It’s all about timing: Is the window of implantation different for day 5 and 6 blastocysts?

Kim, Helen H.; Matevossian, Karine

doi:10.1016/j.fertnstert.2020.04.031

Cited by 5 publications

(4 citation statements)

References 4 publications

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“…indicated that irrespective of the number of blastocysts formed, pregnancy rates were similar. The study by Roelens et al [ 25 ] suggested that some patients didn’t conceive after embryo transfer (ET) due to suboptimal timing rather than a pathologic issue. Meanwhile, one additional day of P treatment led to an increase in the live birth rate for the slower developing blastocyst embryos [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

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Competent blastocyst and receptivity endometrium improved clinical pregnancy in fresh embryo transfer cycles: a retrospective cohort study

Wang,

Qiu,

Jiang

et al. 2024

BMC Pregnancy Childbirth

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Background Embryo quality is usually regarded as a key predictor of successful implantation and clinical pregnancy potential. The identification of embryos that have the capacity to implant and result in a healthy pregnancy is a crucial part of in vitro fertilization (IVF). Usually, morphologically high-quality embryos are chosen for embryo transfer in IVF treatment. The aim of this study was to assess the association between the available blastocyst formation rate and the clinical pregnancy outcome following the first fresh embryo transfer cycle and provide systematic individual treatment to adjust endometrial receptivity for the next transfer cycle. Methods This retrospective, single-center study included 512 fresh embryo transfers conducted between 11/2019 and 08/2021, which consisted of 385 cleavage-stage (Day 3) and 127 blastocyst-stage (Day 5) embryo transfers. The two groups were divided into a clinical pregnancy group and a nonclinical pregnancy group for comparison. The association between the available blastocyst formation rate and the clinical pregnancy rate in the Day 3 and Day 5 transfer groups were considered. Results In the Day 3 group, there were 275 clinical pregnancies, and the clinical pregnancy rate was 71.43%. Although the two pronuclei (2PN) oocyte rate and available embryo rate at Day 3 were significantly higher in the clinical pregnancy group than the nonclinical pregnancy group (P < 0.05), the blastocyst formation rate and the available blastocyst formation rate were not significantly different between the clinical pregnancy group and the nonclinical pregnancy group (P > 0.05). In the Day 5 group, there were 81 clinical pregnancies, and the clinical pregnancy rate was 63.78%. No baseline characteristics showed any obvious differences between the clinical pregnancy group and nonclinical pregnancy group (P > 0.05). The blastocyst formation rate in the nonclinical pregnancy group was higher than that in the clinical pregnancy group, but the difference was not statistically significant (81.06% vs. 77.03%, P = 0.083). Interestingly, the available blastocyst formation rate and the Day 5 available blastocyst formation rate were significantly higher in the nonclinical pregnancy group than the clinical pregnancy group (66.19% vs. 60.79%, P = 0.014; 54.58% vs. 46.98%, P = 0.007). Conclusions In fresh cycles, the available blastocyst formation rate was not associated with the clinical pregnancy outcome for Day 3 embryo transfers, and the available blastocyst formation rate was not positively correlated with the clinical pregnancy outcome for Day 5 embryo transfers.

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Section: Discussionmentioning

confidence: 99%

“…2 ). However, embryos with low-speed growth rate are intrinsically different and require a longer duration of progesterone exposure for optimal synchronization with the endometrium [ 25 ]. We postulated that in our study, well-developed blastocysts adhered before or after the WOI and then missed the WOI (Fig.…”

Section: Discussionmentioning

confidence: 99%

Competent blastocyst and receptivity endometrium improved clinical pregnancy in fresh embryo transfer cycles: a retrospective cohort study

Wang,

Qiu,

Jiang

et al. 2024

BMC Pregnancy Childbirth

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“…Multiple reasons may lead to RIF, such as immune microenvironment homeostasis [4,5], embryo genetic abnormalities, endometrium receptivity [6,7], and expression of critical genes in the endometrium during the implantation window [8,9]. Embryo implantation is an important step of pregnancy establishment and occurs in a restricted period, termed the "window of implantation" [10]. During implantation, the embryo and the uterus go through synchronous development and bidirectional crosstalk, eventually establishing structural linkage and achieving material exchange [11,12].…”

Section: Introductionmentioning

confidence: 99%

Identification of target genes in recurrent implantation failure women associated with maternal-fetal immune

