Issr analysis of some species of angustifoliate fescue

Bednarska, Iryna; Popov, V. N.; Dugar, Yu. N.; Akinina, G. E.; Dolgova, T. A.

doi:10.3103/s0095452714060024

Cited by 4 publications

(5 citation statements)

References 18 publications

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“…Furthermore they require only a small amount of DNA; this is why they are often employed in the genetic evaluation of different plant species. They can be used either in combination ( Nkongolo, Michael & Demers, 2005 ; Alam, Naik & Mishra, 2009 ; De Lima et al, 2011 ; Singh et al, 2011 ; Debajit et al, 2015 ) or independently of each other ( Al-Humaid & Motawei, 2004 ; Wang, 2010 ; Guo et al, 2009 ; Bednarskaya et al, 2014 ). In grasses, RAPD and ISSR markers are most commonly used to evaluate genetic variability within and between populations, and for molecular characterization and identification of different species and their hybrids, cultivars and genotypes ( Posselt et al, 2006 ; Pivorienė et al, 2008 ; Al-Humaid, Ibrahim & Motawei, 2011 ; Motawei & Al-Ghumaiz, 2012 ; Madesis et al, 2014 ; Yuan et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…The highest number of DNA fragments were obtained in the presence of these primers. In the case of ISSR markers, as in the studies of Bednarskaya et al (2014) , better results were obtained in the presence of primers with dinucleotide repeat sequences (AG) 2 ,(GA) 2 and (AC) 2 , anchored at the end of 3’. As suggested by Al-Humaid et al (2004) and Al-Humaid, Ibrahim & Motawei (2011) , dinucleotide and trinucleotide repeats are abundant in genomes of fodder grasses and thus might constitute potential markers for assessing their genetic diversity and identifying their fungal diseases using the ISSR method.…”

Section: Discussionmentioning

confidence: 99%

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Genetic diversity ofPoa pratensisL. depending on geographical origin and compared with genetic markers

Szenejko

Śmietana

Stępień

2016

PeerJ

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BackgroundPoa pratensis is one of the most common species of meadow grass in Europe. Most cultivars of the species found in Poland were originally derived from its ecotypes. We compared the effectiveness of the RAPD and ISSR methods in assessing the genetic diversity of the selected populations of P. pratensis. We examined whether these methods could be useful for detecting a possible link between the geographical origin of a given population and its assessed genetic variation.MethodsThe molecular markers RAPD and ISSR were used and their efficiency compared using, inter alia, statistical multivariate methods (UPGMA and PCA).ResultsThe low value of Dice’s coefficient (0.369) along with the significantly high percentage of polymorphic products indicates a substantial degree of genetic diversity among the studied populations. Our results found a correlation between the geographical origin of the studied populations and their genetic variations. For ISSR, which proved to be the more effective method in that respect, we selected primers with the greatest differentiating powers correlating to geographical origin.DiscussionThe populations evaluated in this study were characterized by a high genetic diversity. This seems to confirm the hypothesis that ecotypes of P. pratensis originating from different regions of Central Europe with different terrain structures and habitat conditions can be a source of great genetic variability.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genetic diversity ofPoa pratensisL. depending on geographical origin and compared with genetic markers

Szenejko

Śmietana

Stępień

2016

PeerJ

View full text Add to dashboard Cite

show abstract

“…Furthermore they require only a small amount of DNA; this is why they are often employed in the genetic evaluation of different plant species. They can be used either in combination (Nkongolo et al, 2005;Alam et al, 2009;de Lima et al, 2011;Singh et al, 2011;Debajit et al, 2015) or independently of each other (Al-Humaid & Wang, 2010;Guo et al, 2009;Bednarskaya et al 2014). In grasses, RAPD and ISSR markers are most commonly used to evaluate genetic variability within and between populations, and for molecular characterization and identification of different species and their hybrids, cultivars and genotypes (Posselt et al, 2006;Pivorienė et al, 2008;Al-Humaid et al, 2011;Motawei & Al-Ghumaiz, 2012;Madesis et al, 2014;Yuan et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…The highest number of DNA fragments were obtained in the presence of these primers. In the case of ISSR markers, as in the studies of Bednarskaya et al (2014), better results were obtained in the presence of primers with dinucleotide repeat sequences (AG) 2, (GA) 2 and (AC) 2, anchored at the end of 3'. As suggested by , dinucleotide and trinucleotide repeats are abundant in genomes of fodder grasses and thus might constitute potential markers for assessing their genetic diversity and identifying their fungal diseases using the ISSR method.…”

Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "Genetic diversity of Poa pratensis L. depending on geographical origin and compared with genetic markers (v0.1)"

2016

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Background. Poa pratensis is one of the most common species of meadow grass in Europe. Most cultivars of the species found in Poland were originally derived from its ecotypes. We compared the effectiveness of the RAPD and ISSR methods in assessing the genetic diversity of the selected populations of P. pratensis. We examined whether these methods could be useful for detecting a possible link between the geographical origin of a given population and its assessed genetic variation. Methods. The molecular markers RAPD and ISSR were used and their efficiency compared using, inter alia, statistical multivariate methods (UPGMA and PCA). Results. The low value of Dice's coefficient (0.369) along with the significantly high percentage of polymorphic products indicates a substantial degree of genetic diversity among the studied populations. Our results found a correlation between the geographical origin of the studied populations and their genetic variations. For ISSR, which proved to be the more effective method in that respect, we selected primers with the greatest differentiating powers correlating to geographical origin. Discussion. The populations evaluated in this study were characterized by a high genetic diversity. This seems to confirm the hypothesis that ecotypes of P. pratensis originating from different regions of Central Europe with different terrain structures and habitat conditions can be a source of great genetic variability.

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“…Según este índice los cebadores 841, 812, 810, 834 y BOR2 podrían ser usados en futuras caracterizaciones del germoplasma de cañihua. Terzopoulos et al, (2008) muestran en sus estudios que cada cebador amplifica diferente patrón obteniendo diferente número de bandas polimórficas; así por ejemplo, los cebadores 810 y 825 ambos con temperatura de anclaje (annealing) de 51 °C fueron seleccionados como polimórficos en el estudio de variación genética de especies de Angustifoliate (Bednarskaya et al 2014)…”

Section: Pic (íNdice De Contenido Polimórfico)unclassified

Caracterización molecular de la cañihua (chenopodium Pallidicaule aellen)

et al. 2022

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Se realizó una estandarización del método de extracción de ADN para cañihua (Chenopodium pallidicaule Aellen) y la caracterización molecular mediante marcadores moleculares ISSR (Inter Simple Sequence Repeat). Se utilizaron hojas de 40 accesiones de cañihua tomadas del banco de germoplasma del Programa de Cereales y Granos Nativos de la UNALM provenientes de dos provincias de Puno. Se realizó la extracción del ADN mediante el método CTAB. La agregación de cloroformo: alcohol isoamílico (24:1) y del etanol 96% permitieron la extracción de ADN de buena calidad. Obtenido el ADN, se realizó la amplificación PCR, PAGE y tinción con nitrato de plata. Se evaluó el porcentaje de loci polimórficos, contenido de información polimórfica y análisis de variancia molecular. Se seleccionaron 7 cebadores que produjeron 52 locus polimórficos de 144 marcadores ISSR, siendo los más informativos 841, 812, 810, 834 y BOR2. Una alta diferenciación genética entre poblaciones fue detectada basada en el AMOVA (Fst = 0.1544). Esto hace suponer que, la posición geográfica marca la diferenciación genética entre poblaciones de diferentes provincias.

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Issr analysis of some species of angustifoliate fescue

Cited by 4 publications

References 18 publications

Genetic diversity ofPoa pratensisL. depending on geographical origin and compared with genetic markers

Genetic diversity ofPoa pratensisL. depending on geographical origin and compared with genetic markers

Peer Review #1 of "Genetic diversity of Poa pratensis L. depending on geographical origin and compared with genetic markers (v0.1)"

Caracterización molecular de la cañihua (chenopodium Pallidicaule aellen)

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