It Is Complicated: Curvature, Diffusion, and Lipid Sorting within the Two Membranes of <i>Escherichia coli</i>

Hsu, Pin-Chia; Samsudin, Firdaus; Shearer, Jonathan; Khalid, Syma

doi:10.1021/acs.jpclett.7b02432

Cited by 55 publications

(59 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To what extent do these simulations inform our understanding of the dynamic organisation of bacterial OMPs? Much recent work has focussed on modelling the interactions of LPS in such membranes 44 – 46 . LPS was not included in our simulations, as we wished to mimic the simpler in vitro system discussed above.…”

Section: Resultsmentioning

confidence: 99%

How nanoscale protein interactions determine the mesoscale dynamic organisation of bacterial outer membrane proteins

Chavent¹,

Duncan²,

Rassam³

et al. 2018

Nat Commun

View full text Add to dashboard Cite

The spatiotemporal organisation of membranes is often characterised by the formation of large protein clusters. In Escherichia coli, outer membrane protein (OMP) clustering leads to OMP islands, the formation of which underpins OMP turnover and drives organisation across the cell envelope. Modelling how OMP islands form in order to understand their origin and outer membrane behaviour has been confounded by the inherent difficulties of simulating large numbers of OMPs over meaningful timescales. Here, we overcome these problems by training a mesoscale model incorporating thousands of OMPs on coarse-grained molecular dynamics simulations. We achieve simulations over timescales that allow direct comparison to experimental data of OMP behaviour. We show that specific interaction surfaces between OMPs are key to the formation of OMP clusters, that OMP clusters present a mesh of moving barriers that confine newly inserted proteins within islands, and that mesoscale simulations recapitulate the restricted diffusion characteristics of OMPs.

show abstract

Section: Resultsmentioning

confidence: 99%

How nanoscale protein interactions determine the mesoscale dynamic organisation of bacterial outer membrane proteins

Chavent¹,

Duncan²,

Rassam³

et al. 2018

Nat Commun

View full text Add to dashboard Cite

show abstract

“…1A and D), a small population of this complex had been observed previously from recombinant preparations (17). Since a domain swapped dimer was observed by x-ray analysis of Bam E alone (18), and NMR solution studies were consistent with a population of BamE dimers (19), we docked into Bam ABCD a BamE dimer and used MD simulation to test its stability (20). The complex remained stable over 5 μs, following heating to 323 K, consistent with its viability in the E. coli outer membrane lipid environment.…”

mentioning

confidence: 74%

Faculty Opinions recommendation of Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry.

2019

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature

View full text Add to dashboard Cite

show abstract

“…Complex membrane protein environments can be treated with fewer limitations than at the all-atom level. been simulated, revealing that both membranes curve in a manner dependent on the size of the embedded proteins [223] ( Figure 12D). Similar approaches enabled the simulation of enveloped viruses whose shells encompass complex compositions [224][225][226].…”

Section: Simulating Biological Membrane Dynamics At Multiple Scalesmentioning

confidence: 99%

Visualizing Biological Membrane Organization and Dynamics

Baaden¹

2019

Preprint

View full text Add to dashboard Cite

Biological membranes are fascinating. Santiago Ramón y Cajal, who received the Nobel prize in 1906 together with Camillo Golgi for their work on the nervous system, wrote “[..]in the study of this membrane[..] I felt more profoundly than in any other subject of study the shuddering sensation of the unfathomable mystery of life”[1]. The visualization and conceptualization of these biological objects have profoundly shaped many aspects of modern biology, drawing inspiration from experiments, computer simulations, as well as from the imagination of scientists and artists. The aim of this review is to provide a fresh look on current ideas of biological membrane organization and dynamics by discussing selected examples across fields [1] The full quotation is “I must not conceal the fact that in the study of this membrane I for the first time felt my faith in Darwinism (hypothesis of natural selection) weakened, being amazed and confounded by the supreme constructive ingenuity revealed not only in the retina and in the dioptric apparatus of the vertebrates but even in the meanest insect eye. There, in fine, I felt more profoundly than in any other subject of study the shuddering sensation of the unfathomable mystery of life.” from the autobiography Recollections of My Life.

show abstract

It Is Complicated: Curvature, Diffusion, and Lipid Sorting within the Two Membranes of Escherichia coli

Cited by 55 publications

References 38 publications

How nanoscale protein interactions determine the mesoscale dynamic organisation of bacterial outer membrane proteins

How nanoscale protein interactions determine the mesoscale dynamic organisation of bacterial outer membrane proteins

Faculty Opinions recommendation of Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry.

Visualizing Biological Membrane Organization and Dynamics

Contact Info

Product

Resources

About