhu et al, 2009 ; Ares-Santos et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

The Role of HSP90α in Methamphetamine/Hyperthermia-Induced Necroptosis in Rat Striatal Neurons

Liao

Yan

et al. 2021

Front. Pharmacol.

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Methamphetamine (METH) is one of the most widely abused synthetic drugs in the world. The users generally present hyperthermia (HT) and psychiatric symptoms. However, the mechanisms involved in METH/HT-induced neurotoxicity remain elusive. Here, we investigated the role of heat shock protein 90 alpha (HSP90α) in METH/HT (39.5°C)-induced necroptosis in rat striatal neurons and an in vivo rat model. METH treatment increased core body temperature and up-regulated LDH activity and the molecular expression of canonical necroptotic factors in the striatum of rats. METH and HT can induce necroptosis in primary cultures of striatal neurons. The expression of HSP90α increased following METH/HT injuries. The specific inhibitor of HSP90α, geldanamycin (GA), and HSP90α shRNA attenuated the METH/HT-induced upregulation of receptor-interacting protein 3 (RIP3), phosphorylated RIP3, mixed lineage kinase domain-like protein (MLKL), and phosphorylated MLKL. The inhibition of HSP90α protected the primary cultures of striatal neurons from METH/HT-induced necroptosis. In conclusion, HSP90α plays an important role in METH/HT-induced neuronal necroptosis and the HSP90α-RIP3 pathway is a promising therapeutic target for METH/HT-induced neurotoxicity in the striatum.

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Do pyroptosis, apoptosis, and necroptosis (PANoptosis) exist in cerebral ischemia? Evidence from cell and rodent studies

Yan

Yang

et al. 2022

Neural Regen Res

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iTRAQ-based proteomic analysis of the rat striatum in response to methamphetamine preconditioning

Cited by 3 publications

References 10 publications

Evidence for Dopamine Abnormalities Following Acute Methamphetamine Exposure Assessed by Neuromelanin-Sensitive Magnetic Resonance Imaging

Evidence for Dopamine Abnormalities Following Acute Methamphetamine Exposure Assessed by Neuromelanin-Sensitive Magnetic Resonance Imaging

The Role of HSP90α in Methamphetamine/Hyperthermia-Induced Necroptosis in Rat Striatal Neurons

Do pyroptosis, apoptosis, and necroptosis (PANoptosis) exist in cerebral ischemia? Evidence from cell and rodent studies

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