Isozyme Evidence for Natural Hybridization in Phlomis (Lamiaceae): Hybrid Origin of the RareP.×margaritae

Aparicio, Abelardo

doi:10.1006/anbo.1999.0987

Cited by 17 publications

(9 citation statements)

References 30 publications

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“…However, to our knowledge hybridization in the genus has only been unambiguously confirmed with the use of molecular markers for P. ×margaritae Aparicio & Silvestre (P. crinita subsp. malacitana × P. purpurea), an F 1 hybrid with only one known population in southern Spain (Aparicio & al., 2000). This might represent an example of hybridization between relatively distant lineages, which do not pose serious problems of identification.…”

Section: Introductionmentioning

confidence: 99%

Contrasting nuclear‐plastidial phylogenetic patterns in the recently diverged IberianPhlomis crinitaandP. lychnitislineages (Lamiaceae)

Albaladejo

Aguilar

Aparicio

et al. 2005

TAXON

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Systematic and evolutionary relationships within the diploid Western Mediterranean Phlomis crinita/lychnitis complex remain controversial apparently due to hybridization and introgression. This study examines patterns of sequence variation in the nuclear ribosomal DNA (ITS region) and three non‐coding plastid DNA regions (trnH‐psbA, trnT‐trnL and atpB‐rbcL) in this complex in an aim to clarify whether hybridization, introgression or lineage sorting resulting from recent diversification is mainly responsible for poorly understood relationships. Information recovered from nuclear and chloroplast markers was found to be strongly incongruent. Phylogenetic analysis of inferred nuclear ITS ribotypes is consistent with previous morphometric and taxonomic results in distinguishing two sister lineages, P. crinita s.l. and P. lychnitis. In addition, the high number of additive polymorphisms detected in ITS sequences suggests the sharing of ancestral variability and local patterns of gene flow within the complex. In contrast, the pattern of chloroplast haplotype variation is geographic rather than taxonomic, which might be caused by low mutation rates combined with frequent instances of interspecific hybridization. To integrate the apparently discordant plastid and nuclear evidence, we suggest that both lineage sorting and horizontal transfer have been involved. Even though pinpointing cases due to either phenomenon is difficult, the available data provide evidence of two sister lineages where hybridization events can be identified despite the inheritance of ancestral polymorphisms.

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Section: Introductionmentioning

confidence: 99%

Contrasting nuclear‐plastidial phylogenetic patterns in the recently diverged IberianPhlomis crinitaandP. lychnitislineages (Lamiaceae)

Albaladejo

Aguilar

Aparicio

et al. 2005

TAXON

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“…Irrespective of the taxonomic view, some of these presumably hybrid forms were found to be relatively fertile and diploid (2 n = 20) (Aparicio, 1997) and hybridization was confirmed for the sterile P. x margaritae Aparicio & Silvestre ( P. x composita nothom. malacitana x P. purpurea L.) using allozyme variation as genetic markers (Aparicio et al ., 2000).…”

Section: Introductionmentioning

confidence: 99%

Microsporogenesis and meiotic abnormalities in the hybrid complex of Phlomis composita (Lamiaceae)

Aparicio

Albaladejo

2003

Botanical Journal of the Linnean Society

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The extent of chromosome structural differences such as permanent translocations and paracentric inversions has been studied in the hybrid complex of Phlomis composita and its putative parental taxa P. lychnitis and P. crinita . They are detected as meiotic abnormalities during microsporogenesis. In total, 117 plants from 19 populations in Andalusia (southern Spain) and Levante (eastern Spain) were studied for ploidy level, chromosome clumping, multivalent or univalent formation, late bivalent disjunction with bridges, chromatin bridges and fragments, irregular polysporads and variable pollen size. All individuals had the diploid chromosome number 2 n = 20, with no significant difference in the frequencies of the above meiotic abnormalities either at taxon or population levels. However, very different frequencies were observed on the regional scale between populations in Andalusia and Levante. The results are discussed within the theoretical framework of homoploid hybrid or recombinational speciation.

