Isozyme polymorphism of cellulases in <i>Aspergillus terreus</i>

Singh, Surinder; Brar, J. K.; Sandhu, D. K.; Kaur, Amrita

doi:10.1002/jobm.3620360412

Cited by 11 publications

(8 citation statements)

References 26 publications

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“…Similarly cellulases were produced from Aspergillus niger KK2 at 28°C [35]. Similarly, maximum cellulase production in Aspergillus terreus was obtained at a temperature of 28° C [36].…”

Section: Xylanase Assaymentioning

confidence: 91%

Effect of Various Physiological Parameters and Different Carbon Sources on Cellulase and Xylanase Induction by Different Strains of Trichoderma Species

Singh¹

2014

Enz Eng

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BGLs is subjected to produce inhibition and though it is sufficient to support growth on cellulosic material; it is often supplemented with Aspergillus BGLs for complete biomass saccharification at industrial level [10]. Nevertheless, it is the best cellulase available today. The major bottle neck against comprehensive application of cellulases in biofuel industry is the high cost of the enzyme production. Therefore, extensive research is needed to enhance the enzyme activity so that less enzyme is needed for the complete hydrolysis of biomass. Cellulose quality, temperature, carbon sources, incubation period and pH of the medium are important parameters for the optimized production of cellulase enzymes [11]. Considering the importance and application of cellulases, present study was aimed to isolate and screen the fungal isolates for the cellulolytic ability. Moreover, in the present study three different substrates such as wheat bran, birch wood xylan and cellulose powder were used and the effect of various parameters like pH and temperature were also studied on cellulase production. Materials and Methods Chemical reagents All chemicals and reagents used were of analytical grade. Different carbon sources such as birch wood xylan, Salicin, Carboxy-methyl

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“…Similarly cellulases were produced from Aspergillus niger KK2 at 28°C [35]. Similarly, maximum cellulase production in Aspergillus terreus was obtained at a temperature of 28° C [36].…”

Section: Xylanase Assaymentioning

confidence: 91%

Effect of Various Physiological Parameters and Different Carbon Sources on Cellulase and Xylanase Induction by Different Strains of Trichoderma Species

Singh¹

2014

Enz Eng

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“…All four classes of enzymes have been identified in aspergilli, although the number of isozymes produced by different species or even strains of the same species can differ. An analysis of the production of endoglucanases by 45 A. terreus isolates not only revealed different electrophoretic mobilities for the enzymes of the different isolates but also indicated the absence of endoglucanase I in a number of the isolates (341). Endoglucanases and ␤-glucosidases are also able to degrade the backbone of xyloglucan.…”

Section: Degradation Of Cellulose and The Xyloglucan Backbonementioning

confidence: 99%

AspergillusEnzymes Involved in Degradation of Plant Cell Wall Polysaccharides

Vries

Visser

2001

Microbiol Mol Biol Rev

877

585

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SUMMARY Degradation of plant cell wall polysaccharides is of major importance in the food and feed, beverage, textile, and paper and pulp industries, as well as in several other industrial production processes. Enzymatic degradation of these polymers has received attention for many years and is becoming a more and more attractive alternative to chemical and mechanical processes. Over the past 15 years, much progress has been made in elucidating the structural characteristics of these polysaccharides and in characterizing the enzymes involved in their degradation and the genes of biotechnologically relevant microorganisms encoding these enzymes. The members of the fungal genus Aspergillus are commonly used for the production of polysaccharide-degrading enzymes. This genus produces a wide spectrum of cell wall-degrading enzymes, allowing not only complete degradation of the polysaccharides but also tailored modifications by using specific enzymes purified from these fungi. This review summarizes our current knowledge of the cell wall polysaccharide-degrading enzymes from aspergilli and the genes by which they are encoded.

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“…Moreover, it was also noted that the addition of 0.5% glucose in the fermentation medium does not induce catabolic repression. Wheat bran, CMC and glucose were reported to be the inducer of cellulase (Dhillon, Chhibber, Saxena, Pajni, & Vadehra, 1985;Ryu & Mandels, 1980;Singh, Brar, Sandhu, & Kaur, 1996). It is assumed that CMC is less complex structure and therefore, easily assimilating of it by the microorganism (Wood & Bhat, 1988).…”

Section: 2mentioning

confidence: 99%

Hyper production of cellulose degrading endo (1,4) β-d-glucanase from Bacillus licheniformis KIBGE-IB2

Karim

Nawaz

Aman

et al. 2015

Journal of Radiation Research and Applied Sciences

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Cellulase hydrolyzes b (1,4) glycosidic linkages of cellulose polymer to soluble sugar. An extracellular enzyme production by Bacillus licheniformis KIBGE-IB2 (GenBank accession No. GU216259) was studied under various environmental conditions. Maximum enzyme production was measured in the liquid fermentation medium after 48 h, containing (gL À1), CMC, 5.0; peptone, 15.0; yeast extract, 15.0; CaCl 2 $2H 2 O, 0.001; FeSO 4 $7H 2 O, 0.001; K 2 HPO 4, 5.0 ; NaH 2 PO 4, 5.0 and MgSO 4 $7H 2 O, 1.0. The optimal pH and temperature for enzyme production was found to be 6.0 and 37 C, respectively. It was also found that beside soluble sugars, a significant amount of enzyme production was obtained when biomass (wheat bran and orange peel) were examined as a sole carbon source. The current findings indicate that endo (1,4) b-D-glucanase from B. licheniformis KIBGE-IB2 can be beneficial for commercial purpose.

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Isozyme polymorphism of cellulases in Aspergillus terreus

Cited by 11 publications

References 26 publications

Effect of Various Physiological Parameters and Different Carbon Sources on Cellulase and Xylanase Induction by Different Strains of Trichoderma Species

Effect of Various Physiological Parameters and Different Carbon Sources on Cellulase and Xylanase Induction by Different Strains of Trichoderma Species

AspergillusEnzymes Involved in Degradation of Plant Cell Wall Polysaccharides

Hyper production of cellulose degrading endo (1,4) β-d-glucanase from Bacillus licheniformis KIBGE-IB2

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