iTRAQ-Based Comparative Proteomic Analysis of Adult Schistosoma japonicum from Water Buffalo and Yellow Cattle

Zhai, Qixiao; Fu, Zhiqiang; Hong, Yang; Yu, Xianjun; Han, Qian; Ke, Lu; Li, Hao; Dou, Xuefeng; Zhu, Chuangang; Lin, Jiaojiao; Li, Guoqing

doi:10.3389/fmicb.2018.00099

Cited by 15 publications

(18 citation statements)

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“…Reactive oxygen species are found in normal living organisms where they are constantly being produced under the oxidative stress caused by toxic heavy metals, heat shock, inflammation, ionizing irradiation, immune responses, and environmental stimuli ( Zhai et al, 2018 ). Studies have shown that antioxidant enzymes can remove and reduce ROS produced by metabolic stress conditions in an attempt to maintain homeostatic equilibrium.…”

Section: Discussionmentioning

confidence: 99%

iTRAQ-Based Quantitative Proteomic Analysis Reveals Proteomic Changes in Mycelium of Pleurotus ostreatus in Response to Heat Stress and Subsequent Recovery

Zou

Zhang

et al. 2018

Front. Microbiol.

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High temperature is a key limiting factor for mycelium growth and development in Pleurotus ostreatus. Thermotolerance includes the direct response to heat stress and the ability to recover from heat stress. To better understand the mechanism of thermotolerance in P. ostreatus, we used morphological and physiological analysis combined with an iTRAQ-based proteomics analysis of P. ostreatus subjected to 40°C for 48 h followed by recovery at 25°C for 3 days. High temperature increased the concentrations of thiobarbituric acid reactive substances (TBARS) indicating that the mycelium of P. ostreatus were damaged by heat stress. However, these physiological changes rapidly returned to control levels during the subsequent recovery phase from heat stress. In comparison to unstressed controls, a total of 204 proteins were changed during heat stress and/or the recovery phase. Wherein, there were 47 proteins that responded to both stress and recovery conditions, whereas 84 and 73 proteins were responsive to only heat stress or recovery conditions, respectively. Furthermore, quantitative real-time PCR (qRT-PCR) confirmed differential expression of nine candidate genes revealed that some of the proteins, such as a mitogen-activated protein kinase (MAPK), phenylalanine ammonia-lyase (PAL), and heat shock protein (HSP), were also regulated by heat stress at the level of transcription. These differentially expressed proteins (DEPs) in mycelium of P. ostreatus under heat stress were from 13 biological processes. Moreover, protein–protein interaction analysis revealed that proteins involved in carbohydrate and energy metabolism, signal transduction, and proteins metabolism could be assigned to three heat stress response networks. On the basis of these findings, we proposed that effective regulatory protein expression related to MAPK-pathway, antioxidant enzymes, HSPs, and other stress response proteins, and glycolysis play important roles in enhancing P. ostreatus adaptation to and recovery from heat stress. Of note, this study provides useful information for understanding the thermotolerance mechanism for basidiomycetes.

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Section: Discussionmentioning

confidence: 99%

iTRAQ-Based Quantitative Proteomic Analysis Reveals Proteomic Changes in Mycelium of Pleurotus ostreatus in Response to Heat Stress and Subsequent Recovery

Zou

Zhang

et al. 2018

Front. Microbiol.

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“…Eukaryotic initiation factor 4A is a highly conserved RNA-stimulated ATPase and helicase involved in the initiation of mRNA translation, which has been found to play an important role in ovule development and cell size homeostasis in Arabidopsis [77]. However, the available information was limited to make further correlation analysis between differentially expressed miRNAs and the differentially expressed proteins of worms derived from yellow cattle and water buffalo [16]. A total of 131 differentially expressed proteins were identified between worms from yellow cattle and water buffalo in our previous study, but only 49 differentially expressed proteins were annotated in the known database [16].…”

Section: Discussionmentioning

confidence: 99%

“…PCR amplification was performed with a SYBR ® PremixEx Taq™ kit (TaKaRa, Dalian, China) on an ABI 7500 Real-Time System (Applied Biosystems). The reaction conditions were as follows: 95 °C for 30 s, 40 cycles of 95 °C for 5 s and 60 °C for 34 s. Sj NADH was selected as an internal reference [16]. The target gene numbers and primer sequences are shown in Additional file 4: Table S3.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, the surface topography and internal structures at the ultrastructural level have also been found to differ between adult worms isolated from water buffalo and from yellow cattle [15]. Recent studies in our laboratory have revealed that adult S. japonicum from yellow cattle and water buffalo displays different expression profiles at the transcript and protein levels [15, 16]. The expression of some functional proteins is closely correlated with the development of schistosomes, and is required for growth, reproduction, energy metabolism and evasion of the host immune response.…”