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“…Hybrization between Phlomis species has been indicated by using morphological data (Albaladejo et al 2004), isozymes (Aparicio et al 2000) and DNA markers such as ITS (Albaladejo et al 2005) and RAPDs (Yuzbasioglu et al 2008). Albaladejo et al (2004) studied natural hybridization between P. lychnitis and P. crinita subsp.…”

Section: Resultsmentioning

confidence: 99%

Phylogenetic relationships among species of the subsection Dendrophlomis Bentham

Yüzbasioglu¹,

Dadandi²

2008

Electron. J. Biotechnol.

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Abbreviations: AMOVA: analysis of molecular variance ITS: internal transcribed spacer PCR: polymerase chain reaction RAPDs: randomly amplified polymorphic DNA UPGMA: unweighted pair-group method with arithmetic averages This study used randomly amplified polymorphic DNA markers to determine genetic relationships among species of the subsection Dendrophlomis. Twenty accessions of the eleven Phlomis taxa were evaluated to determine genetic variability using fourteen ten mer primers selected from a 125 random oligonucleotide set. These 14 selected primers generated 85 RAPD bands that ranged in size from 200 to 1200 base pairs. Of the total bands, 88% (75) were polymorphic among the samples. Genetic distances among accessions were computed to produce a dendrogram based on UPGMA. Genetic distances ranged from 0.133 (between P. amanica and P. monocephala) to 0.494 (between P. chimerae and P. lunariifolia). The UPGMA tree based on distances has two major groups. The first comprised 9 taxa that were clustered into two subgroups. The first subgroup consisted of P. viscosa, P. lycia, P. amanica and P. monocephala while the second comprised P. lunariifolia, P. bourgaei, P. longifolia var. longifolia, P. grandiflora var. grandiflora and P. grandiflora var. fimbrilligera. The second group comprised 2 species, P. leucophracta and P. chimerae. Species-specific bands were observed for P. lycia, P. leucophracta, P. lunariifolia, P. bourgaei, P. chimerae and P. longifolia var longifolia. *Corresponding authorThe genus Phlomis L. comprises over 100 species including herbs, shrubs and sub-shrubs of the family Lamiaceae (Albaladejo et al. 2005). The genus is divided into two main sections, Phlomis and Phlomoides (Moench, 1794). Both sections are spread from the Mediterranean region to central Asia and China; but while species of the section Phlomoides occur mostly in central Asia and China, species of the section Phlomis appear mainly in the Mediterranean region. Turkey and Iran were indicated as the main centers of diversification in the Mediterranean region for the section Phlomis (Hedge, 1986). In particular, southern and eastern parts of the former and north-western part of the latter were proposed as centers of origin of that section. Nevertheless, Turkey has twice the number of species (34) and also nearly twice a higher endemism rate (57%) of species belonging to section Phlomis compared to Iran, where the numbers are 18% and 33% respectively (Hedge, 1986).Measurement of genetic variation within and between plant species is important for several reasons including delimitation of species, conservation of endangered species and construction of phylogenetic relationships among species. Several kinds of methods were used to measure levels and patterns of genetic variation, which range from Yüzbaşıoğlu, E. and Dadandı, M.Y.2 morphological characterization to various DNA-based markers such as restriction fragment length polymorphism (RFLPs), randomly amplified polymorphic DNA (RAPDs), amplified fragment length polymorphism (AFLPs) ...

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Isozyme Evidence for Natural Hybridization in Phlomis (Lamiaceae): Hybrid Origin of the RareP.×margaritae

Cited by 17 publications

References 30 publications

Contrasting nuclear‐plastidial phylogenetic patterns in the recently diverged IberianPhlomis crinitaandP. lychnitislineages (Lamiaceae)

Contrasting nuclear‐plastidial phylogenetic patterns in the recently diverged IberianPhlomis crinitaandP. lychnitislineages (Lamiaceae)

Microsporogenesis and meiotic abnormalities in the hybrid complex of Phlomis composita (Lamiaceae)

Phylogenetic relationships among species of the subsection Dendrophlomis Bentham

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