Italian multicentre study on microbial environmental contamination in dental clinics: a pilot study

Pasquarella, Cesira; Veronesi, Licia; Castiglia, Paolo; Liguori, Giorgio; Montagna, Maria Teresa; Napoli, Christian; Rizzetto, Rolando; Torre, Ida; Masia, Maria Dolores; Onofrio, Valeria Di; Colucci, Maria Eugenia; Tinteri, Carola; Tanzi, M. L.

doi:10.1016/j.scitotenv.2010.05.010

Cited by 44 publications

(39 citation statements)

References 36 publications

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“…Some of the most important factors deciding the microbial contamination or quality of water include a very small lumen size (0.5-2 mm), extremely long distribution tubing (up to 10 m), frequent irregular stagnation of the incoming water, and biofi lm formation. Microorganisms found in such environments are conditionally pathogenic bacteria, microscopic fi lamentous fungi, and various protozoa essential to the survival of Legionella (7,8,9). Therefore, it is necessary to establish the microbial diversity of microorganisms in the incoming water, and accordingly select the best biocides (10,11).…”

Section: Introductionmentioning

confidence: 99%

Legionella spp. in dental unit waterlines

Jurásková¹,

Sedlackova²,

Janska³

et al. 2017

BLL

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OBJECTIVE: To determine the current presence of Legionella spp. in the output water of dental unit waterlines (DUWLs) and examine its mitigation by disinfection at the Institute of Dentistry and Oral Sciences, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc. MATERIAL AND METHODS: The fi rst stage of our survey involved collecting samples of DUWL output water from 50 dental chair units (DCUs), and 2 samples of the incoming potable water. In October 2015, a one-time disinfection (1 % Stabimed) of DUWLs was conducted. This was followed by collecting 10 control samples (survey stage 2). RESULTS: From the total of 50 samples (survey stage 1), 18 samples (36.0 %) tested positive for Legionella spp. Following the disinfection, nine of the ten samples no longer showed any presence of Legionella. CONCLUSION: Based on culture results, the one-time disinfection (1 % Stabimed) was effective. We are unable to comment on the duration of positive effect of disinfection on the occurrence of Legionella spp. in the outlet water. It was a one-time survey (Tab. 2, Ref. 32). Text in PDF www.elis.sk. KEY WORDS: Legionella spp., water of dental unit waterlines, disinfection.

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Section: Introductionmentioning

confidence: 99%

Legionella spp. in dental unit waterlines

Jurásková¹,

Sedlackova²,

Janska³

et al. 2017

BLL

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“…En estudios más recientes, realizados en Italia, se encontró que en el agua de las unidades se obtuvieron recuentos de 24 X104 UFC/mL (36).…”

Section: Bacterias Presentes En El Agua De Las Unidades Dentalesunclassified

Calidad del Agua de Unidades Odontológicas

Navia

Torres

2012

nova

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RESUMENEl agua es una fuente importante de contaminación en pacientes odontológicos, esta contaminación se ha clasificado en dos clases: la primera, proveniente del suministro primario de agua, es decir, del agua pública y, la segunda, la que se encuentra adherida a las paredes de las mangueras de la unidad odontológica formando una biocapa, que se forma por los fluidos orales que son aspirados durante el procedimiento. La biocapa estará continuamente contaminando las mangueras de la unidad y expulsando microorganismos a la cavidad oral del paciente donde se generan aerosoles que pueden contaminar el ambiente, las superficies, los instrumentos y al personal de salud, lo que representa un riesgo en salud pública. En Colombia, no existen reportes oficiales ni datos que permitan controlar este problema de salud pública, por lo que los riesgos de contaminación e infección bacteriana siguen siendo latentes en la actualidad.Palabras clave: odontología, infecciones oportunistas, microbiología del agua, salud pública. Water quality of dental units ABSTRACTWater is a main source of contamination in dental patients, classified into two classes: the first from the primary water supply, (ie,public water) and the second one, which is attached to the hoses walls of the dental unit, forming a biofilm. Due to stagnation of oral fluids are aspirated during the procedure.The biofilm will be continuously polluting the hoses from the unit and expelling microorganisms to the patient's oral cavity where they generate aerosols that can contaminate the environment, surfaces, instruments and health personnel, which is a public health risk. In Colombia there are not official reports or data to control this public health problem, so the risk of bacterial contamination and infection are still latent today.

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“…Dental health care students and professionals are at a risk of diseases including HBV, HCV, herpes simplex virus type 1, HIV, influenza, rubella, and besides that, fixed dental units, water lines and handpieces can become vehicles of cross-infection in dental offices [3][4][5] . The main bacteria that can cause infection hazards linked to dental practice are Streptococcus pneumoniae, Mycobacterium tuberculosis, Klebsiella pneumoniae, Escherichia coli, Legionella pneumophila and Pseudomonas aeruginosa 6,7 . This aspect is critical in the dental field, where there is constant daily exposure to a wide variety of microorganisms of the oral microflora of patients 8 .…”

Section: Introductionmentioning

confidence: 99%

“…During a dental appointment, there is dispersion of splashes and aerosols containing pathogenic microorganisms that can be transmitted through saliva, blood and oral secretions onto countertops and materials, furniture and the dental unit itself 6 . Aerosols can be a source of infection for dentists and an indirect cause of occupational hazards at work 7 . To avoid infection in the oral cavity, cleaning methods or disinfection and sterilization of the dental material to be used in the patient are recommended 8 .…”

Section: Introductionmentioning

confidence: 99%

Microbial contamination of a University dental clinic in Brazil

et al. 2017

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Pathogens of the oral cavity of a patient can be transferred to the dental office surfaces by direct contact, aerosol instruments and blood or saliva. The objective of this study was to investigate the microbiological contamination presents in the stands, chairs and spittoons in the University Nilton Lins dental clinics, in Manaus, Amazonas. Samples were collected with sterile swabs and seeded in different microbiological culture media for the isolation of microorganisms collected from each room. Then, assays were carried out for identification of strains isolated from each environment, such as: Gram stain, DNA purification, Amplification of 16s rRNA genes and sequencing. All these experiments were performed in the LBS / ILMD / FIOCRUZ. It was found 40 CFU / mL in the stands, 43 on the chairs and 47 in the spittoons and it was also possible to identify microorganisms like Klebsiella pneumoniae, Shigella sonnei and Staphylococcus aureus. The greatest number of CFUs was found in Clinic 3 and it was observed that the spittoon was the dental surface with the highest number of CFUs. Some of the bacterial species isolated are opportunists, suggesting that more severe biosecurity measures must be taken in order to prevent cross-infection.

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Italian multicentre study on microbial environmental contamination in dental clinics: a pilot study

Cited by 44 publications

References 36 publications

Legionella spp. in dental unit waterlines

Legionella spp. in dental unit waterlines

Calidad del Agua de Unidades Odontológicas

Microbial contamination of a University dental clinic in Brazil

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