It’s Gut Check Time! A Universal Food Immunomarking Technique for Studying Arthropod Feeding Activities

Hagler, James R.

doi:10.1093/aesa/say040

Cited by 13 publications

(8 citation statements)

References 69 publications

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“…In particular, there is a drastic morphological change in the wing and mouthpart morphology at the adult stage, which might affect L. delicatula dispersal and flight tendency [35][36][37], as well as feeding [25]. Additionally, combining DNA barcoding with other techniques, such as stable isotope analysis [38] and/or the immunomarking technique [39], might provide interesting insights into feeding behavior of L. delicatula.…”

Section: Practical Applications Methodological Recommendations and mentioning

confidence: 99%

Use of Molecular Gut Content Analysis to Decipher the Range of Food Plants of the Invasive Spotted Lanternfly, Lycorma delicatula

Avanesyan

Lamp

2020

Insects

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Spotted lanternfly, Lycorma delicatula (Hemiptera: Fulgoridae), is an introduced highly invasive insect pest in the US that poses a significant risk to forestry and agriculture. Assessing and predicting plant usage of the lanternfly has been challenging, and little is known regarding the lanternfly nymph association with its host plants. In this study, we focused on: (a) providing a protocol for using molecular markers for food plant identification of L. delicatula; (b) determining whether the ingested plant DNA corresponds with DNA of the plants from which the lanternfly was collected; and, (c) investigating the spectrum of ingested plants. We utilized gut contents of third and fourth instar nymphs that were collected from multiple plants; we isolated ingested plant DNA and identified consumed plants. We demonstrated that (a) up to 534 bp of the rbcL gene from ingested plants can be detected in L. delicatula guts, (b) ingested plants in ~93% of the nymphs did not correspond with the plants from which the nymphs were collected, and (c) both introduced and native plants, as well as woody and non-woody plants, were ingested. This information will aid effective the monitoring and management of the lanternfly, as well as predict the lanternfly host plants with range expansion.

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Section: Practical Applications Methodological Recommendations and mentioning

confidence: 99%

Use of Molecular Gut Content Analysis to Decipher the Range of Food Plants of the Invasive Spotted Lanternfly, Lycorma delicatula

Avanesyan

Lamp

2020

Insects

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“…Enzyme-linked immunosorbent assays (ELISA) are a MGCA tool that can be used to track the spatial movement of trophic resources. Hagler [ 37 ] tagged prey with specific proteins (typically vertebrate egg protein) and the gut of the focal arthropod was tested for the presence of protein-marked food items via a standardized protein-specific ELISA. For example, the dispersal of natural enemies from buckwheat to grapes provided evidence that buckwheat trophic interactions are connected to grape production [ 38 ].…”

Section: Resultsmentioning

confidence: 99%

“…In addition, in order to track predators in space and time, ELISA is useful for monitoring the effects of trap crops, as well as to identify predation at specific pest life-stages [ 39 ]. ELISA has proven to be a valuable tool for tracking the movement of predators and prey, and is very cost effective [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

Identifying Molecular-Based Trophic Interactions as a Resource for Advanced Integrated Pest Management

Schmidt

Acebes‐Doria

Blaauw

et al. 2021

Insects

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Biodiversity is an essential attribute of sustainable agroecosystems. Diverse arthropod communities deliver multiple ecosystem services, such as biological control, which are the core of integrated pest management programs. The molecular analysis of arthropod diets has emerged as a new tool to monitor and help predict the outcomes of management on the functioning of arthropod communities. Here, we briefly review the recent molecular analysis of predators and parasitoids in agricultural environments. We focus on the developments of molecular gut content analysis (MGCA) implemented to unravel the function of community members, and their roles in biological control. We examine the agricultural systems in which this tool has been applied, and at what ecological scales. Additionally, we review the use of MGCA to uncover vertebrate roles in pest management, which commonly receives less attention. Applying MGCA to understand agricultural food webs is likely to provide an indicator of how management strategies either improve food web properties (i.e., enhanced biological control), or adversely impact them.

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“…Mollot et al (2014) proposed that the eggs might have been fed to and consumed by the larvae rather than by the foraging workers. Metabarcoding also does not enable researchers to discriminate between predation, scavenging and secondary predation (Hagler, 2019).…”

Section: Accurate Assessment Of C Sordidus Abundance Damage To Thmentioning

confidence: 99%

The challenge of biological control of Cosmopolites sordidus Germar (Col. Curculionidae): A review

Tresson

Tixier

Puech

et al. 2021

J Applied Entomology

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With growing human populations in Africa, Asia and South America, securing food production in tropical areas is increasingly important. Banana ranks 2nd in fruit production worldwide (FAO, 2018) and along with plantains is an essential component of the nutrition of a large population. The most important pest of banana and plantain is the banana root borer Cosmopolites sordidus (Gold et al., 2001; Okolle et al., 2009). Females of this weevil lay eggs in the lower pseudostem and corm, and larvae develop in the corm. This weakens the plant and can lead to dramatic losses, both directly because of toppling and indirectly because of lower yields and increased infections by plant pathogens (Gold et al., 2001). Damage caused by C. sordidus is difficult to assess, however, because the immature stages of the weevil are hidden in the pseudostem and corm and their detection requires destructive sampling (Vilardebo, 1973). Furthermore, because the damage is not immediately visible and because C. sordidus effects are partly indirect, the full extent of C. sordidus damage is often underestimated until toppling occurs. The banana weevil can be described as a K-selected species (Pianka, 1970), with a long holometabolan development (almost two months; Vinatier et al., 2009), a low fecundity (two eggs per week; Vinatier et al., 2009) and an extended lifespan (up to 4 years (Gold et al., 2001)). With these features, C. sordidus differs from pests like aphids, flies or moths that are typically rselected species that generate damage because of their ability to rapidly produce large numbers of offspring (Williamson, 1996). However, K-selected species may also be important pests (Duyck et al., 2007). Its K-selected characteristics should have consequences for the biological control of C. sordidus. Because of its slow rate of

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It’s Gut Check Time! A Universal Food Immunomarking Technique for Studying Arthropod Feeding Activities

Cited by 13 publications

References 69 publications

Use of Molecular Gut Content Analysis to Decipher the Range of Food Plants of the Invasive Spotted Lanternfly, Lycorma delicatula

Use of Molecular Gut Content Analysis to Decipher the Range of Food Plants of the Invasive Spotted Lanternfly, Lycorma delicatula

Identifying Molecular-Based Trophic Interactions as a Resource for Advanced Integrated Pest Management

The challenge of biological control of Cosmopolites sordidus Germar (Col. Curculionidae): A review

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