It Is Time to Move: Role of Lateral Diffusion in AMPA Receptor Trafficking: Figure 1.

Yang, Guang; Huang, Alan Shuai; Zhu, Shanshan; Xiong, Wei

doi:10.1523/jneurosci.3018-06.2006

Cited by 7 publications

(4 citation statements)

References 8 publications

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“…We found that for our model, the spine neck radius affects the peak calcium and spatial gradients with a higher peak and larger gradient for a thinner neck, but has very limited effects on the accumulated calcium readout. The effect of the spine neck remains of interest however due to its documented ability to affect the diffusion of both cytoplasmic (Adrian et al, 2014) and membrane-bound proteins (Ashby et al, 2006;Yang et al, 2006;Ewers et al, 2014;Adrian et al, 2017) and its electrical resistance (Segev and Rall, 1998;Yuste, 2013). See Ohadi and Rangamani (2019) Preprint for spine neck variations at a longer timescale and Lee et al (2019) Preprint for realistic spine necks.…”

Section: Spine Neck Radius Has Limited Effects On Accumulated Calcium Readoutmentioning

confidence: 99%

Dendritic spine geometry and spine apparatus organization govern the spatiotemporal dynamics of calcium

Bell

Bartol

Sejnowski

et al. 2019

Journal of General Physiology

147

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Dendritic spines are small subcompartments that protrude from the dendrites of neurons and are important for signaling activity and synaptic communication. These subcompartments have been characterized to have different shapes. While it is known that these shapes are associated with spine function, the specific nature of these shape–function relationships is not well understood. In this work, we systematically investigated the relationship between the shape and size of both the spine head and spine apparatus, a specialized endoplasmic reticulum compartment within the spine head, in modulating rapid calcium dynamics using mathematical modeling. We developed a spatial multicompartment reaction–diffusion model of calcium dynamics in three dimensions with various flux sources, including N-methyl-D-aspartate receptors (NMDARs), voltage-sensitive calcium channels (VSCCs), and different ion pumps on the plasma membrane. Using this model, we make several important predictions. First, the volume to surface area ratio of the spine regulates calcium dynamics. Second, membrane fluxes impact calcium dynamics temporally and spatially in a nonlinear fashion. Finally, the spine apparatus can act as a physical buffer for calcium by acting as a sink and rescaling the calcium concentration. These predictions set the stage for future experimental investigations of calcium dynamics in dendritic spines.

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Section: Spine Neck Radius Has Limited Effects On Accumulated Calcium Readoutmentioning

confidence: 99%

Dendritic spine geometry and spine apparatus organization govern the spatiotemporal dynamics of calcium

Bell

Bartol

Sejnowski

et al. 2019

Journal of General Physiology

147

View full text Add to dashboard Cite

show abstract

“…Membrane receptors, including glutamate receptors ( Yang et al, 2006 ), have been demonstrated to flow from membrane protrusions towards the centre of the cell. This motion can be promoted by directed movement of the actin cytoskeleton ( Hanley, 2014 ; Yu et al, 2010 ) and its efficiency can be governed by the activation of the pathway ( Hartman et al, 2009 ).…”

Section: Discussionmentioning

confidence: 99%

Wnt- and glutamate-receptors orchestrate stem cell dynamics and asymmetric cell division

Junyent

Reeves

Szczerkowski

et al. 2021

eLife

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The Wnt-pathway is part of a signalling network that regulates many aspects of cell biology. Recently we discovered crosstalk between AMPA/Kainate-type ionotropic glutamate receptors (iGluRs) and the Wnt-pathway during the initial Wnt3a-interaction at the cytonemes of mouse embryonic stem cells (ESCs). Here, we demonstrate that this crosstalk persists throughout the Wnt3a-response in ESCs. Both AMPA- and Kainate-receptors regulate early Wnt3a-recruitment, dynamics on the cell membrane, and orientation of the spindle towards a Wnt3a-source at mitosis. AMPA-receptors specifically are required for segregating cell fate components during Wnt3a-mediated asymmetric cell division (ACD). Using Wnt-pathway component knockout lines, we determine that Wnt co-receptor Lrp6 has particular functionality over Lrp5 in cytoneme formation, and in facilitating ACD. Both Lrp5 and 6, alongside pathway effector β-catenin act in concert to mediate the positioning of the dynamic interaction with, and spindle orientation to, a localized Wnt3a-source. Wnt-iGluR crosstalk may prove pervasive throughout embryonic and adult stem cell signalling.

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“…Here, the spatial stabilization and colocalization of upstream and downstream pathway components at a ‘signalling hub’ may be critical for efficient signal transmission. Membrane receptors, including glutamate receptors (Yang et al, 2006), have been demonstrated to flow from membrane protrusion towards the centre of the cell. This motion can be promoted by directed movement of the actin cytoskeleton (Hanley, 2014; Yu et al, 2010) and its efficiency can be governed by the activation of the pathway (Hartman et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Wnt- and Glutamate-receptors orchestrate stem cell dynamics and asymmetric cell division

Junyent

Reeves

Szczerkowski

et al. 2020

Preprint

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13Wnt signalling regulates many aspects of cell biology. Wnt-pathway activation and its 14 downstream effects have been extensively studied, but the dynamic analysis of Wnt-ligands 15 on mammalian cellular membranes is obstructed by difficulties of visualization. We overcome 16 this using microbead-tethered Wnts presented to single embryonic stem cells, which undergo 17Wnt-mediated asymmetric cell division (ACD). Through live imaging and genetic editing, we 18show that knockout of Wnt co-receptor Lrp5 promotes cytoneme formation and Wnt-19 recruitment, which requires Lrp6 and β-catenin. Lrp5 facilitates ligand-retention at the 20 membrane, and alongside Lrp6 mediates Wnt-ligand stabilization and positioning. β-catenin 21 or Wnt co-receptor knockout causes misorientation at mitosis, and all but Lrp5 are required 22for Wnt-orientated ACD. Surprisingly, ionotropic glutamate receptor (iGluR) activity enables 23 initial Wnt-recruitment, positioning, and ultimately oriented ACD. Uniquely, we have 24 scrutinized the early Wnt ligand-membrane interaction, linking roles of Wnt-pathway 25 components and crosstalk with iGluRs in guiding cell fate determination by oriented ACD. 26 27

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It Is Time to Move: Role of Lateral Diffusion in AMPA Receptor Trafficking: Figure 1.

Cited by 7 publications

References 8 publications

Dendritic spine geometry and spine apparatus organization govern the spatiotemporal dynamics of calcium

Dendritic spine geometry and spine apparatus organization govern the spatiotemporal dynamics of calcium

Wnt- and glutamate-receptors orchestrate stem cell dynamics and asymmetric cell division

Wnt- and Glutamate-receptors orchestrate stem cell dynamics and asymmetric cell division

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