ISTA13—International interlaboratory comparative evaluation of microbial assay for risk assessment (MARA)

Wadhia, K.

doi:10.1002/tox.20419

Cited by 11 publications

(7 citation statements)

References 12 publications

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“…The MARA (microbial assay for risk assessment) was carried out on 10 prokaryotic microbial species: Microbacterium sp., Brevundimonas diminuta , Citrobacter freundii , Comamonas testosteroni , Enterococcus casseliflavus , Delftia acidovorans , Kurthia gibsonii , Staphylococcus warnerii , Pseudomonas aurantiaca , Serratia rubidaea , and one eukaryote, Pichia anomalia , in order to measure toxic effects of chemicals contained in the unamended and amended soil samples. The growth of the organisms exposed to the tested samples was determined with the reduction of tetrazolium red (TZR). , A scanned image of the microplate obtained using a flatbed scanner was analyzed using purpose-built software. Six replications for each treatment and the unamended soil were analyzed.…”

Section: Materials and Methodsmentioning

confidence: 99%

Short-Term Effect of the Soil Amendments Activated Carbon, Biochar, and Ferric Oxyhydroxide on Bacteria and Invertebrates

Hale¹,

Jensen²,

Jakob³

et al. 2013

Environ. Sci. Technol.

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The aim of the present study was to evaluate the secondary ecotoxicological effects of soil amendment materials that can be added to contaminated soils in order to sequester harmful pollutants. To this end, a nonpolluted agricultural soil was amended with 0.5, 2, and 5% of the following four amendments: powder activated carbon (PAC), granular activated carbon, corn stover biochar, and ferric oxyhydroxide powder, which have previously been proven to sequester pollutants in soil. The resulting immediate effects (i.e., without aging the mixtures before carrying out tests) on the springtail Folsomia candida, the earthworm species Aporectodea caliginosa and Eisenia fetida, the marine bacteria Vibrio fischeri, a suite of ten prokaryotic species, and a eukaryote (the yeast species Pichia anomalia) were investigated. Reproduction of F. candida was significantly increased compared to the unamended soil when 2% biochar was added to it. None of the treatments caused a negative effect on reproduction. All amendments had a deleterious effect on the growth of A. caliginosa when compared to the unamended soil, except the 0.5% amendment of biochar. In avoidance tests, E. fetida preferred biochar compared to all other amendments including the unamended soil. All amendments reduced the inhibition of luminescence to V. fischeri, i.e., were beneficial for the bacteria, with PAC showing the greatest improvement. The effects of the amendments on the suite of prokaryotic species and the eukaryote were variable, but overall the 2% biochar dose provided the most frequent positive effect on growth. It is concluded that the four soil amendments had variable but never strongly deleterious effects on the bacteria and invertebrates studied here during the respective recommended experimental test periods.

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Section: Materials and Methodsmentioning

confidence: 99%

Short-Term Effect of the Soil Amendments Activated Carbon, Biochar, and Ferric Oxyhydroxide on Bacteria and Invertebrates

Hale¹,

Jensen²,

Jakob³

et al. 2013

Environ. Sci. Technol.

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“…where c min is the lowest concentration in the gradient, P O is the pellet size in the control, d is the dilution factor and P tot is the sum of all pellet sizes across the concentration gradient (Bronowska et al 2013; Sieroslawska 2014; Wadhia 2008). …”

Section: Methodsmentioning

confidence: 99%

Toxicity and biodegradation of ibuprofen by Bacillus thuringiensis B1(2015b)

Marchlewicz

Guzik

Hupert-Kocurek

et al. 2017

Environ Sci Pollut Res

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In recent years, the increased intake of ibuprofen has resulted in the presence of the drug in the environment. This work presents results of a study on degradation of ibuprofen at 25 mg L−1 in the presence of glucose, as an additional carbon source by Bacillus thuringiensis B1(2015b). In the cometabolic system, the maximum specific growth rate of the bacterial strain was 0.07 ± 0.01 mg mL−1 h−1 and K sμ 0.27 ± 0.15 mg L−1. The maximum specific ibuprofen removal rate and the value of the half-saturation constant were q max = 0.24 ± 0.02 mg mL−1 h−1 and K s = 2.12 ± 0.56 mg L−1, respectively. It has been suggested that monooxygenase and catechol 1,2-dioxygenase are involved in ibuprofen degradation by B. thuringiensis B1(2015b). Toxicity studies showed that B. thuringiensis B1(2015b) is more resistant to ibuprofen than other tested organisms. The EC50 of ibuprofen on the B1 strain is 809.3 mg L−1, and it is 1.5 times higher than the value of the microbial toxic concentration (MTCavg). The obtained results indicate that B. thuringiensis B1(2015b) could be a useful tool in biodegradation/bioremediation processes.

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“…The MTC value was calculated according to the formula: (1) …where c min is the lowest concentration in the gradient, P o is pellet size in the control, d is dilution factor, and P tot is sum of all the pellets sizes across the concentration gradient [13][14][15].…”

Section: Toxicity Bioassaysmentioning

confidence: 99%