Isozyme electrophoregrams of sixteen enzymes in five tissues of cassava (Manihot esculenta
Crantz) varieties

Ramírez, Hernando; Hussain, A.; Roca, William M.; Bushuk, W.

doi:10.1007/bf00730645

Cited by 30 publications

(15 citation statements)

References 11 publications

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“…Different authors have shown the interest of enzyme electrophoresis for the studies of cassava genetics, although some phenotypes were unstable depending on the origin of the extract and the buffers (Zoundjihekpon & Tour6, 1983;Ramirez et al, 1987). In our study, we were able to give a genetic interpretation for most of the phenotypes, after fixing standard conditions for extraction, migration and staining.…”

Section: Discussionmentioning

confidence: 52%

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Heredity of seventeen isozyme loci in cassava (Manihot esculenta Crantz)

Lefèvre

Charrier²

1992

Euphytica

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Starch gel electrophoresis was used to assess isozyme polymorphism in two Manihot species. Crude extracts were obtained from leaves and pollen. Ten enzymes were examined for their polymorphism in a germplasm collection of 365 cultivated plus 109 wild accessions, mainly from Africa. The inheritance of these enzymes was examined using 13 intra and interspecific progenies. Seventeen polymorphic loci were found for the ten enzyme systems, with 59 alleles. All the markers showed disomic heredity and three linkage groups were identified.

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Section: Discussionmentioning

confidence: 52%

“…The first attempts to use enzyme electrophoresis on cassava showed a high level of polymorphism within the species (Zoundjihekpon & Tour6, 1985;Hussain et al, 1987;Ramirez et al, 1987). We used starch gel electrophoresis as a cheap and simple technique to identify genetic markers from 10 enzyme systems.…”

Section: Introductionmentioning

confidence: 99%

Heredity of seventeen isozyme loci in cassava (Manihot esculenta Crantz)

Lefèvre

Charrier²

1992

Euphytica

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“…Agronomical and botanical descriptors (Pereira et al, 1992;Cury, 1993) and isoenzyme analysis (Ramirez et al, 1987;Léfèvre et al, 1993) have been developed to evaluate genetic diversity. However, a limited number of isoenzymatic systems (Pasteur et al, 1987) and botanical descriptors (Cury, 1993) are available; therefore, polymorphisms are restricted.…”

Section: Introductionmentioning

confidence: 99%

Diversity within American cassava germ plasm based on RAPD markers

Colombo

Second²,

Charrier³

2000

Genet. Mol. Biol.

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This work focuses on the genetic diversity of American cassava through RAPD molecular markers. The 126 genotypes studied were distributed on four geographical levels ranging from local to continental. Samples included ethnocultivars from the Santa Isabel community in the Brazilian Amazon, local cultivars collected in the State of São Paulo, native accessions from very diverse Brazilian regions, and representative accessions from the Centro Internacional de Agricultura Tropical (CIAT) core collection. Eighty-eight polymorphic bands were analyzed. Results revealed the weak genetic structure of the cassava analyzed. The pattern formed by the first two axes of the principal coordinates analysis (PCoA) revealed an overlapping of the São Paulo State genotype, the Brazilian group and the core collection accessions. The Santa Isabel ethnocultures formed a separate group. The weak genetic structure of cassava can be explained by the common practice of exchanging botanical material among small producers as well as by recombinations among genotypes. When the genotypes were analyzed using climatic data, the sample sites were found to be structured according to temperature and precipitation. RAPD markers proved very useful in the genetic diversity study, resulting in important implications for cassava germ plasm collections and genetic breeding.

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“…For the molecular characterization, we used seeds and seedlings of 443 individuals coming from six populations collected in 1987, 1998, Zarcero (48), Aserrí (84), Tarbaca (18), Jérico (64), and Quircot (119). Seed storage protein phaseolin and two enzymes (diaphorase and peroxydase) were analyzed following the protocol by Lareo et al (1993) and Ramírez et al (1987), respectively. The interpretation of the diaphorase pattern was done along Sprecher (1988), while we followed the procedure by Koenig & Gepts (1989) for peroxydase.…”

Section: Methodsmentioning

confidence: 99%