ITO-coated glass/polydimethylsiloxane continuous-flow PCR chip

Joung, Seung-Ryong; Kim, Jaewan; Choi, Young Jin; Kang, Chi Jung; Yong‐Sang, Kim

doi:10.1109/nems.2007.352113

Cited by 5 publications

(5 citation statements)

References 6 publications

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“…In addition to conventional glass material, indium tin oxide (ITO)—a transparent and colorless material in thin film—has been widely applied in microfluidic devices because of its optical transparency and electrical conductivity characteristics. The electrical conductivity of ITO films enables the widespread production of electrophoresis or dielectrophoresis biochips in polymerase chain reaction (PCR) or particle/cell screening applications [ 12 , 13 ]. Ghrera et al [ 14 ] used the chemical etching method to produce a pattern on ITO-coated glass and seal it with PDMS to fabricate the microchannel.…”

Section: Introductionmentioning

confidence: 99%

Surface Wettability and Electrical Resistance Analysis of Droplets on Indium-Tin-Oxide Glass Fabricated Using an Ultraviolet Laser System

Tsai

Hsu

et al. 2021

Micromachines

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Indium tin oxide (ITO) is widely used as a substrate for fabricating chips because of its optical transparency, favorable chemical stability, and high electrical conductivity. However, the wettability of ITO surface is neutral (the contact angle was approximately 90°) or hydrophilic. For reagent transporting and manipulation in biochip application, the surface wettability of ITO-based chips was modified to the hydrophobic or nearly hydrophobic surface to enable their use with droplets. Due to the above demand, this study used a 355-nm ultraviolet laser to fabricate a comb microstructure on ITO glass to modify the surface wettability characteristics. All of the fabrication patterns with various line width and pitch, depth, and surface roughness were employed. Subsequently, the contact angle (CA) of droplets on the ITO glass was analyzed to examine wettability and electrical performance by using the different voltages applied to the electrode. The proposed approach can succeed in the fabrication of a biochip with suitable comb-microstructure by using the optimal operating voltage and time functions for the catch droplets on ITO glass for precision medicine application. The experiment results indicated that the CA of droplets under a volume of 20 μL on flat ITO substrate was approximately 92° ± 2°; furthermore, due to its lowest surface roughness, the pattern line width and pitch of 110 μm exhibited a smaller CA variation and more favorable spherical droplet morphology, with a side and front view CA of 83° ± 1° and 78.5° ± 2.5°, respectively, while a laser scanning speed of 750 mm/s was employed. Other line width and pitch, as well as scanning speed parameters, increased the surface roughness and resulted in the surface becoming hydrophilic. In addition, to prevent droplet morphology collapse, the droplet’s electric operation voltage and driving time did not exceed 5 V and 20 s, respectively. With this method, the surface modification process can be employed to control the droplet’s CA by adjusting the line width and pitch and the laser scanning speed, especially in the neutral or nearly hydrophobic surface for droplet transporting. This enables the production of a microfluidic chip with a surface that is both light transmittance and has favorable electrical conductivity. In addition, the shape of the microfluidic chip can be directly designed and fabricated using a laser direct writing system on ITO glass, obviating the use of a mask and complicated production processes in biosensing and biomanipulation applications.

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Section: Introductionmentioning

confidence: 99%

Surface Wettability and Electrical Resistance Analysis of Droplets on Indium-Tin-Oxide Glass Fabricated Using an Ultraviolet Laser System

Tsai

Hsu

et al. 2021

Micromachines

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“…As a result, in microfluidic technology, the materials used to develop microfluidic devices are quite crucial. In general, microfluidic devices can be made from a variety of materials, such as silicone [ 108 ], glass [ 109 ], paper [ 110 ], graphene [ 111 ], polydimethylsiloxane (PDMS) [ 112 ], and polymethylmethacrylate (PMMA) [ 113 ]. Table 6 showcases several materials utilized to develop microfluidic biosensors.…”

Section: Methodsmentioning

confidence: 99%

Biosensors and Microfluidic Biosensors: From Fabrication to Application

2022

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Biosensors are ubiquitous in a variety of disciplines, such as biochemical, electrochemical, agricultural, and biomedical areas. They can integrate various point-of-care applications, such as in the food, healthcare, environmental monitoring, water quality, forensics, drug development, and biological domains. Multiple strategies have been employed to develop and fabricate miniaturized biosensors, including design, optimization, characterization, and testing. In view of their interactions with high-affinity biomolecules, they find application in the sensitive detection of analytes, even in small sample volumes. Among the many developed techniques, microfluidics have been widely explored; these use fluid mechanics to operate miniaturized biosensors. The currently used commercial devices are bulky, slow in operation, expensive, and require human intervention; thus, it is difficult to automate, integrate, and miniaturize the existing conventional devices for multi-faceted applications. Microfluidic biosensors have the advantages of mobility, operational transparency, controllability, and stability with a small reaction volume for sensing. This review addresses biosensor technologies, including the design, classification, advances, and challenges in microfluidic-based biosensors. The value chain for developing miniaturized microfluidic-based biosensor devices is critically discussed, including fabrication and other associated protocols for application in various point-of-care testing applications.

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“…Thus, the selection and invention of substrates hold paramount importance in microfluidic manufacturing technologies. In a nutshell, the successful evolution of technology has incorporated an extensive array of materials for microfluidic device fabrication, encompassing, but not restricted to, silicone rubber [31], glass [32], paper [33], graphene [34], PDMS [35], and PMMA [36].…”

Section: Miniaturized Microfluidic Biosensors: Design and Fabricationmentioning

confidence: 99%

Microfluidic biosensors: exploring various applications through diverse bonding methods

Yang,

Zhu

2024

J. Micromech. Microeng.

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Biological sensors are widely applied in agriculture, biomedicine, food, healthcare, environmental monitoring, water quality, forensics, drug development, etc.. Particularly the utilization of microfluidic technology has become prevalent in the development and manufacturing of biosensors for miniaturization, automation, and integration. Microfluidic biosensors have distinct advantages, including enhanced diffusive timescales, controlled concentration gradients, high throughput, high precision fluid manipulation, stable reaction environments and high sensitivity. From the perspective of sensor fabrication, bonding remains the crucial pathway in the pursuit of integrating microfluidic technology with biosensor chips, while various bonding methods are employed across different application domains. This paper delves into the classification, progress, and challenges associated with these bonding methods corresponding with various microfluidic biosensors in diverse applications. The review presented herein highlights the latest advancements in microfluidic biosensors based on diverse bonding methods, underscoring their significant application prospects and developmental potential within these fields.

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ITO-coated glass/polydimethylsiloxane continuous-flow PCR chip

Cited by 5 publications

References 6 publications

Surface Wettability and Electrical Resistance Analysis of Droplets on Indium-Tin-Oxide Glass Fabricated Using an Ultraviolet Laser System

Surface Wettability and Electrical Resistance Analysis of Droplets on Indium-Tin-Oxide Glass Fabricated Using an Ultraviolet Laser System

Biosensors and Microfluidic Biosensors: From Fabrication to Application

Microfluidic biosensors: exploring various applications through diverse bonding methods

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