It is worth checking old data – validation of Asplenium onopteris L. presence in the most northeastern sites in Europe (Sudetes, SW Poland)

Abstract

In Poland, isolated serpentine rocks are exclusive habitats of some Asplenium species, reaching here their north or northeastern border range. One of them was Asplenium onopteris, a diploid European species native to Mediterranean and Atlantic areas. Since the nineteenth century, Polish out-of-range sites of A. onopteris have been quoted in literature without critical verification. Thus, to verify occurrence of this species in Poland, we analyzed the nuclear DNA content and micromorphological features as well as critically reviewed the literature data. We proved that all individuals from Polish populations resembling A. onopteris were tetraploids and should be classified as A. adiantum-nigrum. In addition, we validated a taxon silesiacum reported as co-occurring with A. onopteris. The proposed diagnostic features are insufficient to indisputably delimit this taxon, and distinguishing it as a separate unit is not justified. Analyses of the DNA content revealed also the presence of a triploid A. ×centovallense, a new hybrid for Polish flora.

Micromorphology of fern spores as a tool in taxonomy of East-Central European species from the family Aspleniaceae (Polypodiopsida)

“…e European flora contains 31 species of the family Aspleniaceae (Valentine & Moore, 2007), while in East-Central Europe, it is represented by ca. 25 taxa, including about a dozen hybrids (e.g., Jäger & Werner, 2005;Mirek et al, 2020;Reichstein, 1984;Szczęśniak et al, 2017). Many species are also subdivided into taxa of lower ranks (e.g., Danihelka et al, 2012;Didukh et al, 2000;Ekrt & Štech, 2008;Hassler, 2023; https://floraweb.de/; Jäger & Werner, 2005;Mosyakin & Fedoronchuk, 1999).…”

Morphology and genome size of Epipactis helleborine (L.) Crantz (Orchidaceae) growing in anthropogenic and natural habitats

“…Therefore, flow cytometry (FCM) is a more convenient alternative for establishing the ploidy/genome size of the Orchidaceae species. The genome size is, next to morphological descriptions, and molecular methods, good taxonomic marker useful for identifying many problematic taxa and phylogenetic reconstruction of closely related species (Ávila Diaz & Oyama, 2007; Fajardo, De Almeida Vieira & Molina, 2014; Rewers & Jedrzejczyk, 2016; Ståhlberg & Hedrén, 2008; Soliva, Kocyanb & Widmera, 2008; Szczęśniak, Gola & Jedrzejczyk, 2017; Wang et al, 2016). In the literature, we can find results, which show correlation between 2C DNA amount and morphological, ecological parameters of many groups of plants (Ahmadian et al, 2017; Knight, Molinari & Petrov, 2005; Knight & Ackerly, 2002).…”