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of microRNA expression profiles of adult Schistosoma japonicum isolated from water buffalo and yellow cattle

Zhai

et al. 2019

Parasites Vectors

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Background Yellow cattle and water buffalo are important natural reservoir hosts and the main transmission sources of Schistosoma japonicum in endemic areas of China. The worms from the two hosts have marked differences in general worm morphology and ultrastructure, gene transcription and protein expression profiles. Results To investigate microRNAs (miRNAs) involved in the regulation of schistosome development and survival, we compared miRNA expression profiles of adult schistosomes derived from yellow cattle and water buffalo by using high-throughput sequencing with Illumina Hiseq Xten. Schistosoma japonicum from water buffalo and yellow cattle yielded 63.78 million and 63.21 million reads, respectively, of which nearly 50% and 49% could be mapped to selected miRNAs in miRbase. A total of 206 miRNAs were identified, namely 79 previously annotated miRNAs of S. japonicum and 127 miRNAs that matched with the S. japonicum genome and were highly similar to the annotated miRNAs from other organisms. Among the 79 miRNAs, five (sja-miR-124-3p, sja-miR-219-5p, sja-miR-2e-3p, sja-miR-7-3p and sja-miR-3490) were significantly upregulated in the schistosomes from water buffalo compared with those from yellow cattle. A total of 268 potential target genes were predicted for these five differentially expressed miRNAs. Eleven differentially expressed targets were confirmed by qRT-PCR among 15 tested targets, one of which was further validated through dual-luciferase reporter assay. Among the 127 ‘possible’ S. japonicum miRNAs, ten were significantly differentially expressed in the schistosomes from these two hosts. Conclusions These results highlight the important roles of miRNAs in regulating the development and survival of schistosomes in water buffalo and yellow cattle and facilitate understanding of the miRNA regulatory mechanisms in schistosomes derived from different susceptible hosts. Electronic supplementary material The online version of this article (10.1186/s13071-019-3450-7) contains supplementary material, which is available to authorized users.

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“…Numerous researchers had endeavored to explore the genes participating or involved in the regulation of the development and reproductive maturation of schistosomes by comparative transcriptome, proteome, and metabolome research and gained great discoveries in the growth and reproductive biology of schistosomes (Gupta and Basch, 1987;Haseeb et al, 1989;Siegel and Tracy, 1989;Haseeb and Eveland, 1991;Grevelding et al, 1997;Haseeb, 1998;Liu et al, 2006;Cogswell et al, 2012;Leutner et al, 2013;Protasio et al, 2013;Sun et al, 2014;Han et al, 2015a,b;Cao et al, 2016;Picard et al, 2016;Zhai et al, 2018;Li et al, 2019;Mao et al, 2019). Recently, we have also investigated and reported the differential metabolomic profiles of S. japonicum parasites from SCID and BALB/c mice in order to explore the molecular events involved in the aberrant morphologies in the growth and reproduction of schistosomes from a metabolomic level.…”

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confidence: 99%

Comparative Transcriptome Analyses of Schistosoma japonicum Derived From SCID Mice and BALB/c Mice: Clues to the Abnormality in Parasite Growth and Development

Liu¹,

Cheng²,

Ye³

et al. 2020

Front. Microbiol.

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Frontiers in Microbiology | www.frontiersin.org March 2020 | Volume 11 | Article 274 Liu et al.Transcriptome Analysis of S. japonicum From Mice expression profiles of schistosomes from SCID mice when compared with those from BALB/c mice, which were potentially involved in regulating the growth and development of schistosomes. These findings contributed to an understanding of parasite biology and provided a rich resource for the exploitation of antischistosomal intervention targets.

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iTRAQ-Based Comparative Proteomic Analysis of Adult Schistosoma japonicum from Water Buffalo and Yellow Cattle

Cited by 15 publications

References 80 publications

iTRAQ-Based Quantitative Proteomic Analysis Reveals Proteomic Changes in Mycelium of Pleurotus ostreatus in Response to Heat Stress and Subsequent Recovery

iTRAQ-Based Quantitative Proteomic Analysis Reveals Proteomic Changes in Mycelium of Pleurotus ostreatus in Response to Heat Stress and Subsequent Recovery

Comparative analysis of microRNA expression profiles of adult Schistosoma japonicum isolated from water buffalo and yellow cattle

Comparative Transcriptome Analyses of Schistosoma japonicum Derived From SCID Mice and BALB/c Mice: Clues to the Abnormality in Parasite Growth and Development

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