Fan

Liu

Zhang

et al. 2023

Preprint

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Background Recurrent implantation failure (RIF) is one of the main reasons for the unsatisfactory pregnancy rate of in vitro fertilization-embryo transfer, however the underlying mechanism is still obscure. Methods An online tool (Gene Expression Omnibus 2R) was used to identify gene expression differences in RIF women and fertility controls endometrium. Publicly available databases including GeneMANIA, Predicting Associated Transcription factors from Annotated Affinities, JASPAR and Protein Analysis Through Evolutionary Relationships were accessed to perform an integrated bioinformatic analysis. RIF and fertility controls patients (n = 9, respectively) were recruited from January to June 2022 at Affiliated Hospital of Changchun University of Traditional Chinese Medicine. Levels of target genes in the downstream of predicted signal pathway were measured by qPCR. Results Twenty-one different expression genes (DEGs), C11ORF65, DAPK2, KLK3, PFKFB2, SLC17A4, SLC7A1, CDC7, CDH13, DHRS3, DZIP1, GUCY1B3, KIAA0040, KIR2DL1, MAP3K5, NFKBIZ, SESTD1, TBXAS1, TIAM1, TIMP3, TNFAIP2 and TTC39C were found co-differential expression in GSE92324 and GSE103465 series, 93.19% displayed similar co-expression characteristics. In addition, the transcription factors (TFs) associated with key DEGs were recogonized, including RSF, FOR1, C/EBPALPHA, HNF-1ALPHA, AR, DP1, E2F1 and LEF1.The TFs associated diseases were focused on chromosome breakage, female infertility, embryo loss, and thrombosis, which acted a crucial role in RIF. Biological process analysis discovered that enrichment of these TFs correlated with IL-18 signal pathway, steroid hormone mediated signal pathway, DNA transcription, reproductive process and embryonic organ development. IL-18 signal pathway associated pro-immune molecules were upregulated in endometrium of RIF women than control. Conclusion The current research identified target genes and downstream signaling pathways at the maternal-fetal interface by bioinformatics-based analyses. It may provide a clue for prognosis judgement and target therapies in RIF women.

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“…The duration of progesterone therapy is critical for establishing the WOI. However, this optimal duration of progesterone administration before FET has not been established (14). There is an assumption that after estrogen priming, the number of days for exposure to progesterone will affect an appropriate endometrial lining to support the implantation of the blastocyst.…”

Section: Introductionmentioning

confidence: 99%

The exact synchronization timing between the cleavage embryo stage and duration of progesterone therapy-improved pregnancy rates in frozen embryo transfer cycles: A cross-sectional study

Omidi

Halvaei

Akyash

et al. 2021

IJRM

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Background: Synchronization between the embryonic stage and the uterine endometrial lining is important in the outcomes of the vitrified-warmed embryo transfer (ET) cycles. Objective: The aim was to investigate the effect of the exact synchronization between the cleavage stage of embryos and the duration of progesterone administration on the improvement of clinical outcomes in frozen embryo transfer (FET) cycles. Materials and Methods: 312 FET cycles were categorized into two groups: (A) day- 3 ET after three days of progesterone administration (n = 177) and (B) day-2 or -4 ET after three days of progesterone administration (n = 135). Group B was further divided into two subgroups: B1: day-2 ET cycles, that the stage of embryos were less than the administrated progesterone and B2: day-4 ET cycles, that the stage of embryos were more than the administrated progesterone. The clinical outcome measures were compared between the groups. Results: The pregnancy outcomes between groups A and B showed a significant differences in the chemical (40.1% vs 27.4%; p = 0.010) and clinical pregnancies (32.8% vs 22.2%; p = 0.040), respectively. The rate of miscarriage tended to be higher and live birth rate tended to be lower in group B than in group A. Also, significantly higher rates were noted in chemical pregnancy, clinical pregnancy, and live birth in group A when compared with subgroup B2. Conclusion: Higher rates of pregnancy and live birth were achieved in day-3 ET after three days of progesterone administration in FET cycles. Key words: Endometrium, Embryo transfer, Pregnancy, Live birth, Progesterone.

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It’s all about timing: Is the window of implantation different for day 5 and 6 blastocysts?

Cited by 5 publications

References 4 publications

Competent blastocyst and receptivity endometrium improved clinical pregnancy in fresh embryo transfer cycles: a retrospective cohort study

Competent blastocyst and receptivity endometrium improved clinical pregnancy in fresh embryo transfer cycles: a retrospective cohort study

Identification of target genes in recurrent implantation failure women associated with maternal-fetal immune

The exact synchronization timing between the cleavage embryo stage and duration of progesterone therapy-improved pregnancy rates in frozen embryo transfer cycles: A cross-sectional study

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