2000
DOI: 10.1111/j.1365-2818.2000.00653.x
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Iterative thresholding for segmentation of cells from noisy images

Abstract: We introduce an iterative thresholding algorithm for the segmentation of cells from noisy cell images. The thresholding image, which is initially a constant, changes iteratively with both the previous segmentation and image local activity. Experimental results for both synthesized and real cell images are provided to demonstrate the performance of the algorithm.

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Cited by 32 publications
(25 citation statements)
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“…Those methods can be broadly classified as edge-based [15], region-based [16], threshold-based [17], and watershed-based [18,19] segmentation schemes. Wu et al [15] stated that cell boundaries are not sharp enough to perform edge-based segmentation in leukocyte images.…”
Section: Literature Surveymentioning
confidence: 99%
“…Those methods can be broadly classified as edge-based [15], region-based [16], threshold-based [17], and watershed-based [18,19] segmentation schemes. Wu et al [15] stated that cell boundaries are not sharp enough to perform edge-based segmentation in leukocyte images.…”
Section: Literature Surveymentioning
confidence: 99%
“…However, cell segmentation is still a problem due to the complexities of cell structures resulting from inconsistent staining, poor contrast, and overlapping cells [1]. Global approaches such as thresholding, clustering, and histogram-based methods lead to unsatisfactory results due to the variable staining existing even within a single cell [2]. Such problems can be partially avoided using local information by exploiting local relative changes to find the boundaries between different cell structures.…”
Section: Introductionmentioning
confidence: 99%
“…They include watershed [5], [6], [7], region-based [8] and threshold-based methods [9]. Application of active contour has been widely investigated for cell segmentation [10], [11].…”
Section: Introductionmentioning
confidence: 99%