ISWI complexes in Saccharomyces cerevisiae

Mellor, Jane; Morillon, Antonin

doi:10.1016/j.bbaexp.2003.10.014

Cited by 87 publications

(81 citation statements)

References 79 publications

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“…In S.cerevisiae there are two ISWI genes -ISW1 and ISW2 (reviewed in [98]) which were identified based on their extensive homology with dISWI [75]. Isw1p forms two distinct complexes inside the cell -ISW1a (contains Isw1p, Ioc3p) and ISW1b (Isw1p, Ioc2p and Ioc4p) [99].…”

Section: Iswi Familymentioning

confidence: 99%

Mechanisms of ATP dependent chromatin remodeling

Gangaraju

Bartholomew

2007

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

128

159

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The inter-relationship between DNA repair and ATP dependent chromatin remodeling has begun to become very apparent with recent discoveries. ATP dependent remodeling complexes mobilize nucleosomes along DNA, promote the exchange of histones, or completely displace nucleosomes from DNA. These remodeling complexes are often categorized based on the domain organization of their catalytic subunit. The biochemical properties and structural information of several of these remodeling complexes are reviewed. The different models for how these complexes are able to mobilize nucleosomes and alter nucleosome structure are presented incorporating several recent findings. Finally the role of histone tails and their respective modifications in ATP-dependent remodeling are discussed.

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Section: Iswi Familymentioning

confidence: 99%

Mechanisms of ATP dependent chromatin remodeling

Gangaraju

Bartholomew

2007

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

128

159

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“…3A), we tested the possibility that a functional relationship exists between SWI/SNF and ISWI-like remodeling enzymes in transcriptional memory at GAL1. Budding yeast contain two distinct ISWIlike enzymes, Isw1 and Isw2 (Vary et al 2003;Mellor and Morillon 2004), and each functions as the catalytic subunit of distinct multisubunit remodeling complexes. We created isogenic isw1⌬, isw2⌬, isw1⌬ swi2⌬, and isw2⌬ swi2⌬ strains and monitored GAL1 reinduction kinetics.…”

Section: Loss Of Iswi-like Enzymes Restores Memory At Gal1 In the Absmentioning

confidence: 99%

SWI/SNF is required for transcriptional memory at the yeast GAL gene cluster

Kundu

Horn²,

Peterson³

2007

Genes Dev.

145

170

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Post-translational modification of nucleosomal histones has been suggested to contribute to epigenetic transcriptional memory. We describe a case of transcriptional memory in yeast where the rate of transcriptional induction of GAL1 is regulated by the prior expression state. This epigenetic state is inherited by daughter cells, but does not require the histone acetyltransferase, Gcn5p, the histone ubiquitinylating enzyme, Rad6p, or the histone methylases, Dot1p, Set1p, or Set2p. In contrast, we show that the ATP-dependent chromatin remodeling enzyme, SWI/SNF, is essential for transcriptional memory at GAL1. Genetic studies indicate that SWI/SNF controls transcriptional memory by antagonizing ISWI-like chromatin remodeling enzymes.[Keywords: Transcription; GAL1; SWI/SNF; chromatin, epigenetics, ISWI] Supplemental material is availabe at http://www.genesdev.org.

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“…They also include the highly conserved and mostly essential elongation factors, the Spt4/Spt5 complex, Spt6, and the Spt16/Pob3 complex. Additionally, the Paf1 complex (comprising Paf1, Ctr9, Rtf1, Leo1, and Cdc73), Isw1, Iws1, the Set1 complex, Set2, and Chd1 have also been implicated as part of the pol II elongation complex (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16).…”

mentioning

confidence: 99%

Interaction between Transcription Elongation Factors and mRNA 3′-End Formation at the Saccharomyces cerevisiae GAL10-GAL7 Locus

Kaplan

Holland

Winston

2005

Journal of Biological Chemistry

112

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Spt6 is a conserved transcription factor that associates with RNA polymerase II (pol II) during elongation. Spt6 is essential for viability in Saccharomyces cerevisiae and regulates chromatin structure during pol II transcription. Here we present evidence that mutations that impair Spt6, a second elongation factor, Spt4, and pol II can affect 3-end formation at GAL10. Additional analysis suggests that Spt6 is required for cotranscriptional association of the factor Ctr9, a member of the Paf1 complex, with GAL10 and GAL7, and that Ctr9 association with chromatin 3 of GAL10 is regulated by the GAL10 polyadenylation signal. Overall, these results provide new evidence for a connection between the transcription elongation factor Spt6 and 3-end formation in vivo.Many factors are believed to contribute to pol II 1 transcription elongation and to mRNA processing, based on either physical association with pol II, cotranscriptional association with transcribed DNA, or association with the nascent RNA (1-4). In Saccharomyces cerevisiae, these include factors involved in mRNA capping, splicing, termination, and export. They also include the highly conserved and mostly essential elongation factors, the Spt4/Spt5 complex, Spt6, and the Spt16/Pob3 complex. Additionally, the Paf1 complex (comprising Paf1, Ctr9, Rtf1, Leo1, and Cdc73), Isw1, Iws1, the Set1 complex, Set2, and Chd1 have also been implicated as part of the pol II elongation complex (5-16).Previous work has shown that Spt6 is broadly utilized in pol II transcription (17,18), and data from several laboratories implicates Spt6 as a pol II elongation factor (16, 18 -20). Spt6 has been shown to interact with histones and is involved in the organization of chromatin structure over transcribed regions (21-23). Spt6 has also been implicated in RNA processing, because Drosophila Spt6 is associated with the nuclear exosome (24). Additional roles for Spt6 in the transcription cycle probably also exist.The Paf1 complex is a multisubunit complex that associates with pol II and localizes to transcribed regions (14,16,(25)(26)(27)(28)(29). Although all of the roles of the Paf1 complex remain to be elucidated, recently, some complex members have been shown to be required for cotranscriptional ubiquitylation of histone H2B at position 123 (H2B Lys-123) and methylation of histone H3 lysines at positions 4 (Lys 4 ), 36 (Lys 36 ), and 79 (Lys 79 ) (30 -34). Thus, the Paf1 complex appears to coordinate histone modifications with transcription. Mutations in genes encoding Paf1 complex members also exhibit a wide spectrum of genetic interactions with mutations in elongation factor genes such as SPT4, SPT5, SPT16, and POB3 (14,35,36).As pol II goes through the cycle of transcription initiation, elongation, and termination, it is presented with different tasks. Many of these tasks are coordinated through the phosphorylation of the largest subunit of pol II on its conserved C-terminal domain, which in yeast consists of 26 repeats of consensus Tyr 1 -Ser 2 -Pro 3 -Thr 4 -Ser 5 -Pro 6 -Ser 7 ...

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ISWI complexes in Saccharomyces cerevisiae

Cited by 87 publications

References 79 publications

Mechanisms of ATP dependent chromatin remodeling

Mechanisms of ATP dependent chromatin remodeling

SWI/SNF is required for transcriptional memory at the yeast GAL gene cluster

Interaction between Transcription Elongation Factors and mRNA 3′-End Formation at the Saccharomyces cerevisiae GAL10-GAL7 Locus

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