Isozyme Diversity in Wild Red Clover Populations from the Caucasus

Mosjidis, J. A.; Greene, Stephanie L.; Klingler, Kimberly A.; Afonin, A. N.

doi:10.2135/cropsci2004.1039

Cited by 8 publications

(11 citation statements)

References 2 publications

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“…Its value (H e = 0.31), indicating and average frequency of about 80% for the more common allele was comparable with those detected in red clover populations from Caucasus (Mosjidis et al 2004), north America (Hagen and Hamrick 1998), and the USDA-ARS collection (Yu et al 2001), while being lower than that reported through SSR markers for Swiss red clover populations (Kölliker et al 2006).…”

Section: Discussionsupporting

confidence: 63%

“…Our findings have implications on germplasm collection and conservation activities (including the establishment of core collections), highlighting the importance of the environment of evolution as the main determinant Table 1 Euphytica (2011) 179:393-404 401 of germplasm diversification for traits of major interest for breeders. The value of total genetic diversity estimated for the current germplasm through AFLP markers (H T = 0.43) was higher than those estimated through allozymes and isozyme in north American feral populations and a the USDA world collection (H T = 0.32, Hagen and Hamrick 1998; and H T = 0.36-0.39, Mosjidis and Klingler 2006), RAPD markers in accessions from South America and Switzerland (H T = 0.29; Ulloa et al 2003), and isozymes in American cultivars (H T = 0.29; Yu et al 2001) or 15 Caucasian natural populations (H T = 0.35; Mosjidis et al 2004). Besides high diversity, the Italian germplasm also showed limited estimated geneflow overall (averaging Nm = 0.65) and in distinct subregions relative to mean values of Caucasian (Nm ranging from 0.75 to 12.07; Mosjidis et al 2004) or north American (Nm = 3.36; Hagen and Hamrick 1998) populations.…”

Section: Discussionmentioning

confidence: 85%

“…The value of total genetic diversity estimated for the current germplasm through AFLP markers (H T = 0.43) was higher than those estimated through allozymes and isozyme in north American feral populations and a the USDA world collection (H T = 0.32, Hagen and Hamrick 1998; and H T = 0.36-0.39, Mosjidis and Klingler 2006), RAPD markers in accessions from South America and Switzerland (H T = 0.29; Ulloa et al 2003), and isozymes in American cultivars (H T = 0.29; Yu et al 2001) or 15 Caucasian natural populations (H T = 0.35; Mosjidis et al 2004). Besides high diversity, the Italian germplasm also showed limited estimated geneflow overall (averaging Nm = 0.65) and in distinct subregions relative to mean values of Caucasian (Nm ranging from 0.75 to 12.07; Mosjidis et al 2004) or north American (Nm = 3.36; Hagen and Hamrick 1998) populations. The limited geneflow concurs to explain the lack of relationship between geographic proximity and molecular similarity of the populations, suggesting that the among-population variation for marker alleles which originated from local selection or genetic shift was largely maintained at the population level.…”

Section: Discussionmentioning

confidence: 85%

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Characterizing the molecular and morphophysiological diversity of Italian red clover

et al. 2010

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Red clover (Trifolium pratense L.) is the third major forage species in Europe, but there is limited information on the biodiversity and the genetic structure of landraces and natural populations which evolved in this region. The objective of this study was producing such information for Italian germplasm on the ground of molecular and morphophysiological diversity. The study included 16 Italian natural populations from a wide range of environments, four landraces representing the four traditional commercial ecotypes, and two varieties. Eight morphophysiological traits were assessed in a replicated trial under field conditions, whereas two AFLPs primer combinations with 140 polymorphic markers were recorded on a random sample of 13 genotypes per population. Ordination and classification results based on morphophysiological data clearly kept track of the type of germplasm (i.e. landrace or natural population) and its geographic origin, unlike results based on molecular markers. Euclidean distances among populations based on morphophysiological traits were not correlated with Nei's genetic distances based on molecular markers according to Mantel's test. Geographical distances among landrace or natural population material was correlated with distances based on morphophysiological traits but not with those based on molecular markers. The average within-population variation estimated via molecular markers was about 2.6-fold higher than that among populations, preventing an acceptable discrimination among most populations. On average, natural populations tended to have within-population variation similar to varieties and somewhat lower than landraces. Our results have implications for collection, conservation, exploitation and registration in a sui generis system of red clover genetic resources.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 85%

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Characterizing the molecular and morphophysiological diversity of Italian red clover

et al. 2010

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“…For naturalized red clover populations, Hagen and Hamrick (1998) found P = 0.68, A = 1.45 and H e = 0.250. For wild red clover populations Semerikov et al (2002) found H e values that ranged from 0.128 to 0.196 and P ranging from 0.41 to 0.52 and Mosjidis et al (2003) reported P= 74, A = 1.7 and H e = 0.323. For cultivated red clover Kongkiatngam et al (1995) reported H e values ranging from 0.10 to 0.12 in a comparison of two different cultivars and for 34 cultivars of red clover Yu et al (2001) observed mean values of P = 74, A = 1.59 and H e = 0.285.…”

Section: Isozyme Allelic Frequency Datamentioning

confidence: 97%

“…However, RAPD markers are largely limited in the analysis of population structure since a complete genotypic determination is hampered by their dominant nature (Lynch and Milligan 1994). In contrast, isozymes as codominant markers are useful to estimate the allelic frequency and population genetic parameters and have proved reliable in genetic studies with red clover natural populations (Hagen and Hamrick 1998, Lange and Schifino-Wittmann 2000, Semerikov et al 2002, Mosjidis et al 2003 and cultivars (Kongkiatngam et al 1995, Yu et al 2001. Different aspects of the diversity can be analyzed by the use of molecular and biochemical markers.…”

Section: Introductionmentioning

confidence: 99%

Analysis of genetic diversity in the core collection of red clover (Trifolium pratense) with isozyme and RAPD markers

Dias¹,

Pretz²,

Dall'Agnol³

et al. 2008

CBAB

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-The genetic diversity in the red clover USA core collection was measured by RAPD and isozyme markers, comparing different approaches of isozyme data analysis and comparing the results of RAPD with those of isozymes. A total of 15 isozyme loci and 114 RAPD fragments were analyzed. Genetic diversity measured with RAPD and isozymes was high. The analyses of isozymes as binary data was highly correlated with the allele-frequency approach. There was no correlation between the distance matrices of different markers. Isozyme data grouped the accessions of red clover in four groups. The variation evidenced by AMOVA was higher among and within groups in the populations than at the intra-population level.Results showed the high diversity in the red clover core collection and indicated some populations that could be used in breeding programs of the crop in Brazil.

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