Isozyme and RAPD studies in Prosopis glandulosa and P. Velutina (Leguminosae, Mimosoideae)

Abstract

Allozyme and random amplified polymorphic DNA (RAPD) techniques have been compared for their usefulness for genetic and taxonomic studies in Prosopis glandulosa and P. velutina populations. Isozymes and RAPDs yielded similarly high estimates of genetic variability. Genetic structure and differentiation were analyzed through non-hierarchical Wright's F DT . For all populations considered, both markers produced low gene flow (Nm < 1) estimates. When only P. glandulosa populations were analyzed, isozyme data yielded higher gene flow estimates (Nm > 1), in agreement with that expected for conspecific populations. However, in RAPD data the expected reduction in F DT and the increase in Nm were not observed. Correlation between F DT and geographical distance matrices (Mantel test) for all populations was significant (P = 0.02) when based on isozymes, but not so (P = 0.33) when based on RAPDs. No significant associations among genetic and geographical or climatic variables were observed. Two isoenzyme systems (GOT and PRX) enabled us to distinguish between P. glandulosa and P. velutina, but no diagnostic band for recognition of populations or species studied here were detected by RAPD. However, RAPD markers showed higher values for genetic differentiation among conspecific populations of P. glandulosa and a lower coefficient of variation than those obtained from isozymes.

Diversidade genética entre indivíduos de Spondias lutea L. procedentes do baixo são francisco sergipano, por meio de marcadores rapd

“…Estopa et al (2006), estudando a divergência genética entre indivíduos de Eremanthus erythropappus e utilizando 10 oligonucleotídios, obtiveram 56 fragmentos polimórficos, o que foi suficiente para estimar a variabilidade genética entre os indivíduos. Bessega et al (2000), utilizando quatro oligonucleotídios, obtiveram 46 bandas, que foram suficientes para determinar a diversidade genética entre indivíduos de Prosopis glandulosa e P. velutina.…”

Heavy metal and abiotic stress inducible metallothionein isoforms from Prosopis juliflora (SW) D.C. show differences in binding to heavy metals in vitro

“…To the aqueous phase, 0.5 volume of 5 M NaCl and 0.4 volume of isopropyl alcohol was added and centrifuged at 12,000g for 10 min. The genomic DNA pellet was rinsed in wash buVer (10 mM ammonium acetate; 76% ethanol), centrifuged at 12,000 rpm for 10 min, air dried and re-suspended in 10 mM ammonium acetate, 0.25 mM EDTA (pH 8.0) (Bessega et al 2000).…”

Genetic Variation ofZoysiaAs Revealed By Random Amplified Polymorphic Dna (Rapd) and Isozyme Pattern

“…However, the genetic aspect of Zoysia spp., growing in Taiwan, is yet unclear. Isozymes and DNA-based markers are frequently used as molecular markers for taxonomic and systematic analyses of plants Bessega et al, 2000;Garkava et al, 2000;Matos et al, 2001;Ochiai et al, 2001). Many researchers Matos et al, 2001;Ochiai et al, 2001) have pointed out that DNA-based markers, such as random amplified polymorphic DNA (RAPD), were superior to isozymes in detecting genetic diversity.…”